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Literature summary extracted from

  • Kotani, S.; Izawa, S.; Komai, N.; Takayanagi, A.; Arioka, M.
    Mitochondria-localized phospholipase A2, AoPlaA, in Aspergillus oryzae displays phosphatidylethanolamine-specific activity and is involved in the maintenance of mitochondrial phospholipid composition (2016), Fungal Genet. Biol., 96, 1-11 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.1.4 expression in Escherichia coli Aspergillus oryzae

Protein Variants

EC Number Protein Variants Comment Organism
3.1.1.4 R238A the activity of the R238A mutant isx0140% of that of wild type enzyme. The growth of the strain overexpressing R238A is similar to that of the strain overexpressing wild type AoPlaA, presumably because the residual activity of R238A is sufficient to cause growth retardation Aspergillus oryzae
3.1.1.4 S266A mutation abolishes the enzyme activity Aspergillus oryzae

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.1.4 mitochondrion
-
 Aspergillus oryzae 5739
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.1.4 Ca2+ free Ca2+ enhances the activity of mAoPlaA, but even without the added Ca2+ or in the presence of EGTA to chelate free Ca2+, mAoPlaA displays more than 20% of maximum activity Aspergillus oryzae

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.4 Aspergillus oryzae C7G4B6
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.4
-
 Aspergillus oryzae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.4 1-palmitoyl-2-oleoyl-phosphatidylethanolamine + H2O phosphatidylethanolamine-specific activity. The enzyme displays phospholipases A2 activity to phosphatidylethanolamine, but not to other phospholipids and generates 1-acylated lyso-phosphatidylethanolamine Aspergillus oryzae 1-palmitoyl-lysophosphatidylethanolamine + oleate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.1.1.4 mAoPlaA
-
 Aspergillus oryzae

General Information

EC Number General Information Comment Organism
3.1.1.4 malfunction the AoplaA-disruption strain displays increased content of cardiolipin Aspergillus oryzae
3.1.1.4 metabolism the enzyme is involved in the maintenance of mitochondrial phospholipid composition Aspergillus oryzae