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Literature summary extracted from

  • Sharma, S.; Sharma, V.; Nargotra, P.; Bajaj, B.K.
    Process desired functional attributes of an endoxylanase of GH10 family from a new strain of Aspergillus terreus S9 (2018), Int. J. Biol. Macromol., 115, 663-671 .
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.2.1.8 CTAB activity is slightly enhanced Aspergillus terreus
3.2.1.8 Tween-80 activity is slightly enhanced Aspergillus terreus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.8 Co2+ 10 mM, 25% inhibition Aspergillus terreus
3.2.1.8 Cu2+ 10 mM, 66% inhibition Aspergillus terreus
3.2.1.8 EDTA 10 mM, 67% inhibition Aspergillus terreus
3.2.1.8 Fe2+ 10 mM, 14% inhibition Aspergillus terreus
3.2.1.8 Hg2+ 10 mM, 70% inhibition Aspergillus terreus
3.2.1.8 Mg2+ 10 mM, 3% inhibition Aspergillus terreus
3.2.1.8 PMSF 10 mM, 53% inhibition Aspergillus terreus
3.2.1.8 SDS 10 mM, 70% inhibition Aspergillus terreus
3.2.1.8 Zn2+ 10 mM, 40% inhibition Aspergillus terreus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.8 additional information
-
 xylan Km-value: 2.94 mg/ml, pH and temperature not specified in the publication Aspergillus terreus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.8 Ca2+ 10 mM, slight activation Aspergillus terreus
3.2.1.8 K+ 10 mM, slight activation Aspergillus terreus
3.2.1.8 Mn2+ 10 mM, slight activation Aspergillus terreus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.8 33000
-
 SDS-PAGE Aspergillus terreus

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.8 Aspergillus terreus
-
-
-
3.2.1.8 Aspergillus terreus S9
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.8
-
 Aspergillus terreus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.8 additional information no activity against synthetic o- and p-nitrophenyl derivatives of xylose and glucopyranose, carboxylmethylcellulose, locust bean gum, avicel, and laminarin Aspergillus terreus ?
-
 ?
3.2.1.8 additional information no activity against synthetic o- and p-nitrophenyl derivatives of xylose and glucopyranose, carboxylmethylcellulose, locust bean gum, avicel, and laminarin Aspergillus terreus S9 ?
-
 ?
3.2.1.8 xylan + H2O oat spelt xylan and birchwood xylan Aspergillus terreus xylobiose + xylotriose + xylotetraose + xylopentaose + xylohexaose + xyloheptaose + xylooctaose + xylononaose
-
 ?
3.2.1.8 xylan + H2O oat spelt xylan and birchwood xylan Aspergillus terreus S9 xylobiose + xylotriose + xylotetraose + xylopentaose + xylohexaose + xyloheptaose + xylooctaose + xylononaose
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.8 ? x * 33000, SDS-PAGE Aspergillus terreus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2.1.8 1587.28
-
 xylan pH and temperature not specified in the publication Aspergillus terreus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.8 9
-
-
 Aspergillus terreus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.8 6 11 pH 6.0: 70% of maximal activity, pH 11.0: 58.3% of maximal activity Aspergillus terreus

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.8 6 10 90 min, the enzyme retains 75-85% of its initial activity Aspergillus terreus
3.2.1.8 8
-
 90 min, enzyme retains 95.12% of its initial activity Aspergillus terreus
3.2.1.8 11
-
 90 min, enzyme retains 38.75% of its initial activity Aspergillus terreus