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Literature summary extracted from

  • Sapna, C.M.; Singh, B.
    Purification and characterization of a protease-resistant phytase of Aspergillus oryzae SBS50 whose properties make it exceptionally useful as a feed supplement (2017), Int. J. Biol. Macromol., 103, 458-466 .
    View publication on PubMed

Application

EC Number Application Comment Organism
3.1.3.8 agriculture the phytase has the potential to be useful as an animal feed supplement. Among all the feed samples, mustard oil cake is dephytinized more efficiently than other feed samples Aspergillus oryzae

General Stability

EC Number General Stability Organism
3.1.3.8 substrate calcium phytate stablizes the enzyme, while sucrose, glycerol, and starch do not Aspergillus oryzae

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.3.8 2,3-Butanedione inhibits 28% at 1 mM and 52% at 5 mM Aspergillus oryzae
3.1.3.8 2-mercaptoethanol inhibits 20% at 1 mM and 60% at 5 mM Aspergillus oryzae
3.1.3.8 ethylmaleimide inhibits about 10% at 1-5 mM Aspergillus oryzae
3.1.3.8 Fe2+ inhibits 97% at 1 mM and 99% at 5 mM Aspergillus oryzae
3.1.3.8 Fe3+ inhibits 30% at 1 mM and 83% at 5 mM Aspergillus oryzae
3.1.3.8 iodoacetamide inhibits 32% at 1 mM and 36% at 5 mM Aspergillus oryzae
3.1.3.8 isoamyl alcohol inhibits 31% at 2% v/v and 38% at 5% v/v Aspergillus oryzae
3.1.3.8 KI inhibits 31% at 1 M and 44% at 2 M Aspergillus oryzae
3.1.3.8 Mn2+ activates 40% at 1 mM and inhibits 65% at 5 mM Aspergillus oryzae
3.1.3.8 additional information the phytase of Aspergillus oryzae shows protease resistance. Poor effect by urea at 1-2 M and by EDTA at 1-5 mM Aspergillus oryzae
3.1.3.8 SDS inhibits 42% at 0.5% Aspergillus oryzae
3.1.3.8 Sodium molybdate inhibits 54% at 1 mM and 80% at 5 mM Aspergillus oryzae

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.3.8 extracellular
-
Aspergillus oryzae
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.3.8 acetone activates by 35% at 5% v/v Aspergillus oryzae
3.1.3.8 butanol activates by 39% at 5% v/v Aspergillus oryzae
3.1.3.8 Ca2+ activates Aspergillus oryzae
3.1.3.8 Co2+ activates Aspergillus oryzae
3.1.3.8 ethanol activates by 54% at 5% v/v Aspergillus oryzae
3.1.3.8 methanol activates by 54% at 5% v/v Aspergillus oryzae
3.1.3.8 Mg2+ activates Aspergillus oryzae
3.1.3.8 Mn2+ activates 40% at 1 mM and inhibits 65% at 5 mM Aspergillus oryzae
3.1.3.8 NaCl activates at 1 mM by 58%, inhibits at 5 mM by 18% Aspergillus oryzae
3.1.3.8 Triton X-100 activates at 0.5% Aspergillus oryzae
3.1.3.8 Tween 60 activates at 0.1-0.5% Aspergillus oryzae
3.1.3.8 Tween 80 activates at 0.1-0.5% Aspergillus oryzae

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.3.8 65500
-
gel filtration Aspergillus oryzae

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.3.8 myo-inositol hexakisphosphate + H2O Aspergillus oryzae
-
1D-myo-inositol 1,2,4,5,6-pentakisphosphate + phosphate
-
?
3.1.3.8 myo-inositol hexakisphosphate + H2O Aspergillus oryzae SBS50
-
1D-myo-inositol 1,2,4,5,6-pentakisphosphate + phosphate
-
?

Organism

EC Number Organism UniProt Comment Textmining
3.1.3.8 Aspergillus oryzae Q1KNE3 isolated from a soil sample collected from Rohtak, Haryana (India)
-
3.1.3.8 Aspergillus oryzae SBS50 Q1KNE3 isolated from a soil sample collected from Rohtak, Haryana (India)
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.3.8 native extracellular enzyme 10.68fold by ammonium sulfate fractionation, cation exchange chromatography, and gel filtration to homogeneity Aspergillus oryzae

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.3.8 60.63
-
purified enzyme, pH 5.0, 50°C, substrate calcium phytate Aspergillus oryzae

Storage Stability

EC Number Storage Stability Organism
3.1.3.8 4°C, pH 5.0, the purified enzyme retains 80% after 6 months Aspergillus oryzae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.3.8 additional information the enzyme shows a broad substrate specificity with highest activity for calcium phytate, the enzyme is also active with ADP, ATP, glycerol beta-phosphate, glucose 6-phosphate, and 4-nitrophenyl phosphate. Among all the feed samples, mustard oil cake is dephytinized more efficiently than otherfeed samples, e.g. cotton oil cake Aspergillus oryzae ?
-
?
3.1.3.8 additional information the enzyme shows a broad substrate specificity with highest activity for calcium phytate, the enzyme is also active with ADP, ATP, glycerol beta-phosphate, glucose 6-phosphate, and 4-nitrophenyl phosphate. Among all the feed samples, mustard oil cake is dephytinized more efficiently than otherfeed samples, e.g. cotton oil cake Aspergillus oryzae SBS50 ?
-
?
3.1.3.8 myo-inositol hexakisphosphate + H2O
-
Aspergillus oryzae 1D-myo-inositol 1,2,4,5,6-pentakisphosphate + phosphate
-
?
3.1.3.8 myo-inositol hexakisphosphate + H2O best substrate is calcium phytate, sodium phytate results in 60% activity compared to calcium phytate Aspergillus oryzae 1D-myo-inositol 1,2,4,5,6-pentakisphosphate + phosphate
-
?
3.1.3.8 myo-inositol hexakisphosphate + H2O
-
Aspergillus oryzae SBS50 1D-myo-inositol 1,2,4,5,6-pentakisphosphate + phosphate
-
?
3.1.3.8 myo-inositol hexakisphosphate + H2O best substrate is calcium phytate, sodium phytate results in 60% activity compared to calcium phytate Aspergillus oryzae SBS50 1D-myo-inositol 1,2,4,5,6-pentakisphosphate + phosphate
-
?

Subunits

EC Number Subunits Comment Organism
3.1.3.8 dimer 2 * 80000, native PAGE Aspergillus oryzae
3.1.3.8 monomer 1 * 80000, SDS-PAGE Aspergillus oryzae

Synonyms

EC Number Synonyms Comment Organism
3.1.3.8 PhyA
-
Aspergillus oryzae

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.3.8 50
-
-
Aspergillus oryzae

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.1.3.8 30 70 activity range, profile overview Aspergillus oryzae

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.3.8 50
-
purified extracellular enzyme, half-life is 300 min Aspergillus oryzae
3.1.3.8 80
-
purified extracellular enzyme, about 35 min, inactivation Aspergillus oryzae

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.3.8 5
-
-
Aspergillus oryzae

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.1.3.8 3 8 activity range, profile overview Aspergillus oryzae

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.1.3.8 3 5 purified extracellular enzyme, 120 min, 50°C, loss of 20% activity Aspergillus oryzae
3.1.3.8 7
-
purified extracellular enzyme, 120 min, 50°C, loss of 60% activity Aspergillus oryzae