Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Cai, L.; Zhang, M.; Shao, T.; He, Y.; Li, J.; Ren, B.; Zhou, C.
    Effect of introducing disulfide bridges in C-terminal structure on the thermostability of xylanase XynZF-2 from Aspergillus niger (2019), J. Gen. Appl. Microbiol., 65, 240-245.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.2.1.8 EDTA activates Aspergillus niger

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.8 the mutant gene xynZFTA is cloned into pPIC9K and expressed in Pichia pastoris Aspergillus niger

Protein Variants

EC Number Protein Variants Comment Organism
3.2.1.8 T205C/A52C the optimum temperature of the mutant enzyme is improved from 45°C to 60°C, and it retains greater than 90.0% activity (wild-type enzyme retains only 50.0% activity) after treatment at 50°C for 85 min. The optimum pH of mutant xylanase is similar to wild-type enzyme (pH 5.0). The pH stability span (5.0-7.0) of the wild-type enzyme is increased to 3.0-9.0 for the mutant enzyme Aspergillus niger

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.8 Cu2+
-
 Aspergillus niger
3.2.1.8 Fe3+
-
 Aspergillus niger
3.2.1.8 Mn2+
-
 Aspergillus niger
3.2.1.8 Pb2+
-
 Aspergillus niger

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.8 Ca2+ activates Aspergillus niger
3.2.1.8 Zn2+ activates Aspergillus niger

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.8 Aspergillus niger I3QKR9
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.8
-
 Aspergillus niger

Synonyms

EC Number Synonyms Comment Organism
3.2.1.8 xylanase XynZF-2
-
 Aspergillus niger

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.8 45
-
 wild-type enzyme Aspergillus niger
3.2.1.8 60
-
 mutant enzyme T205C/A52C Aspergillus niger

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.8 45
-
 1 h, the wild-type enzyme almost completely loses its activity Aspergillus niger
3.2.1.8 55
-
 1 h, mutant enzyme T205C/A52C retains more than 80% of its initial activity Aspergillus niger

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.8 5
-
 wild-type enzyme and mutant enzyme T205C/A52C Aspergillus niger

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.8 3 9 1 h, 40°C, mutant enzyme T205C/A52C is stable Aspergillus niger
3.2.1.8 5 7 1 h, 40°C, wild-type enzyme is stable Aspergillus niger