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Literature summary extracted from

  • Duggleby, C.; Williams, P.
    Purification and some properties of the 2-hydroxy-6-oxohepta-2,4-dienoate hydrolase (2-hydroxymuconic semialdehyde hydrolase) encoded by the TOL plasmid pWW0 from Pseudomonas putida mt-2 (1986), J. Gen. Microbiol., 132, 717-726 .
No PubMed abstract available

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.7.1.25 additional information neither EDTA, 2,2'-bipyridyl nor diethyldithiocarbamate have any effect upon enzyme activity. The activity is unaffected by reduced glutathione, 2-mercaptoethanol or L-cysteine (all at 1 mM) Pseudomonas putida

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.7.1.25 Ag+ Ag+ concentrations in the range 0.001-0.01 mM lower the activity to less than 50% of its uninhibited value Pseudomonas putida
3.7.1.25 Cu2+ Cu2+ concentrations in the range 0.001-0.01 mM lower the activity to less than 50% of its uninhibited value Pseudomonas putida
3.7.1.25 iodoacetamide 2 mM iodoacetate inhibits the enzyme activity to 50% or less of its uninhibited value after 15 min incubation with the enzyme at 0°C Pseudomonas putida
3.7.1.25 p-chloromercuribenzoate 0.2 mM p-chloromercuribenzoate inhibits the enzyme activity to 50% or less of its uninhibited value immediately after incubation with the enzyme at 0°C Pseudomonas putida
3.7.1.25 sodium arsenite 0.2 mM sodium arsenite inhibits the enzyme activity to 50% or less of its uninhibited value after 15 min incubation with the enzyme at 0°C Pseudomonas putida

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.7.1.25 0.036
-
(2Z,4E)-2-hydroxy-6-oxohepta-2,4-dienoate at pH 7.5 and 25°C Pseudomonas putida

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.7.1.25 65000
-
gel filtration Pseudomonas putida

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.7.1.25 (2Z,4E)-2-hydroxy-6-oxohepta-2,4-dienoate + H2O Pseudomonas putida
-
(2Z)-2-hydroxypenta-2,4-dienoate + acetate
-
?
3.7.1.25 (2Z,4E)-2-hydroxy-6-oxohepta-2,4-dienoate + H2O Pseudomonas putida ATCC 33015
-
(2Z)-2-hydroxypenta-2,4-dienoate + acetate
-
?

Organism

EC Number Organism UniProt Comment Textmining
3.7.1.25 Pseudomonas putida
-
mt-2
-
3.7.1.25 Pseudomonas putida ATCC 33015
-
mt-2
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.7.1.25 ammonium sulfate precipitation, CM-cellulose column chromatography, DEAE-cellulose column chromatography, Sephadex G-100 gel filtration, and QAE-Sephadex A-50 gel filtration Pseudomonas putida

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.7.1.25 0.58
-
cell-free extract, at pH 7.5 and 25°C Pseudomonas putida
3.7.1.25 48
-
after 82.8fold purification, at pH 7.5 and 25°C Pseudomonas putida

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.7.1.25 (2Z,4E)-2-hydroxy-6-oxohepta-2,4-dienoate + H2O
-
Pseudomonas putida (2Z)-2-hydroxypenta-2,4-dienoate + acetate
-
?
3.7.1.25 (2Z,4E)-2-hydroxy-6-oxohepta-2,4-dienoate + H2O 100% activity Pseudomonas putida (2Z)-2-hydroxypenta-2,4-dienoate + acetate
-
?
3.7.1.25 (2Z,4E)-2-hydroxy-6-oxohepta-2,4-dienoate + H2O
-
Pseudomonas putida ATCC 33015 (2Z)-2-hydroxypenta-2,4-dienoate + acetate
-
?
3.7.1.25 (2Z,4E)-2-hydroxy-6-oxohepta-2,4-dienoate + H2O 100% activity Pseudomonas putida ATCC 33015 (2Z)-2-hydroxypenta-2,4-dienoate + acetate
-
?

Subunits

EC Number Subunits Comment Organism
3.7.1.25 homodimer 31000-33000, SDS-PAGE Pseudomonas putida

Synonyms

EC Number Synonyms Comment Organism
3.7.1.25 2-hydroxymuconic semialdehyde hydrolase
-
Pseudomonas putida
3.7.1.25 HOD hydrolase
-
Pseudomonas putida

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.7.1.25 7.5
-
-
Pseudomonas putida

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.7.1.25 5.5 9 the enzyme is fairly stable to pH values between 5.5 to 9.0, with maximum stability at 7.0 to 8.0. Loss of activity is rapid at pH values below 5.0 and above 9.5 Pseudomonas putida

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.7.1.25 Pseudomonas putida isoelectric focusing
-
4.7