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Literature summary extracted from
- Identification of the gene encoding isoprimeverose-producing oligoxyloglucan hydrolase in Aspergillus oryzae (2016), J. Biol. Chem., 291, 5080-5087 .
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 3.2.1.120 | expressed in Pichia pastoris | Aspergillus oryzae |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.2.1.120 | 1.79 | - |
alpha-D-Xyl-(1->6)-beta-D-Glc-(1->4)-[alpha-D-Xyl-(1->6)]-beta-D-Glc-(1->4)-beta-D-Glc | pH 4.5, 60°C | Aspergillus oryzae |  |
| 3.2.1.120 | 2.35 | - |
alpha-D-Xyl-(1->6)-beta-D-Glc-(1->4)-[beta-D-Gal-(1->2)-alpha-D-Xyl-(1->6)]-beta-D-Glc-(1->4)-[beta-D-Gal-(1->2)-alpha-D-Xyl-(1->6)]-beta-D-Glc | pH 4.5, 60°C | Aspergillus oryzae | |
| 3.2.1.120 | 4.03 | - |
alpha-D-Xyl-(1->6)-beta-D-Glc | pH 4.5, 60°C | Aspergillus oryzae | |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.2.1.120 | oligoxyloglucan + H2O | Aspergillus oryzae | the enzyme is involved in utilization of oligoxyloglucans as carbon sources | isoprimeverose + ? | - |
? | |
| 3.2.1.120 | oligoxyloglucan + H2O | Aspergillus oryzae ATCC 42149 | the enzyme is involved in utilization of oligoxyloglucans as carbon sources | isoprimeverose + ? | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.2.1.120 | Aspergillus oryzae | Q2U8V9 | - |
- |
| 3.2.1.120 | Aspergillus oryzae ATCC 42149 | Q2U8V9 | - |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.2.1.120 | - |
Aspergillus oryzae |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.120 | 0.23 | - |
substrate: 4-nitrophenyl beta-D-glucopyranoside, pH 4.5, 60°C | Aspergillus oryzae |
| 3.2.1.120 | 1.32 | - |
substrate: 4-nitrophenyl beta-D-galactopyranoside, pH 4.5, 60°C | Aspergillus oryzae |
| 3.2.1.120 | 226 | - |
substrate: alpha-D-Xyl-(1->6)-beta-D-Glc, pH 4.5, 60°C | Aspergillus oryzae |
| 3.2.1.120 | 296 | - |
substrate: alpha-D-Xyl-(1->6)-beta-D-Glc-(1->4)-[beta-D-Gal-(1->2)-alpha-D-Xyl-(1->6)]-beta-D-Glc-(1->4)-[alpha-D-Xyl-(1->6)]-beta-D-Glc, pH 4.5, 60°C | Aspergillus oryzae |
| 3.2.1.120 | 297 | - |
substrate: alpha-D-Xyl-(1->6)-beta-D-Glc-(1->4)-[beta-D-Gal-(1->2)-alpha-D-Xyl-(1->6)]-beta-D-Glc-(1->4)-[beta-D-Gal-(1->2)-alpha-D-Xyl-(1->6)]-beta-D-Glc, pH 4.5, 60°C | Aspergillus oryzae |
| 3.2.1.120 | 422 | - |
substrate: alpha-D-Xyl-(1->6)-beta-D-Glc-(1->4)-[alpha-D-Xyl-(1->6)]-beta-D-Glc-(1->4)-beta-D-Glc, pH 4.5, 60°C | Aspergillus oryzae |
| 3.2.1.120 | 427 | - |
substrate: alpha-D-Xyl-(1->6)-beta-D-Glc-(1->4)-[alpha-D-Xyl-(1->6)]-beta-D-Glc-(1->4)-[alpha-D-Xyl-(1->6)]-beta-D-Glc-(1->4)-beta-D-Glcol, pH 4.5, 60°C | Aspergillus oryzae |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.2.1.120 | 4-nitrophenyl beta-D-galactopyranoside + H2O | - |
Aspergillus oryzae | 4-nitrophenol + beta-D-galactopyranose | - |
? | |
| 3.2.1.120 | 4-nitrophenyl beta-D-galactopyranoside + H2O | - |
Aspergillus oryzae ATCC 42149 | 4-nitrophenol + beta-D-galactopyranose | - |
? | |
| 3.2.1.120 | 4-nitrophenyl beta-D-glucopyranoside + H2O | - |
Aspergillus oryzae | 4-nitrophenol + beta-D-glucopyranose | - |
? | |
| 3.2.1.120 | 4-nitrophenyl beta-D-glucopyranoside + H2O | - |
Aspergillus oryzae ATCC 42149 | 4-nitrophenol + beta-D-glucopyranose | - |
? | |
| 3.2.1.120 | alpha-D-Xyl-(1->6)-beta-D-Glc + H2O | - |
Aspergillus oryzae | ? | - |
? | |
| 3.2.1.120 | alpha-D-Xyl-(1->6)-beta-D-Glc + H2O | - |
Aspergillus oryzae ATCC 42149 | ? | - |
? | |
| 3.2.1.120 | alpha-D-Xyl-(1->6)-beta-D-Glc-(1->4)-[alpha-D-Xyl-(1->6)]-beta-D-Glc-(1->4)-beta-D-Glc + H2O | - |
Aspergillus oryzae | ? | - |
? | |
| 3.2.1.120 | alpha-D-Xyl-(1->6)-beta-D-Glc-(1->4)-[alpha-D-Xyl-(1->6)]-beta-D-Glc-(1->4)-[alpha-D-Xyl-(1->6)]-beta-D-Glc-(1->4)-beta-D-Glc + H2O | - |
Aspergillus oryzae | ? | - |
? | |
| 3.2.1.120 | alpha-D-Xyl-(1->6)-beta-D-Glc-(1->4)-[alpha-D-Xyl-(1->6)]-beta-D-Glc-(1->4)-[alpha-D-Xyl-(1->6)]-beta-D-Glc-(1->4)-beta-D-Glc + H2O | - |
Aspergillus oryzae | alpha-D-Xyl-(1->6)-beta-D-Glc-(1->4)-[alpha-D-Xyl-(1->6)]-beta-D-Glc-(1->4)-beta-D-Glc + alpha-D-Xyl-(1->6)-beta-D-Glc | - |
? | |
| 3.2.1.120 | alpha-D-Xyl-(1->6)-beta-D-Glc-(1->4)-[beta-D-Gal-(1->2)-alpha-D-Xyl-(1->6)]-beta-D-Glc-(1->4)-[beta-D-Gal-(1->2)-alpha-D-Xyl-(1->6)]-beta-D-Glc + H2O | - |
Aspergillus oryzae | ? | - |
? | |
| 3.2.1.120 | oligoxyloglucan + H2O | the enzyme is involved in utilization of oligoxyloglucans as carbon sources | Aspergillus oryzae | isoprimeverose + ? | - |
? | |
| 3.2.1.120 | oligoxyloglucan + H2O | the enzyme recognizes and releases isoprimeverose (alpha-D-xylopyranose-(1,6)-D-glucopyranose) units from the non-reducing ends of oligoxyloglucans. Xylopyranosyl-branching at the non-reducing ends was vital for IPase activity, and galactosylation at alpha-(1,6)-linked xylopyranosyl side chain completely abolishes activity. Hepta-oligoxyloglucan saccharide (Xyl3Glc4) substrate is preferred over tri-(Xyl1Glc2) and tetra-(Xyl2Glc2) oligoxyloglucan saccharides substrates. IpeA transfers isoprimeverose units to other saccharides, indicating transglycosylation activity | Aspergillus oryzae | isoprimeverose + ? | - |
? | |
| 3.2.1.120 | oligoxyloglucan + H2O | the enzyme is involved in utilization of oligoxyloglucans as carbon sources | Aspergillus oryzae ATCC 42149 | isoprimeverose + ? | - |
? | |
| 3.2.1.120 | oligoxyloglucan + H2O | the enzyme recognizes and releases isoprimeverose (alpha-D-xylopyranose-(1,6)-D-glucopyranose) units from the non-reducing ends of oligoxyloglucans. Xylopyranosyl-branching at the non-reducing ends was vital for IPase activity, and galactosylation at alpha-(1,6)-linked xylopyranosyl side chain completely abolishes activity. Hepta-oligoxyloglucan saccharide (Xyl3Glc4) substrate is preferred over tri-(Xyl1Glc2) and tetra-(Xyl2Glc2) oligoxyloglucan saccharides substrates. IpeA transfers isoprimeverose units to other saccharides, indicating transglycosylation activity | Aspergillus oryzae ATCC 42149 | isoprimeverose + ? | - |
? | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.2.1.120 | IPase | - |
Aspergillus oryzae |
| 3.2.1.120 | IpeA | - |
Aspergillus oryzae |
| 3.2.1.120 | isoprimeverose-producing oligoxyloglucan hydrolase | - |
Aspergillus oryzae |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.2.1.120 | 532 | - |
alpha-D-Xyl-(1->6)-beta-D-Glc | pH 4.5, 60°C | Aspergillus oryzae | |
| 3.2.1.120 | 661 | - |
alpha-D-Xyl-(1->6)-beta-D-Glc-(1->4)-[beta-D-Gal-(1->2)-alpha-D-Xyl-(1->6)]-beta-D-Glc-(1->4)-[beta-D-Gal-(1->2)-alpha-D-Xyl-(1->6)]-beta-D-Glc | pH 4.5, 60°C | Aspergillus oryzae | |
| 3.2.1.120 | 1216 | - |
alpha-D-Xyl-(1->6)-beta-D-Glc-(1->4)-[alpha-D-Xyl-(1->6)]-beta-D-Glc-(1->4)-beta-D-Glc | pH 4.5, 60°C | Aspergillus oryzae | |
Expression
| EC Number | Organism | Comment | Expression |
|---|---|---|---|
| 3.2.1.120 | Aspergillus oryzae | the enzyme is expressed in xylose and xyloglucan media and is strongly induced in the presence of xyloglucan endo-xyloglucanase-hydrolyzed products | up |
General Information
| EC Number | General Information | Comment | Organism |
|---|---|---|---|
| 3.2.1.120 | physiological function | the enzyme is involved in utilization of oligoxyloglucans as carbon sources | Aspergillus oryzae |
kcat/KM [mM/s]
| EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.2.1.120 | 132 | - |
alpha-D-Xyl-(1->6)-beta-D-Glc | pH 4.5, 60°C | Aspergillus oryzae | |
| 3.2.1.120 | 282 | - |
alpha-D-Xyl-(1->6)-beta-D-Glc-(1->4)-[beta-D-Gal-(1->2)-alpha-D-Xyl-(1->6)]-beta-D-Glc-(1->4)-[beta-D-Gal-(1->2)-alpha-D-Xyl-(1->6)]-beta-D-Glc | pH 4.5, 60°C | Aspergillus oryzae | |
| 3.2.1.120 | 678 | - |
alpha-D-Xyl-(1->6)-beta-D-Glc-(1->4)-[alpha-D-Xyl-(1->6)]-beta-D-Glc-(1->4)-[alpha-D-Xyl-(1->6)]-beta-D-Glc-(1->4)-beta-D-Glc | pH 4.5, 60°C | Aspergillus oryzae | |
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