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Literature summary extracted from

  • Shi, R.; Mullins, E.A.; Shen, X.X.; Lay, K.T.; Yuen, P.K.; David, S.S.; Rokas, A.; Eichman, B.F.
    Selective base excision repair of DNA damage by the non-base-flipping DNA glycosylase AlkC (2018), EMBO J., 37, 63-74 .
    View publication on PubMedView publication on EuropePMC

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.2.2.20 structure resembling a catalytic intermediate complex. AlkC uses unique HEAT-like repeat folds and immunoglobulin-like domains to induce a sharp kink in the DNA, exposing the damaged nucleobase to active site residues that project into the DNA. The active site can accommodate and excise N3-methylcytosine and N1-methyladenine Pseudomonas fluorescens
3.2.2.20 structure resembling a catalytic intermediate complex. AlkC uses unique HEAT-like repeat folds and immunoglobulin-like domains to induce a sharp kink in the DNA, exposing the damaged nucleobase to active site residues that project into the DNA. The active site can accommodate and excise N3-methylcytosine and N1-methyladenine Bacillus cereus

Protein Variants

EC Number Protein Variants Comment Organism
3.2.2.20 E121A about 20% of wild-type activity Pseudomonas fluorescens
3.2.2.20 E156A about 50% of wild-type activity Pseudomonas fluorescens
3.2.2.20 W164A activity similar to wild-type Pseudomonas fluorescens

Organism

EC Number Organism UniProt Comment Textmining
3.2.2.20 Bacillus cereus
-
-
-
3.2.2.20 Pseudomonas fluorescens
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.2.20 alkylated DNA + H2O
-
Pseudomonas fluorescens 3-methyladenine + ?
-
?
3.2.2.20 alkylated DNA + H2O
-
Bacillus cereus 3-methyladenine + ?
-
?
3.2.2.20 alkylated DNA + H2O
-
Pseudomonas fluorescens 1-methyladenine + ?
-
?
3.2.2.20 alkylated DNA + H2O
-
Bacillus cereus 1-methyladenine + ?
-
?
3.2.2.20 alkylated DNA + H2O
-
Pseudomonas fluorescens 3-methylcytosine + ?
-
?
3.2.2.20 alkylated DNA + H2O
-
Bacillus cereus 3-methylcytosine + ?
-
?
3.2.2.20 additional information AlkC exhibits robust activity for 3-methylcytosine and modest activity for 1-methyladenosine Pseudomonas fluorescens ?
-
?
3.2.2.20 additional information AlkC exhibits robust activity for 3-methylcytosine and modest activity for 1-methyladenosine Bacillus cereus ?
-
?

Synonyms

EC Number Synonyms Comment Organism
3.2.2.20 AlkC
-
Pseudomonas fluorescens
3.2.2.20 AlkC
-
Bacillus cereus