EC Number | Cloned (Comment) | Organism |
---|---|---|
3.1.3.8 | DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic tree, recombinant expression of the His-tagged full-length enzyme and its N- and C-terminal domains in Escherichia coli strain BL21(DE3), subcloning in Escherichia coli strain DH5alpha | Pseudomonas sp. |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.1.3.8 | additional information | - |
additional information | kinetics and thermodynamics of recombinant full-length enzyme and C-terminal domain, overview | Pseudomonas sp. | |
3.1.3.8 | 0.856 | - |
myo-inositol hexakisphosphate | pH 6.0, 40°C, recombinant His-tagged C-terminal domain | Pseudomonas sp. | |
3.1.3.8 | 0.858 | - |
myo-inositol hexakisphosphate | pH 6.0, 40°C, recombinant His-tagged full-length enzyme | Pseudomonas sp. |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
3.1.3.8 | CaCl2 | CaCl2 is required for activity, and the optimal concentration of CaCl2 for the full-length enzyme is 4 mM, the isolated C-terminal domain is most active at 3 mM | Pseudomonas sp. |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.1.3.8 | myo-inositol hexakisphosphate + H2O | Pseudomonas sp. | - |
1D-myo-inositol 1,2,4,5,6-pentakisphosphate + phosphate | - |
? | |
3.1.3.8 | myo-inositol hexakisphosphate + H2O | Pseudomonas sp. FB15 | - |
1D-myo-inositol 1,2,4,5,6-pentakisphosphate + phosphate | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.1.3.8 | Pseudomonas sp. | A0A1S6WME7 | isolated from foreshore soil on the coast of Busan, Korea | - |
3.1.3.8 | Pseudomonas sp. FB15 | A0A1S6WME7 | isolated from foreshore soil on the coast of Busan, Korea | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.1.3.8 | recombinant His-tagged full-length enzyme and its N- and C-terminal domains from Escherichia coli strain BL21(DE3) by nickel affinity chromatography | Pseudomonas sp. |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.1.3.8 | additional information | the C-terminal domain is catalytically active, while the N-terminal domain exhibits no measurable myo-inositol hexakisphosphate phosphohydrolase activity | Pseudomonas sp. | ? | - |
? | |
3.1.3.8 | additional information | the C-terminal domain is catalytically active, while the N-terminal domain exhibits no measurable myo-inositol hexakisphosphate phosphohydrolase activity | Pseudomonas sp. FB15 | ? | - |
? | |
3.1.3.8 | myo-inositol hexakisphosphate + H2O | - |
Pseudomonas sp. | 1D-myo-inositol 1,2,4,5,6-pentakisphosphate + phosphate | - |
? | |
3.1.3.8 | myo-inositol hexakisphosphate + H2O | - |
Pseudomonas sp. FB15 | 1D-myo-inositol 1,2,4,5,6-pentakisphosphate + phosphate | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.1.3.8 | beta-propeller phytase | - |
Pseudomonas sp. |
3.1.3.8 | myo-inositol hexakisphosphate phosphohydrolase | - |
Pseudomonas sp. |
3.1.3.8 | phytase | - |
Pseudomonas sp. |
3.1.3.8 | PSphy | - |
Pseudomonas sp. |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.1.3.8 | 40 | - |
- |
Pseudomonas sp. |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.1.3.8 | 25 | 40 | purified recombinant enzyme, over 80% activity remaining within this range, thermal stability of recobinant enzyme PSphy decreases sharply above 40°C in the presence of CaCl2, whereas it remains stable up to 60°C in its absence | Pseudomonas sp. |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.1.3.8 | 1.67 | - |
myo-inositol hexakisphosphate | pH 6.0, 40°C, recombinant His-tagged C-terminal domain | Pseudomonas sp. | |
3.1.3.8 | 20.39 | - |
myo-inositol hexakisphosphate | pH 6.0, 40°C, recombinant His-tagged full-length enzyme | Pseudomonas sp. |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.1.3.8 | 6 | - |
- |
Pseudomonas sp. |
EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|---|
3.1.3.8 | 4 | 10 | purified recombinant enzyme, over 60% activity remaining within this range | Pseudomonas sp. |
3.1.3.8 | 8 | - |
purified recombinant enzyme, completely stable at | Pseudomonas sp. |
EC Number | General Information | Comment | Organism |
---|---|---|---|
3.1.3.8 | malfunction | activity and catalytic efficiency at low temperature are reduced in a recombinant protein from which the N-terminal domain is removed | Pseudomonas sp. |
3.1.3.8 | additional information | the N-terminal domain is required for the low temperature activity and high catalytic efficiency of PSphy | Pseudomonas sp. |
EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.1.3.8 | 1.95 | - |
myo-inositol hexakisphosphate | pH 6.0, 40°C, recombinant His-tagged C-terminal domain | Pseudomonas sp. | |
3.1.3.8 | 23.76 | - |
myo-inositol hexakisphosphate | pH 6.0, 40°C, recombinant His-tagged full-length enzyme | Pseudomonas sp. |