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Literature summary extracted from
- Dietary phenolic compounds selectively inhibit the individual subunits of maltase-glucoamylase and sucrase-isomaltase with the potential of modulating glucose release (2015), J. Agric. Food Chem., 63, 3873-3879 .
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 3.2.1.3 | recombinant expression of the glucoamylase subunit Ct-MGAM splice form N20 and the sucrase subunit Ct-SI in Spodoptera frugiperda Sf9 cells using the baculovirus transfection system | Mus musculus |
| 3.2.1.48 | expression in Drosophila S2 cells | Homo sapiens |
| 3.2.1.48 | expression in Drosophila S2 cells | Mus musculus |
| 3.2.1.48 | recombinant expression in a baculovirus-Sf9 insect cell system | Homo sapiens |
| 3.2.1.48 | recombinant expression in a baculovirus-Sf9 insect cell system | Mus musculus |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.2.1.3 | (+)-catechin | noncompetitive inhibition | Mus musculus |  |
| 3.2.1.3 | caffeic acid | noncompetitive inhibition | Mus musculus | |
| 3.2.1.3 | chlorogenic acid | noncompetitive inhibition | Mus musculus | |
| 3.2.1.3 | epigallocatechin gallate | EGCG, noncompetitive inhibition | Mus musculus | |
| 3.2.1.3 | gallic acid | noncompetitive inhibition | Mus musculus | |
| 3.2.1.3 | additional information | kinetics and mechanism of inhibition of the recombinant glucoamylase subunit Ct-MGAM and sucrase subunit Ct-SI, overview | Mus musculus | |
| 3.2.1.48 | (+)-catechin | noncompetitive | Homo sapiens | |
| 3.2.1.48 | (+)-catechin | noncompetitive | Mus musculus | |
| 3.2.1.48 | (-)-epigallocatechin gallate | noncompetitive | Homo sapiens | |
| 3.2.1.48 | (-)-epigallocatechin gallate | noncompetitive | Mus musculus | |
| 3.2.1.48 | caffeic acid | noncompetitive | Homo sapiens | |
| 3.2.1.48 | caffeic acid | noncompetitive | Mus musculus | |
| 3.2.1.48 | chlorogenic acid | noncompetitive | Homo sapiens | |
| 3.2.1.48 | chlorogenic acid | noncompetitive | Mus musculus | |
| 3.2.1.48 | gallic acid | noncompetitive | Homo sapiens | |
| 3.2.1.48 | gallic acid | noncompetitive | Mus musculus | |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 3.2.1.3 | membrane | - |
Mus musculus | 16020 | - |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.2.1.3 | starch + H2O | Mus musculus | - |
? | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.2.1.3 | Mus musculus | Q6XK24 | - |
- |
| 3.2.1.48 | Homo sapiens | P14410 | - |
- |
| 3.2.1.48 | Mus musculus | B5THE3 | - |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.2.1.3 | recombinant glucoamylase subunit Ct-MGAM splice form N20 and subunit Ct-SI from Spodoptera frugiperda Sf9 cells | Mus musculus |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.2.1.3 | starch + H2O | - |
Mus musculus | ? | - |
? | |
| 3.2.1.3 | starch + H2O | soluble starch | Mus musculus | ? | - |
? | |
| 3.2.1.48 | maltose + H2O | - |
Homo sapiens | alpha-D-glucose + D-glucose | - |
? | |
| 3.2.1.48 | maltose + H2O | - |
Mus musculus | alpha-D-glucose + D-glucose | - |
? | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.2.1.3 | glucoamylase | - |
Mus musculus |
| 3.2.1.48 | sucrase-isomaltase | - |
Homo sapiens |
| 3.2.1.48 | sucrase-isomaltase | - |
Mus musculus |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.3 | 37 | - |
assay at | Mus musculus |
IC50 Value
| EC Number | IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
|---|---|---|---|---|---|---|
| 3.2.1.3 | 0.00151 | - |
pH not specified in the publication, 37°C, recombinant glucoamylase subunit Ct-SI | Mus musculus | epigallocatechin gallate | |
| 3.2.1.3 | 0.0017 | - |
pH not specified in the publication, 37°C, recombinant glucoamylase subunit Ct-MGAM | Mus musculus | epigallocatechin gallate | |
| 3.2.1.3 | 0.0018 | - |
pH not specified in the publication, 37°C, recombinant glucoamylase subunit Ct-SI | Mus musculus | chlorogenic acid | |
| 3.2.1.3 | 0.003 | - |
pH not specified in the publication, 37°C, recombinant glucoamylase subunit Ct-MGAM | Mus musculus | chlorogenic acid | |
| 3.2.1.3 | 0.0204 | - |
pH not specified in the publication, 37°C, recombinant glucoamylase subunit Ct-MGAM | Mus musculus | (+)-catechin | |
| 3.2.1.3 | 0.0216 | - |
pH not specified in the publication, 37°C, recombinant glucoamylase subunit Ct-SI | Mus musculus | (+)-catechin | |
| 3.2.1.3 | 0.0249 | - |
pH not specified in the publication, 37°C, recombinant glucoamylase subunit Ct-MGAM | Mus musculus | gallic acid | |
| 3.2.1.3 | 0.0313 | - |
pH not specified in the publication, 37°C, recombinant glucoamylase subunit Ct-SI | Mus musculus | gallic acid | |
| 3.2.1.3 | 0.0565 | - |
pH not specified in the publication, 37°C, recombinant glucoamylase subunit Ct-MGAM | Mus musculus | caffeic acid | |
| 3.2.1.3 | 0.0582 | - |
pH not specified in the publication, 37°C, recombinant glucoamylase subunit Ct-SI | Mus musculus | caffeic acid | |
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