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Literature summary extracted from

  • de Almeida, M.; Guimaraes, V.; Falkoski, D.; de Camargo, B.; Fontes-Santana, G.; Maitan-Alfenas, G.; de Rezende, S.
    Purification and characterization of an invertase and a transfructosylase from Aspergillus terreus (2018), J. Food Biochem., 42, e12551.
No PubMed abstract available

Application

EC Number Application Comment Organism
3.2.1.8 food industry application of the extremely thermo- and alkali-stable enzyme for preparation of prebiotic xylooligosaccharides Bacillus subtilis
3.2.1.26 food industry invertase 2 has potential to be applied in food industry since its product, inverted sugar, is used in candies and syrup production, while fructooligosacharides are prebiotics, low calorie and noncariogenic sweeteners Aspergillus terreus

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.8
-
 Bacillus subtilis

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.26 2-mercaptoethanol 2 mM, 50.3% loss of activity Aspergillus terreus
3.2.1.26 Ca2+ 2 mM, 36.7% loss of activity Aspergillus terreus
3.2.1.26 fructose competitive inhibitor for invertase 2 Aspergillus terreus
3.2.1.26 Mg2+ 2 mM, 31.1% loss of activity Aspergillus terreus
3.2.1.26 SDS 2 mM, 89.5% loss of activity Aspergillus terreus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.26 6.2
-
 sucrose 55°C, pH 4.6, invertase 2 Aspergillus terreus
3.2.1.26 9.8
-
 sucrose 55°C, pH 4.6, invertase 1 Aspergillus terreus
3.2.1.26 16.4
-
 raffinose 55°C, pH 4.6, invertase 2 Aspergillus terreus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.8 extracellular
-
 Bacillus subtilis
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.8 35000
-
 SDS-PAGE Bacillus subtilis

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.8 Bacillus subtilis
-
-
-
3.2.1.8 Bacillus subtilis DFR40
-
-
-
3.2.1.26 Aspergillus terreus
-
-
-
3.2.1.26 Aspergillus terreus CCT 4083
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.8
-
 Bacillus subtilis
3.2.1.26
-
 Aspergillus terreus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.8 xylan + H2O
-
 Bacillus subtilis xylose + xylotriose + xylotetraose
-
 ?
3.2.1.8 xylan + H2O
-
 Bacillus subtilis DFR40 xylose + xylotriose + xylotetraose
-
 ?
3.2.1.26 additional information intracellular invertase 2 and invertase 1 do not hydrolyze inulin Aspergillus terreus ?
-
 ?
3.2.1.26 additional information intracellular invertase 2 and invertase 1 do not hydrolyze inulin Aspergillus terreus CCT 4083 ?
-
 ?
3.2.1.26 raffinose + H2O invertase 2 has higher affinity for sucrose than for raffinose Aspergillus terreus beta-D-fructose + melibiose
-
 ?
3.2.1.26 raffinose + H2O invertase 2 has higher affinity for sucrose than for raffinose Aspergillus terreus CCT 4083 beta-D-fructose + melibiose
-
 ?
3.2.1.26 sucrose + H2O invertase 2 also shows transfructosylating activity producing fructooligosaccharides. Invertase 1 does not show transfructosylating activity Aspergillus terreus beta-D-fructose + alpha-D-glucose
-
 ?
3.2.1.26 sucrose + H2O invertase 2 also shows transfructosylating activity producing fructooligosaccharides. Invertase 1 does not show transfructosylating activity Aspergillus terreus CCT 4083 beta-D-fructose + alpha-D-glucose
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.8 ? x * 35000, SDS-PAGE Bacillus subtilis
3.2.1.26 dimer 2 * 32000, invertase 2, SDS-PAGE Aspergillus terreus

Synonyms

EC Number Synonyms Comment Organism
3.2.1.26 invertase 1
-
 Aspergillus terreus
3.2.1.26 invertase 2
-
 Aspergillus terreus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.8 100
-
-
 Bacillus subtilis
3.2.1.26 55
-
 invertase 2 Aspergillus terreus
3.2.1.26 60
-
 invertase 1 Aspergillus terreus

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.2.1.8 50 100 50°C: about 50% of maximal activity, 100°C: maximal activity Bacillus subtilis

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.8 55 85 30 min, enzyme retains more than 70% of its initial activity Bacillus subtilis
3.2.1.26 40
-
 half-life: 44 h, invertase 2 Aspergillus terreus
3.2.1.26 55
-
 half-life: 14.9 h, invertase 2 Aspergillus terreus
3.2.1.26 60
-
 half-life: 2 h, invertase 2 Aspergillus terreus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.8 9
-
-
 Bacillus subtilis
3.2.1.26 3
-
 invertase 1 Aspergillus terreus
3.2.1.26 4.6
-
 invertase 2 Aspergillus terreus

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.8 4 12 18h, 4°C, stable Bacillus subtilis

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.2.1.26 61.5
-
 fructose 55°C, pH 4.6, invertase 2 Aspergillus terreus