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Literature summary extracted from
- A novel phytase characterized by thermostability and high pH tolerance from rice phyllosphere isolated Bacillus subtilis B.S.46 (2016), J. Adv. Res., 7, 381-390 .
Application
| EC Number | Application | Comment | Organism |
|---|---|---|---|
| 3.1.3.8 | additional information | the enzyme is useful as animal feed additive, in dephytinization of food ingredients, and bioremediation of phosphorous pollution in the environment | Bacillus subtilis |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.1.3.8 | Ca2+ | 47% activation at 1 mM, 54% inhibition at 5 mM, 90% at 10 mM | Bacillus subtilis |  |
| 3.1.3.8 | Cu2+ | 8% inhibition at 1 mM, 92% inhibition at 5 mM; complete inhibition | Bacillus subtilis | |
| 3.1.3.8 | Fe2+ | 30% inhibition at 1 mM, complete inhibition at 2 mM; complete inhibition | Bacillus subtilis | |
| 3.1.3.8 | Hg2+ | complete inhibition; complete inhibition at 1-10 mM | Bacillus subtilis | |
| 3.1.3.8 | K+ | about 10% inhibition at 2-10 mM | Bacillus subtilis | |
| 3.1.3.8 | Mg2+ | 6% inhibition at 1 mM, 90% inhibition at 5 mM; complete inhibition | Bacillus subtilis | |
| 3.1.3.8 | Mn2+ | 92% inhibition at 1 mM, complete inhibition at 5 mM; complete inhibition | Bacillus subtilis | |
| 3.1.3.8 | Zn2+ | 63% inhibition at 1 mM, 88% inhibition at 5 mM; complete inhibition | Bacillus subtilis | |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 3.1.3.8 | extracellular | - |
Bacillus subtilis | - |
- |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.1.3.8 | Ca2+ | 47% activation at 1 mM, 54% inhibition at 5 mM, 90% at 10 mM | Bacillus subtilis | |
| 3.1.3.8 | Li+ | activates at 1-10 mM, 20% at 2 mM | Bacillus subtilis | |
| 3.1.3.8 | additional information | poor inhibition by Na+ at 1-10 mM | Bacillus subtilis |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.1.3.8 | myo-inositol hexakisphosphate + H2O | Bacillus subtilis | - |
1D-myo-inositol 1,2,4,5,6-pentakisphosphate + phosphate | - |
? | |
| 3.1.3.8 | myo-inositol hexakisphosphate + H2O | Bacillus subtilis B.S.46 | - |
1D-myo-inositol 1,2,4,5,6-pentakisphosphate + phosphate | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.1.3.8 | Bacillus subtilis | - |
isolated from Oryza sativa phyllosphere | - |
| 3.1.3.8 | Bacillus subtilis B.S.46 | - |
isolated from Oryza sativa phyllosphere | - |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.1.3.8 | native enzyme partially by ammonium sulfate fractionation and dialysis | Bacillus subtilis |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 3.1.3.8 | 5.45 | - |
partially purified enzyme, pH 7.0-7.5, 50-55°C | Bacillus subtilis |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.1.3.8 | myo-inositol hexakisphosphate + H2O | - |
Bacillus subtilis | 1D-myo-inositol 1,2,4,5,6-pentakisphosphate + phosphate | - |
? | |
| 3.1.3.8 | myo-inositol hexakisphosphate + H2O | highest activity at 2.05 mM substrate | Bacillus subtilis | 1D-myo-inositol 1,2,4,5,6-pentakisphosphate + phosphate | - |
? | |
| 3.1.3.8 | myo-inositol hexakisphosphate + H2O | - |
Bacillus subtilis B.S.46 | 1D-myo-inositol 1,2,4,5,6-pentakisphosphate + phosphate | - |
? | |
| 3.1.3.8 | myo-inositol hexakisphosphate + H2O | highest activity at 2.05 mM substrate | Bacillus subtilis B.S.46 | 1D-myo-inositol 1,2,4,5,6-pentakisphosphate + phosphate | - |
? | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.1.3.8 | myo-inositol 1,2,3,4,5,6-hexakisphosphate phosphohydrolase | - |
Bacillus subtilis |
| 3.1.3.8 | phytase | - |
Bacillus subtilis |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.1.3.8 | 56.5 | - |
- |
Bacillus subtilis |
Temperature Range [°C]
| EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.1.3.8 | 47 | 68 | 60-73% of maximal activity within this range | Bacillus subtilis |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.1.3.8 | 60 | - |
partially purified enzyme, retains 83% and 60% of its initial activity after 90 and 120 min, respectively | Bacillus subtilis |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.1.3.8 | 7.3 | - |
- |
Bacillus subtilis |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.1.3.8 | 6.3 | 8 | 60-73% of maximal activity within this range | Bacillus subtilis |
pH Stability
| EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.1.3.8 | 3 | 5 | partially purified enzyme, 30 min, complete inactivation | Bacillus subtilis |
| 3.1.3.8 | 6 | 7 | partially purified enzyme, 30 min, completely stable at, 85% activity remains after 24 h | Bacillus subtilis |
| 3.1.3.8 | 8 | 10 | partially purified enzyme, 30 min, 1.16fold activation, completely stable for 24 h | Bacillus subtilis |
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