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Literature summary extracted from

  • Nourbakhsh, A.; Collakova, E.; Gillaspy, G.
    Characterization of the inositol monophosphatase gene family in Arabidopsis (2015), Front. Plant Sci., 5, 725 .
    View publication on PubMedView publication on EuropePMC

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.3.15 0.18
-
 L-histidinol 1-phosphate pH 7.5, 25°C Arabidopsis thaliana
3.1.3.25 0.18
-
 D-myo-Inositol 1-phosphate pH 9.0, 25°C Arabidopsis thaliana
3.1.3.25 0.45
-
 D-galactose 1-phosphate pH 9.0, 25°C Arabidopsis thaliana

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.3.15 chloroplast
-
 Arabidopsis thaliana 9507
-
3.1.3.25 chloroplast
-
 Arabidopsis thaliana 9507
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.3.15 Mg2+ assay in presence of 2 mM Arabidopsis thaliana
3.1.3.25 Mg2+ assay in presence of 3 mM Arabidopsis thaliana

Organism

EC Number Organism UniProt Comment Textmining
3.1.3.15 Arabidopsis thaliana Q6NPM8 isoform Impl2, catalyzes both the reactions of EC 3.1.3.15 and 3.1.3.25
-
3.1.3.25 Arabidopsis thaliana Q94F00 isoform Impl1
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.3.25 additional information expression is restricted to above-ground tissues Arabidopsis thaliana
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.3.15 L-histidinol 1-phosphate + H2O 105% of the activity with D-myo-inositol1-phosphate Arabidopsis thaliana L-histidinol + phosphate
-
 ?
3.1.3.25 D-galactose 1-phosphate + H2O 105% of the activity with D-myo-inositol 1-phosphate Arabidopsis thaliana D-galactose + phosphate
-
 ?
3.1.3.25 D-myo-inositol 1-phosphate + H2O
-
 Arabidopsis thaliana D-myo-inositol + phosphate
-
 ?
3.1.3.25 D-myo-inositol 2-phosphate + H2O 18.8% of the activity with D-myo-inositol 1-phosphate Arabidopsis thaliana D-myo-inositol + phosphate
-
 ?
3.1.3.25 D-myo-inositol 3-phosphate + H2O 17.8% of the activity with D-myo-inositol 1-phosphate Arabidopsis thaliana D-myo-inositol + phosphate
-
 ?
3.1.3.25 glycerol 2-phosphate + H2O 39.7% of the activity with D-myo-inositol 1-phosphate Arabidopsis thaliana glycerol + phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.3.15 ? x * 60000, SDS-PAGE, recombinant GST-fusion protein Arabidopsis thaliana
3.1.3.25 ? x * 65000, SDS-PAGE, recombinant GST-fusion protein Arabidopsis thaliana

Synonyms

EC Number Synonyms Comment Organism
3.1.3.15 At4g39120
-
 Arabidopsis thaliana
3.1.3.15 HisN7
-
 Arabidopsis thaliana
3.1.3.15 IMPL2
-
 Arabidopsis thaliana
3.1.3.25 Impl1
-
 Arabidopsis thaliana

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.1.3.15 1.3
-
 L-histidinol 1-phosphate pH 7.5, 25°C Arabidopsis thaliana
3.1.3.25 0.6
-
 D-myo-Inositol 1-phosphate pH 9.0, 25°C Arabidopsis thaliana
3.1.3.25 2.4
-
 D-galactose 1-phosphate pH 9.0, 25°C Arabidopsis thaliana

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.3.15 7.5
-
-
 Arabidopsis thaliana
3.1.3.25 9
-
-
 Arabidopsis thaliana

General Information

EC Number General Information Comment Organism
3.1.3.25 physiological function gene expression is essential for viability Arabidopsis thaliana

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.1.3.15 7.9
-
 L-histidinol 1-phosphate pH 7.5, 25°C Arabidopsis thaliana
3.1.3.25 3.3
-
 D-myo-Inositol 1-phosphate pH 9.0, 25°C Arabidopsis thaliana
3.1.3.25 5.3
-
 D-galactose 1-phosphate pH 9.0, 25°C Arabidopsis thaliana