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Literature summary extracted from

  • Ge, L.; Xie, J.; Wu, T.; Zhang, S.; Zhao, L.; Ding, G.; Wang, Z.; Xiao, W.
    Purification and characterisation of a novel alpha-L-rhamnosidase exhibiting transglycosylating activity from Aspergillus oryzae (2017), Int. J. Food Sci. Technol., 52, 2596-2603 .
No PubMed abstract available

Application

EC Number Application Comment Organism
3.2.1.40 food industry the enzyme exhibits transglycosylating activity, which can synthesise rhamnosyl mannitol through the reactions of transglycosylation with inexpensive rhamnose as the glycosyl donor. The enzyme has potential value for glycoside synthesis in the food industry Aspergillus oryzae

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.40 Co2+ 10 mM, 17% loss of activity Aspergillus oryzae
3.2.1.40 Cu2+ 10 mM, 68% loss of activity Aspergillus oryzae
3.2.1.40 Fe2+ 10 mM, 54% loss of activity Aspergillus oryzae
3.2.1.40 Fe3+ 10 mM, 97% loss of activity Aspergillus oryzae
3.2.1.40 Hg2+ 10 mM, 99% loss of activity Aspergillus oryzae
3.2.1.40 K+ 10 mM, 5% loss of activity Aspergillus oryzae
3.2.1.40 Li+ 10 mM, 8% loss of activity Aspergillus oryzae
3.2.1.40 Mn2+ 10 mM, 5% loss of activity Aspergillus oryzae
3.2.1.40 Na+ 10 mM, 10% loss of activity Aspergillus oryzae
3.2.1.40 Ni2+ 10 mM, 41% loss of activity Aspergillus oryzae
3.2.1.40 Zn2+ 10 mM, 13 % loss of activity Aspergillus oryzae

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.40 5.2
-
 4-nitrophenyl alpha-L-rhamnopyranoside pH 4.5, 65°C Aspergillus oryzae

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.40 Ba2+ 10 mM, 1.1fold activation Aspergillus oryzae
3.2.1.40 Ca2+ 10 mM, activation to 105% of control Aspergillus oryzae
3.2.1.40 Mg2+ 10 mM, activation to 104% of control Aspergillus oryzae

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.40 75000
-
 SDS-PAGE Aspergillus oryzae

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.40 Aspergillus oryzae
-
-
-
3.2.1.40 Aspergillus oryzae NL-1
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.40
-
 Aspergillus oryzae

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.40 224.4
-
 pH 4.5, 65°C Aspergillus oryzae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.40 4-nitrophenyl alpha-L-rhamnopyranoside + H2O
-
 Aspergillus oryzae 4-nitrophenol + alpha-L-rhamnopyranose
-
 ?
3.2.1.40 4-nitrophenyl alpha-L-rhamnopyranoside + H2O
-
 Aspergillus oryzae NL-1 4-nitrophenol + alpha-L-rhamnopyranose
-
 ?
3.2.1.40 hesperidin + H2O 240.6% of the activity with rutin Aspergillus oryzae hesperetin 7-O-beta-D-glucoside + alpha-L-rhamnose
-
 ?
3.2.1.40 hesperidin + H2O 240.6% of the activity with rutin Aspergillus oryzae NL-1 hesperetin 7-O-beta-D-glucoside + alpha-L-rhamnose
-
 ?
3.2.1.40 additional information no activity with myricetrin Aspergillus oryzae ?
-
 ?
3.2.1.40 additional information no activity with myricetrin Aspergillus oryzae NL-1 ?
-
 ?
3.2.1.40 naringin + H2O 21% of the activity with rutin Aspergillus oryzae beta-D-glucopyranosyl-2,3-dihydro-4',5,7-trihydroxyflavone + alpha-L-rhamnose
-
 ?
3.2.1.40 naringin + H2O 21% of the activity with rutin Aspergillus oryzae NL-1 beta-D-glucopyranosyl-2,3-dihydro-4',5,7-trihydroxyflavone + alpha-L-rhamnose
-
 ?
3.2.1.40 rutin + H2O
-
 Aspergillus oryzae isoquercitrin + alpha-L-rhamnose
-
 ?
3.2.1.40 rutin + H2O
-
 Aspergillus oryzae NL-1 isoquercitrin + alpha-L-rhamnose
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.40 ? x * 75000, SDS-PAGE Aspergillus oryzae

Synonyms

EC Number Synonyms Comment Organism
3.2.1.40 AoRha
-
 Aspergillus oryzae

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.40 65
-
-
 Aspergillus oryzae

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.2.1.40 45 75 activity is more than 50% of the maximum activity in the temperature range 45°C-75°C Aspergillus oryzae

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.40 65
-
 24 h, residual activity is more than 60% Aspergillus oryzae

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2.1.40 1624
-
 4-nitrophenyl alpha-L-rhamnopyranoside pH 4.5, 65°C Aspergillus oryzae

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.40 4.5
-
-
 Aspergillus oryzae

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.40 3.5 5.5 at pH ranging from 3.5 to 5.5 still the enzyme maintains more than 60% of the optimal pH activity Aspergillus oryzae

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.40 3.5 7 stable in the pH range 3.5-7.0 Aspergillus oryzae

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.2.1.40 312
-
 4-nitrophenyl alpha-L-rhamnopyranoside pH 4.5, 65°C Aspergillus oryzae