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Literature summary extracted from

  • Pascal, S.; Taton, M.; Rahier, A.
    Plant sterol biosynthesis. Identification and characterization of two distinct microsomal oxidative enzymatic systems involved in sterol C4-demethylation (1993), J. Biol. Chem., 268, 11639-11654 .
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.14.18.10 cyanide
-
 Zea mays
1.14.18.10 cytochrome c
-
 Zea mays
1.14.18.10 menadione 39% residual activity at 0.05 mM Zea mays
1.14.18.10 additional information not inhibited by N3-, CO (saturated), LAB 170250F, and tetcyclacis Zea mays
1.14.18.10 orthophenanthroline 44% residual activity at 1 mM Zea mays
1.14.18.11 cyanide
-
 Zea mays
1.14.18.11 cytochrome c
-
 Zea mays
1.14.18.11 menadione 34% residual activity at 0.05 mM Zea mays
1.14.18.11 additional information not inhibited by N3-, CO (saturated), LAB 170250F, and tetcyclacis Zea mays
1.14.18.11 orthophenanthroline 47% residual activity at 1 mM Zea mays
1.14.18.11 stigmastan-3beta,5alpha,6alpha-triol effectively inhibited by stigmastan-3beta,5alpha,6alpha-triol Zea mays

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.14.18.10 microsome
-
 Zea mays
-
-
1.14.18.11 microsome
-
 Zea mays
-
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.18.10 24-methylidenecycloartanol + 6 ferrocytochrome b5 + 3 O2 + 6 H+ Zea mays overall reaction 3beta-hydroxy-4beta,14alpha-dimethyl-9beta,19-cyclo-5alpha-ergost-24(241)-ene-4alpha-carboxylate + 6 ferricytochrome b5 + 4 H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.14.18.10 Zea mays
-
-
-
1.14.18.11 Zea mays
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.18.10 24(25)-dihydrocycloartenol + NAD(P)H + H+ 30% activity compared to 24-methylidenecycloartanol Zea mays ?
-
 ?
1.14.18.10 24-methylidenecycloartanol + 6 ferrocytochrome b5 + 3 O2 + 6 H+ overall reaction Zea mays 3beta-hydroxy-4beta,14alpha-dimethyl-9beta,19-cyclo-5alpha-ergost-24(241)-ene-4alpha-carboxylate + 6 ferricytochrome b5 + 4 H2O
-
 ?
1.14.18.10 24-methylidenecycloartanol + NAD(P)H + O2 + H+ 100% activity Zea mays 4alpha-hydroxymethyl-4beta,14alpha-dimethyl-5alpha-ergosta-9beta,19-cyclo-24(241)-en-3beta-ol + NAD(P)+ + H2O
-
 ?
1.14.18.10 24zeta-methylcycloartanol + NAD(P)H + H+ 43% activity compared to 24-methylidenecycloartanol Zea mays ?
-
 ?
1.14.18.10 cyclolaudenol + NAD(P)H + H+ 56% activity compared to 24-methylidenecycloartanol Zea mays ?
-
 ?
1.14.18.10 additional information no activity with cyclobranol, cycloartenol, 24-methylenelanosterol, lanosterol, 24(25)-dihydrolanosterol, parkeol, 4,4-dimethyl-7,22-ergostadienol, beta-amyrine, 4alpha,14alpha,dimethyl-5alpha-cholesta-7-en-3beta-ol, obtusifoliol, 24zeta(241)-dihydroobtusifoliol, 29-norlanosterol, 24(25)-dihydro-29-norlanosterol, 4alpha-dimethyl-5alpha-cholestanol, 4alpha,14alpha,24zeta-trimethyl-5alpha-cholesta-9(11)-en-3beta-ol, and 4beta-24zeta(241)-dihydrocycloeucalenol Zea mays ?
-
 ?
1.14.18.11 24-ethylidenelophenol + 6 ferrocytochrome b5 + 3 O2 + 6 H+
-
 Zea mays (3beta,4alpha,5alpha,24Z)-3-hydroxystigmasta-7,24(28)-diene-4-carboxylic acid + 6 ferricytochrome b5 + 4 H2O
-
 ir
1.14.18.11 24-ethylidenelophenol + NAD(P)H + O2 + H+ 100% activity Zea mays ?
-
 ?
1.14.18.11 24-methylidenelophenol + NAD(P)H + H+ 59% activity compared to 24-ethylidenelophenol Zea mays ?
-
 ?
1.14.18.11 24zeta(241)-dihydrocycloeucalenol + NAD(P)H + H+ 10% activity compared to 24-ethylidenelophenol Zea mays ?
-
 ?
1.14.18.11 cycloeucalenol + NAD(P)H + H+ 10% activity compared to 24-ethylidenelophenol Zea mays ?
-
 ?
1.14.18.11 lophenol + NAD(P)H + H+ 38% activity compared to 24-ethylidenelophenol Zea mays ?
-
 ?
1.14.18.11 additional information no activity with cyclobranol, cycloartenol, 24-methylenelanosterol, lanosterol, 24(25)-dihydrolanosterol, parkeol, 4,4-dimethyl-7,22-ergostadienol, beta-amyrine, 4alpha,14alpha,dimethyl-5alpha-cholesta-7-en-3beta-ol, obtusifoliol, 24zeta(241)-dihydroobtusifoliol, 29-norlanosterol, 24(25)-dihydro-29-norlanosterol, 4alpha-dimethyl-5alpha-cholestanol, 4alpha,14alpha,24zeta-trimethyl-5alpha-cholesta-9(11)-en-3beta-ol, and 4beta-24zeta(241)-dihydrocycloeucalenol Zea mays ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.14.18.10 4,4-dimethylsterol-oxidase
-
 Zea mays
1.14.18.11 4alpha-methyl oxidase
-
 Zea mays
1.14.18.11 4alpha-methylsterol C4 oxidase
-
 Zea mays

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.18.10 NADH required Zea mays
1.14.18.10 NADPH required Zea mays
1.14.18.11 NADH required Zea mays
1.14.18.11 NADPH required Zea mays

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
1.14.18.10 0.01
-
 using 24-methylenecycloartanol as substrate, at 30°C, pH not specified in the publication Zea mays cyanide
1.14.18.10 0.03
-
 using 24-methylenecycloartanol as substrate, at 30°C, pH not specified in the publication Zea mays cytochrome c
1.14.18.11 0.035
-
 using 24-ethylidenelophenol as substrate, at 30°C, pH not specified in the publication Zea mays cytochrome c
1.14.18.11 0.05
-
 using 24-ethylidenelophenol as substrate, at 30°C, pH not specified in the publication Zea mays cyanide