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Literature summary extracted from

  • Gao, X.; Wu, J.; Wu, D.
    Rational design of the beta-galactosidase from Aspergillus oryzae to improve galactooligosaccharide production (2019), Food Chem., 286, 362-367 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.23 expressed in Escherichia coli JM109 cells Aspergillus oryzae

Protein Variants

EC Number Protein Variants Comment Organism
3.2.1.23 N140C the mutant shows improved activity compared to the wild type enzyme Aspergillus oryzae
3.2.1.23 N140C/W806F the mutant shows improved activity compared to the wild type enzyme Aspergillus oryzae
3.2.1.23 W806F the mutant shows improved activity compared to the wild type enzyme Aspergillus oryzae

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.23 0.0381
-
 2-nitrophenyl beta-D-galactoside mutant enzyme W806F, at pH 5.0 and 60°C Aspergillus oryzae
3.2.1.23 0.0522
-
 2-nitrophenyl beta-D-galactoside wild type enzyme, at pH 5.0 and 60°C Aspergillus oryzae

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.23 lactose + H2O Aspergillus oryzae
-
 beta-D-glucose + beta-D-galactose
-
 ?
3.2.1.23 lactose + H2O Aspergillus oryzae ATCC 42149
-
 beta-D-glucose + beta-D-galactose
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.23 Aspergillus oryzae Q2UCU3
-
-
3.2.1.23 Aspergillus oryzae ATCC 42149 Q2UCU3
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.23 2-nitrophenyl beta-D-galactoside + H2O
-
 Aspergillus oryzae 2-nitrophenol + beta-D-galactose
-
 ?
3.2.1.23 2-nitrophenyl beta-D-galactoside + H2O
-
 Aspergillus oryzae ATCC 42149 2-nitrophenol + beta-D-galactose
-
 ?
3.2.1.23 lactose + H2O
-
 Aspergillus oryzae beta-D-glucose + beta-D-galactose
-
 ?
3.2.1.23 lactose + H2O
-
 Aspergillus oryzae ATCC 42149 beta-D-glucose + beta-D-galactose
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.2.1.23 beta-gal
-
 Aspergillus oryzae

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.23 60
-
-
 Aspergillus oryzae

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2.1.23 179.5
-
 2-nitrophenyl beta-D-galactoside mutant enzyme W806F, at pH 5.0 and 60°C Aspergillus oryzae
3.2.1.23 224.9
-
 2-nitrophenyl beta-D-galactoside wild type enzyme, at pH 5.0 and 60°C Aspergillus oryzae

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.23 5
-
-
 Aspergillus oryzae

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.2.1.23 4333
-
 2-nitrophenyl beta-D-galactoside wild type enzyme, at pH 5.0 and 60°C Aspergillus oryzae
3.2.1.23 4666
-
 2-nitrophenyl beta-D-galactoside mutant enzyme W806F, at pH 5.0 and 60°C Aspergillus oryzae