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Literature summary extracted from

  • Jacewicz, A.; Shuman, S.
    Biochemical characterization of Mycobacterium smegmatis RnhC (MSMEG_4305), a bifunctional enzyme composed of autonomous N-terminal type I RNase H and C-terminal acid phosphatase domains (2015), J. Bacteriol., 197, 2489-2498 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.3.2 expressed in Escherichia coli BL21(DE3) RIPL cells Mycolicibacterium smegmatis

Protein Variants

EC Number Protein Variants Comment Organism
3.1.3.2 D73N the mutation effaces RNase H activity but has virtually no effect on the acid phosphatase activity of the RnhC protein Mycolicibacterium smegmatis
3.1.3.2 H173A the mutation does not affect RNase H activity but abolishes the acid phosphatase activity of the RnhC protein Mycolicibacterium smegmatis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.3.2 additional information the acid phosphatase activity does not use a divalent cation cofactor Mycolicibacterium smegmatis

Organism

EC Number Organism UniProt Comment Textmining
3.1.3.2 Mycolicibacterium smegmatis
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.3.2 His60-Ni Superflow resin column chromatography, Ni-NTA agarose column chromatography, Q-Sepharose column chromatography, and Superdex 200 gel filtration Mycolicibacterium smegmatis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.3.2 4-nitrophenyl phosphate + H2O
-
 Mycolicibacterium smegmatis 4-nitrophenol + phosphate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.1.3.2 rnhC bifunctional enzyme with RNase H (N-terminus) and acid phosphatase (C-terminus) activities Mycolicibacterium smegmatis

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.1.3.2 0.08
-
 4-nitrophenyl phosphate at pH 5.5, temperature not specified in the publication Mycolicibacterium smegmatis
3.1.3.2 0.33
-
 4-nitrophenyl phosphate at pH 4.5, temperature not specified in the publication Mycolicibacterium smegmatis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.3.2 4.5
-
-
 Mycolicibacterium smegmatis