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Literature summary extracted from

  • Wang, F.; Lai, L.; Liu, Y.; Yang, B.; Wang, Y.
    Expression and characterization of a novel glycerophosphodiester phosphodiesterase from Pyrococcus furiosus DSM 3638 that possesses lysophospholipase D activity (2016), Int. J. Mol. Sci., 17, 831 .
    View publication on PubMedView publication on EuropePMC

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.1.4.46 molecular modeling of structure and docking of substrates. Residues Glu44, Asp46, and Glu109 constitute a metal-binding cleft, with a Mg2+ ion bound Pyrococcus furiosus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.4.46 Co2+ optimum concentration 5 mM Pyrococcus furiosus
3.1.4.46 Mn2+ optimum concentration 5 mM Pyrococcus furiosus
3.1.4.46 additional information divalent cation required, best activators are Co2+ and Mn2+. Poor or no activation by Cu2+, Mg2+, Ca2+, Li+, Fe3+, Zn2+ Pyrococcus furiosus
3.1.4.46 Ni2+ about 40% of the activity with Co2+ or Mn2+ Pyrococcus furiosus

Organic Solvent Stability

EC Number Organic Solvent Comment Organism
3.1.4.46 chloroform 50% v/v, 75% residual activity Pyrococcus furiosus
3.1.4.46 dichloromethane 50% v/v, 95% residual activity Pyrococcus furiosus
3.1.4.46 diethyl ether 50% v/v, 59% residual activity Pyrococcus furiosus
3.1.4.46 Ethyl acetate 50% v/v, 90% residual activity Pyrococcus furiosus
3.1.4.46 hexane 50% v/v, 85% residual activity Pyrococcus furiosus
3.1.4.46 toluene 50% v/v, 85% residual activity Pyrococcus furiosus

Organism

EC Number Organism UniProt Comment Textmining
3.1.4.46 Pyrococcus furiosus Q8TZI9
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.4.46 1-lysophosphatidylcholine + H2O poor substrate in comparison to glycerophosphocholine Pyrococcus furiosus choline + 1-lysophosphatidate
-
 ?
3.1.4.46 2-lysophosphatidylcholine + H2O very poor substrate in comparison to glycerophosphocholine Pyrococcus furiosus choline + 2-lysophosphatidate
-
 ?
3.1.4.46 bis(p-nitrophenyl) phosphate + H2O
-
 Pyrococcus furiosus p-nitrophenyl phosphate + p-nitrophenol
-
 ?
3.1.4.46 glycerophosphocholine + H2O
-
 Pyrococcus furiosus choline + glycerol-3-phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.4.46 ? x * 34059, calculated from sequence Pyrococcus furiosus

Synonyms

EC Number Synonyms Comment Organism
3.1.4.46 Pf2003
-
 Pyrococcus furiosus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.4.46 55
-
-
 Pyrococcus furiosus

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.1.4.46 80
-
 almost complete loss of activity Pyrococcus furiosus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.4.46 50
-
 half-life 17.6 h Pyrococcus furiosus
3.1.4.46 59.6
-
 melting temperature Pyrococcus furiosus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.4.46 8.5
-
-
 Pyrococcus furiosus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.1.4.46 7 10 no activity below pH 7.0 Pyrococcus furiosus