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Literature summary extracted from

  • Gorelik, A.; Liu, F.; Illes, K.; Nagar, B.
    Crystal structure of the human alkaline sphingomyelinase provides insights into substrate recognition (2017), J. Biol. Chem., 292, 7087-7094 .
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.1.4.12 additional information bile salts like taurocholate do not directly stimulate catalysis by the enzyme Homo sapiens

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.4.12 expressed in Sf9 insect cells Homo sapiens

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.1.4.12 in complex with phosphocholine, hanging drop vapor diffusion method, using 20% (w/v) PEG 3350 as precipitant Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
3.1.4.12 Y109L the mutation mutant decreases the hydrolysis rate of sphingomyelin and 4-nitrophenylphosphorylcholine 5-10fold compared to the wild type enzyme Homo sapiens
3.1.4.12 Y166L the mutation mutant decreases the hydrolysis rate of sphingomyelin and 4-nitrophenylphosphorylcholine 5-10fold compared to the wild type enzyme Homo sapiens

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.4.12 Zn2+ contains 2 zinc ions Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.4.12 sphingomyelin + H2O Homo sapiens
-
 ceramide + phosphocholine
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.4.12 Homo sapiens Q6UWV6
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.4.12 Ni-NTA resin column chromatography and Superdex 200 gel filtration Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.4.12 4-nitrophenyl phosphorylcholine + H2O
-
 Homo sapiens 4-nitrophenol + phosphorylcholine
-
 ?
3.1.4.12 bis(4-nitrophenyl)phosphate + H2O
-
 Homo sapiens ?
-
 ?
3.1.4.12 additional information no activity with 4-nitrophenyl-TMP Homo sapiens ?
-
 ?
3.1.4.12 sphingomyelin + H2O
-
 Homo sapiens ceramide + phosphocholine
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.1.4.12 alk-SMase
-
 Homo sapiens
3.1.4.12 alkaline sphingomyelinase
-
 Homo sapiens
3.1.4.12 ENPP7
-
 Homo sapiens
3.1.4.12 NPP7
-
 Homo sapiens