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Literature summary extracted from

  • Jang, W.J.; Lee, J.M.; Park, H.D.; Choi, Y.B.; Kong, I.S.
    N-terminal domain of the beta-propeller phytase of Pseudomonas sp. FB15 plays a role for retention of low-temperature activity and catalytic efficiency (2018), Enzyme Microb. Technol., 117, 84-90 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.3.8 DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic tree, recombinant expression of the His-tagged full-length enzyme and its N- and C-terminal domains in Escherichia coli strain BL21(DE3), subcloning in Escherichia coli strain DH5alpha Pseudomonas sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.3.8 additional information
-
 additional information kinetics and thermodynamics of recombinant full-length enzyme and C-terminal domain, overview Pseudomonas sp.
3.1.3.8 0.856
-
 myo-inositol hexakisphosphate pH 6.0, 40°C, recombinant His-tagged C-terminal domain Pseudomonas sp.
3.1.3.8 0.858
-
 myo-inositol hexakisphosphate pH 6.0, 40°C, recombinant His-tagged full-length enzyme Pseudomonas sp.

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.3.8 CaCl2 CaCl2 is required for activity, and the optimal concentration of CaCl2 for the full-length enzyme is 4 mM, the isolated C-terminal domain is most active at 3 mM Pseudomonas sp.

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.3.8 myo-inositol hexakisphosphate + H2O Pseudomonas sp.
-
 1D-myo-inositol 1,2,4,5,6-pentakisphosphate + phosphate
-
 ?
3.1.3.8 myo-inositol hexakisphosphate + H2O Pseudomonas sp. FB15
-
 1D-myo-inositol 1,2,4,5,6-pentakisphosphate + phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.3.8 Pseudomonas sp. A0A1S6WME7 isolated from foreshore soil on the coast of Busan, Korea
-
3.1.3.8 Pseudomonas sp. FB15 A0A1S6WME7 isolated from foreshore soil on the coast of Busan, Korea
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.3.8 recombinant His-tagged full-length enzyme and its N- and C-terminal domains from Escherichia coli strain BL21(DE3) by nickel affinity chromatography Pseudomonas sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.3.8 additional information the C-terminal domain is catalytically active, while the N-terminal domain exhibits no measurable myo-inositol hexakisphosphate phosphohydrolase activity Pseudomonas sp. ?
-
 ?
3.1.3.8 additional information the C-terminal domain is catalytically active, while the N-terminal domain exhibits no measurable myo-inositol hexakisphosphate phosphohydrolase activity Pseudomonas sp. FB15 ?
-
 ?
3.1.3.8 myo-inositol hexakisphosphate + H2O
-
 Pseudomonas sp. 1D-myo-inositol 1,2,4,5,6-pentakisphosphate + phosphate
-
 ?
3.1.3.8 myo-inositol hexakisphosphate + H2O
-
 Pseudomonas sp. FB15 1D-myo-inositol 1,2,4,5,6-pentakisphosphate + phosphate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.1.3.8 beta-propeller phytase
-
 Pseudomonas sp.
3.1.3.8 myo-inositol hexakisphosphate phosphohydrolase
-
 Pseudomonas sp.
3.1.3.8 phytase
-
 Pseudomonas sp.
3.1.3.8 PSphy
-
 Pseudomonas sp.

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.3.8 40
-
-
 Pseudomonas sp.

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.3.8 25 40 purified recombinant enzyme, over 80% activity remaining within this range, thermal stability of recobinant enzyme PSphy decreases sharply above 40°C in the presence of CaCl2, whereas it remains stable up to 60°C in its absence Pseudomonas sp.

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.1.3.8 1.67
-
 myo-inositol hexakisphosphate pH 6.0, 40°C, recombinant His-tagged C-terminal domain Pseudomonas sp.
3.1.3.8 20.39
-
 myo-inositol hexakisphosphate pH 6.0, 40°C, recombinant His-tagged full-length enzyme Pseudomonas sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.3.8 6
-
-
 Pseudomonas sp.

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.1.3.8 4 10 purified recombinant enzyme, over 60% activity remaining within this range Pseudomonas sp.
3.1.3.8 8
-
 purified recombinant enzyme, completely stable at Pseudomonas sp.

General Information

EC Number General Information Comment Organism
3.1.3.8 malfunction activity and catalytic efficiency at low temperature are reduced in a recombinant protein from which the N-terminal domain is removed Pseudomonas sp.
3.1.3.8 additional information the N-terminal domain is required for the low temperature activity and high catalytic efficiency of PSphy Pseudomonas sp.

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.1.3.8 1.95
-
 myo-inositol hexakisphosphate pH 6.0, 40°C, recombinant His-tagged C-terminal domain Pseudomonas sp.
3.1.3.8 23.76
-
 myo-inositol hexakisphosphate pH 6.0, 40°C, recombinant His-tagged full-length enzyme Pseudomonas sp.