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Literature summary extracted from

  • Boukhris, I.; Farhat-Khemakhem, A.; Bouchaala, K.; Virolle, M.J.; Chouayekh, H.
    Cloning and characterization of the first actinomycete beta-propeller phytase from Streptomyces sp. US42 (2016), J. Basic Microbiol., 56, 1080-1089 .
    View publication on PubMed

Application

EC Number Application Comment Organism
3.1.3.8 agriculture enzyme PHY US42 can be used as feed additive in combination with an acid phytase for monogastric animals Streptomyces sp.

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.3.8 gene phy, DNA and amino acid sequence determination and analysis, sequence comparisons Streptomyces sp.

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.3.8 additional information enzyme PHYUS42 exhibits high stability after incubation at 37°C for 1 h in the presence of bovine bile and digestive proteases like pepsin, trypsin, and chymotrypsin. PHY US42 maintains 90% of its activity after incubation for 1 h in the presence of 0.5% bovine bile Streptomyces sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.3.8 1.073
-
 myo-inositol hexakisphosphate pH 7.0, 65°C Streptomyces sp.

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.3.8 extracellular
-
 Streptomyces sp.
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.3.8 Ca2+ the activity is calcium-dependent, best at 1 mM. Four Ca2+ ions bind to the active Ca2+ binding sites (C3, C5, C6, C7) and create an ideal conformation and charge distribution for the substrate Streptomyces sp.

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.3.8 myo-inositol hexakisphosphate + H2O Streptomyces sp.
-
 1D-myo-inositol 1,2,4,5,6-pentakisphosphate + phosphate
-
 ?
3.1.3.8 myo-inositol hexakisphosphate + H2O Streptomyces sp. US42
-
 1D-myo-inositol 1,2,4,5,6-pentakisphosphate + phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.3.8 Streptomyces sp. A0A0F7VP60 isolated from Tunisian soils
-
3.1.3.8 Streptomyces sp. US42 A0A0F7VP60 isolated from Tunisian soils
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.3.8 native enzyme from cell culture medium 4.2fold by ammonium sulfate frcationation and dialysis Streptomyces sp.

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.3.8 2.1
-
 partially purified enzyme, pH 7.0, 65°C Streptomyces sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.3.8 myo-inositol hexakisphosphate + H2O
-
 Streptomyces sp. 1D-myo-inositol 1,2,4,5,6-pentakisphosphate + phosphate
-
 ?
3.1.3.8 myo-inositol hexakisphosphate + H2O
-
 Streptomyces sp. US42 1D-myo-inositol 1,2,4,5,6-pentakisphosphate + phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.3.8 ? x * 45795, sequence calcualtion, x * 48000, SDS-PAGE Streptomyces sp.

Synonyms

EC Number Synonyms Comment Organism
3.1.3.8 beta-propeller phytase
-
 Streptomyces sp.
3.1.3.8 Phy
-
 Streptomyces sp.
3.1.3.8 PHY US42
-
 Streptomyces sp.

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.3.8 65
-
-
 Streptomyces sp.

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.1.3.8 35 80 activity range, profile overview Streptomyces sp.

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.3.8 additional information
-
 the temperature stability is increased by Ca2+ Streptomyces sp.
3.1.3.8 75
-
 enzyme PHY US42 maintains 80% activity after 10 min in the presence of 5 mM CaCl2, whereas in the absence of calcium, PHY US42 activity is completely abolished after 2 min of incubation Streptomyces sp.

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.1.3.8 3
-
 myo-inositol hexakisphosphate pH 7.0, 65°C Streptomyces sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.3.8 7 8
-
 Streptomyces sp.

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.1.3.8 6 9 activity range, inactive below and above, profile overview Streptomyces sp.

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.1.3.8 5 10 completely stable at Streptomyces sp.

General Information

EC Number General Information Comment Organism
3.1.3.8 evolution molecular modeling of PHY US42 indicates that this phytase belongs to the group of beta-propeller phytases that are usually calcium-dependent Streptomyces sp.
3.1.3.8 additional information amino acid sequence analysis and homology structure modeling Streptomyces sp.

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.1.3.8 2.8
-
 myo-inositol hexakisphosphate pH 7.0, 65°C Streptomyces sp.