Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Khandokar, Y.; Srivastava, P.; Sarker, S.; Swarbrick, C.; Aragao, D.; Cowieson, N.; Forwood, J.
    Structural and functional characterization of the PaaI thioesterase from Streptococcus pneumoniae reveals a dual specificity for phenylacetyl-CoA and medium-chain fatty Acyl-CoAs and a novel CoA-induced fit mechanism (2016), J. Biol. Chem., 291, 1866-1876 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.2.25 expressed in Escherichia coli BL21(DE3) cells Streptococcus pneumoniae

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.1.2.25 hanging drop vapor diffusion method. Crystals of wild type enzyme and mutants N37A, D52A, and T68A are obtained in conditions composed of 200 mM ammonium tartrate dibasic, 20% (w/v) PEG 3350, 100 mM Tris, pH 7.5; 200 mM lithium sulfate, 100 mM Tris-HCl, 30% (w/v) PEG 4000, 200 mM magnesium chloride, PEG 400, 100 mM HEPES, pH 7.5; 200 mM magnesium chloride, 14% (w/v) PEG 3350, 100 mM Tris, pH 6.0, respectively Streptococcus pneumoniae

Protein Variants

EC Number Protein Variants Comment Organism
3.1.2.25 D52A the mutant has completely abolished activity Streptococcus pneumoniae
3.1.2.25 N37A the mutant has completely abolished activity Streptococcus pneumoniae
3.1.2.25 T68A the mutant shows a marked reduction in activity compared to the wild type enzyme Streptococcus pneumoniae

Organism

EC Number Organism UniProt Comment Textmining
3.1.2.25 Streptococcus pneumoniae A0A0M3KL39
-
-
3.1.2.25 Streptococcus pneumoniae ATCC BAA-334 A0A0M3KL39
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.2.25 HisTrap affinity column chromatography and gel filtration Streptococcus pneumoniae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.2.25 acetyl-CoA + H2O very low activity Streptococcus pneumoniae acetate + CoA
-
 ?
3.1.2.25 acetyl-CoA + H2O very low activity Streptococcus pneumoniae ATCC BAA-334 acetate + CoA
-
 ?
3.1.2.25 arachidonyl-CoA + H2O very low activity Streptococcus pneumoniae arachidonate + CoA
-
 ?
3.1.2.25 butyryl-CoA + H2O low activity Streptococcus pneumoniae butanoate + CoA
-
 ?
3.1.2.25 butyryl-CoA + H2O low activity Streptococcus pneumoniae ATCC BAA-334 butanoate + CoA
-
 ?
3.1.2.25 decanoyl-CoA + H2O highest activity Streptococcus pneumoniae decanoate + CoA
-
 ?
3.1.2.25 dodecanoyl-CoA + H2O high activity Streptococcus pneumoniae dodecanoate + CoA
-
 ?
3.1.2.25 hexanoyl-CoA + H2O
-
 Streptococcus pneumoniae hexanoate + CoA
-
 ?
3.1.2.25 additional information no activity with malonyl-CoA, palmitoyl-CoA, and stearoyl-CoA Streptococcus pneumoniae ?
-
 ?
3.1.2.25 additional information no activity with malonyl-CoA, palmitoyl-CoA, and stearoyl-CoA Streptococcus pneumoniae ATCC BAA-334 ?
-
 ?
3.1.2.25 myristoyl-CoA + H2O very low activity Streptococcus pneumoniae myristate + CoA
-
 ?
3.1.2.25 myristoyl-CoA + H2O very low activity Streptococcus pneumoniae ATCC BAA-334 myristate + CoA
-
 ?
3.1.2.25 octanoyl-CoA + H2O high activity Streptococcus pneumoniae octanoate + CoA
-
 ?
3.1.2.25 palmitoyl-CoA + H2O
-
 Streptococcus pneumoniae palmitate + CoA
-
 ?
3.1.2.25 palmitoyl-CoA + H2O
-
 Streptococcus pneumoniae ATCC BAA-334 palmitate + CoA
-
 ?
3.1.2.25 phenylacetyl-CoA + H2O low activity. The wild type enzyme displays a greater activity for phenylacetyl-CoA than very short and long chain saturated fatty acyl-CoA substrates Streptococcus pneumoniae phenylacetate + CoA
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.2.25 tetramer
-
 Streptococcus pneumoniae

Synonyms

EC Number Synonyms Comment Organism
3.1.2.25 PaaI
-
 Streptococcus pneumoniae
3.1.2.25 PaaI thioesterase
-
 Streptococcus pneumoniae

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.1.2.25 6.5
-
 phenylacetyl-CoA wild type enzyme, at pH 7.6 and 21°C Streptococcus pneumoniae
3.1.2.25 32.8
-
 decanoyl-CoA wild type enzyme, at pH 7.6 and 21°C Streptococcus pneumoniae

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.1.2.25 72
-
 phenylacetyl-CoA wild type enzyme, at pH 7.6 and 21°C Streptococcus pneumoniae
3.1.2.25 180
-
 decanoyl-CoA wild type enzyme, at pH 7.6 and 21°C Streptococcus pneumoniae