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Literature summary extracted from

  • Unsleber, S.; Borisova, M.; Mayer, C.
    Enzymatic synthesis and semi-preparative isolation of N-acetylmuramic acid 6-phosphate (2017), Carbohydr. Res., 445, 98-103 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.3.105 expressed in Escherichia coli BL21(DE3) cells Pseudomonas putida

General Stability

EC Number General Stability Organism
3.1.3.105 the long-term stability of the enzyme in solution at -80°C is increased by the addition of 20% (v/v) glycerol Pseudomonas putida

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.3.105 0.31
-
 N-acetyl-D-muramate 6-phosphate at pH 7.6 and 22°C Pseudomonas putida

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.3.105 Mg2+ 10 mM used in assay conditions Pseudomonas putida

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.3.105 N-acetyl-D-muramate 6-phosphate + H2O Pseudomonas putida
-
 N-acetyl-D-muramate + phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.3.105 Pseudomonas putida Q88M11
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.3.105 Ni-NTA column chromatography and Superdex 200 gel filtration Pseudomonas putida

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.3.105 additional information neither alpha-1-phosphorylated N-acetyl-D-muramate, N-acetyl-D-glucose, or D-glucose nor glucosamine 6-phosphate or D-glucose 6-phosphate is used as a substrate Pseudomonas putida ?
-
 ?
3.1.3.105 N-acetyl-D-glucosamine 6-phosphate + H2O
-
 Pseudomonas putida N-acetyl-D-glucosamine + phosphate
-
 ?
3.1.3.105 N-acetyl-D-muramate 6-phosphate + H2O
-
 Pseudomonas putida N-acetyl-D-muramate + phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.3.105 ? x * 25890, calculated from amino acid sequence Pseudomonas putida

Synonyms

EC Number Synonyms Comment Organism
3.1.3.105 mupP
-
 Pseudomonas putida
3.1.3.105 MurNAc 6P phosphatase
-
 Pseudomonas putida
3.1.3.105 N-acetylmuramic acid 6-phosphate phosphatase
-
 Pseudomonas putida

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.3.105 45
-
-
 Pseudomonas putida

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.3.105 22 45 the recombinant enzyme loses about 10% of its activity at 22°C and 70% at 37°C within 40 min and is completely inactivated within 40 min at 45°C. About 50% of its activity remain at 22°C, and about 85% of its activity remain at 37°C Pseudomonas putida

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.1.3.105 0.88
-
 N-acetyl-D-muramate 6-phosphate at pH 7.6 and 22°C Pseudomonas putida

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.3.105 6 9
-
 Pseudomonas putida

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.1.3.105 4 10 at pH 4.0 and pH 10.0, the enzyme activity is 40 and 60%, respectively, of the maximum Pseudomonas putida

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.1.3.105 2.84
-
 N-acetyl-D-muramate 6-phosphate at pH 7.6 and 22°C Pseudomonas putida