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Literature summary extracted from

  • Khalili Ghadikolaei, K.; Gharechahi, J.; Haghbeen, K.; Akbari Noghabi, K.; Hosseini Salekdeh, G.; Shahbani Zahiri, H.
    A cold-adapted endoglucanase from camel rumen with high catalytic activity at moderate and low temperatures an anomaly of truly cold-adapted evolution in a mesophilic environment (2018), Extremophiles, 22, 315-326 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.4 cloned from camel rumen metagenom, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3), subcloning in Escherichia coli strain DH5alpha uncultured bacterium

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.4 acetone
-
 uncultured bacterium
3.2.1.4 Benzene
-
 uncultured bacterium
3.2.1.4 chloroform
-
 uncultured bacterium
3.2.1.4 Cyclohexane
-
 uncultured bacterium
3.2.1.4 ethanol
-
 uncultured bacterium
3.2.1.4 guanidinium hydrochloride 72.2% at 3% uncultured bacterium
3.2.1.4 isobutanol
-
 uncultured bacterium
3.2.1.4 Isopropanol
-
 uncultured bacterium
3.2.1.4 methanol
-
 uncultured bacterium
3.2.1.4 additional information organic solvents at 10-20% uncultured bacterium
3.2.1.4 n-hexane
-
 uncultured bacterium
3.2.1.4 PMSF 46.7% at 5 mM uncultured bacterium
3.2.1.4 SDS complete inhibition at 0.1% uncultured bacterium
3.2.1.4 Toluene
-
 uncultured bacterium
3.2.1.4 Tween 20 31% inhibition at 20% uncultured bacterium
3.2.1.4 Urea 82.3% inhibition at 10%, 42.3% at 7%, and 24.3 at 5% uncultured bacterium
3.2.1.4 Zn2+ about 60-80% inhibition at 5-10 mM uncultured bacterium

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.4 additional information
-
 additional information Vmax and Km are 339 U/mg and 2.57 mg/ml, respectively, Michaelis-Menten kinetics and thermodynamic analysis, overview uncultured bacterium

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.4 Cd2+ activates slightly at 10 mM uncultured bacterium
3.2.1.4 Co2+ activates at 5-10 mM uncultured bacterium
3.2.1.4 Mn2+ activates at 5-10 mM uncultured bacterium
3.2.1.4 Na+ activates at 5 mM, not at 10 mM uncultured bacterium

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.4 uncultured bacterium A0A1W5YNV1 isolated from camel rumen
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.4 recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography uncultured bacterium

Source Tissue

EC Number Source Tissue Comment Organism Textmining

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.4 carboxymethyl cellulose + H2O
-
 uncultured bacterium ?
-
 ?
3.2.1.4 additional information no activity with starch uncultured bacterium ?
-
 ?
3.2.1.4 oat spelt xylan + H2O
-
 uncultured bacterium ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.4 ? x * 46500, about, sequence calculation uncultured bacterium

Synonyms

EC Number Synonyms Comment Organism
3.2.1.4 CelCM3
-
 uncultured bacterium
3.2.1.4 cellulase CM3
-
 uncultured bacterium
3.2.1.4 endoglucanase
-
 uncultured bacterium

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.4 30
-
-
 uncultured bacterium

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.2.1.4 10 60 optimum temperature is 30°C, over 60% of maximal activity in the range of 10-50°C, 13% at 60°C, no activity at 70-80°C, profile overview uncultured bacterium

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.4 4 45 the enzyme has an estimated low melting temperature of 45°C, about 50% activity remaining at 4°C, it is identified to be cold-adapted uncultured bacterium

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.4 5
-
-
 uncultured bacterium

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.4 3 9 activity range, over 50% of maximal activity at pH 4.0-7.5, profile overview uncultured bacterium