EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
3.1.4.12 | dithiothreitol | - |
Laodelphax striatellus | |
3.1.4.12 | EGTA | stimulates activity at low concentrations | Laodelphax striatellus | |
3.1.4.12 | additional information | phospholipids like cardiolipin, phosphatidic acid, phosphatidylcholine, phosphatidylethanolamine, and phosphatidylserine do not stimulate enzyme activity | Laodelphax striatellus |
EC Number | Cloned (Comment) | Organism |
---|---|---|
3.1.4.12 | expressed in High Five insect cells | Laodelphax striatellus |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
3.1.4.12 | EDTA | significant inhibition at 5 mM | Laodelphax striatellus | |
3.1.4.12 | GW4869 | - |
Laodelphax striatellus | |
3.1.4.12 | Zn2+ | complete inhibition at 0.1 mM | Laodelphax striatellus |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
3.1.4.12 | endoplasmic reticulum | - |
Laodelphax striatellus | 5783 | - |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
3.1.4.12 | Mg2+ | required, high concentrations of up to 50 mM of Mn2+ stimulate enzyme activity | Laodelphax striatellus | |
3.1.4.12 | Mn2+ | required, low concentrations of Mn2+ stimulate enzyme activity | Laodelphax striatellus |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.1.4.12 | sphingomyelin + H2O | Laodelphax striatellus | - |
ceramide + phosphorylcholine | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.1.4.12 | Laodelphax striatellus | A0A2U6TC85 | - |
- |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
3.1.4.12 | head | - |
Laodelphax striatellus | - |
3.1.4.12 | leg | - |
Laodelphax striatellus | - |
3.1.4.12 | midgut | - |
Laodelphax striatellus | - |
3.1.4.12 | ovary | highest expression | Laodelphax striatellus | - |
3.1.4.12 | spermatheca | - |
Laodelphax striatellus | - |
3.1.4.12 | wing | - |
Laodelphax striatellus | - |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.1.4.12 | N-[[6-[(7-nitro-2-1,3-benzoxadiazol-4-yl)amino]hexanoyl]-hexanoyl]-sphingosine-1-phosphocholine + H2O | - |
Laodelphax striatellus | N-[6-[(7-nitro-2-1,3-benzoxadiazol-4-yl)amino]hexanoyl]-D-erythro-sphingosine + phosphorylcholine | - |
? | |
3.1.4.12 | sphingomyelin + H2O | - |
Laodelphax striatellus | ceramide + phosphorylcholine | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.1.4.12 | neutral sphingomyelinase | - |
Laodelphax striatellus |
3.1.4.12 | nSMase | - |
Laodelphax striatellus |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.1.4.12 | 37 | - |
at pH 8.0 | Laodelphax striatellus |
EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.1.4.12 | 27 | 40 | the enzyme shows more than 60% activity between 27 and 40°C, and about 40% activity at 45°C | Laodelphax striatellus |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.1.4.12 | 8 | - |
- |
Laodelphax striatellus |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
3.1.4.12 | 7.5 | - |
the enzyme shows 50% activity at pH 7.5 and 80% activity at pH 9.0 | Laodelphax striatellus |