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Literature summary extracted from

  • Gissibl, A.; Care, A.; Parker, L.M.; Iqbal, S.; Hobba, G; Nevalainen, H.; Sunna, A.
    Microwave pretreatment of paramylon enhances the enzymatic production of soluble beta-1,3-glucans with immunostimulatory activity (2018), Carbohydr. Polym., 196, 339-347 .
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.2.1.39 additional information hydrothermal microwave pretreatment of substrate paramylon at 170°C for 2 min is established to facilitate and enhance the enzymatic production of soluble bioactive beta-1,3-glucans from the recalcitrant substrate paramylon, structure comparisons of paramylon granules with and without heat treatment, overview. Virtual absence of enzymatic hydrolysis of paramylon pretreated at the lower incubation temperatures may be explained by steric hindrance of the enzymes Helix pomatia

Application

EC Number Application Comment Organism
3.2.1.39 pharmacology action of endo-beta-1,3-glucanase on the microwave-pretreated paramylon produces soluble beta-1,3-glucans with degrees of polymerisation (DP) ranging from 2-59. The hydrolysis products induce TNFalpha production exhibiting an immunostimulatory effect on murine J774 macrophages Helix pomatia

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.39 paramylon + H2O Helix pomatia
-
 ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.39 Helix pomatia
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.39 additional information hydrothermal microwave pretreatment of substrate paramylon at 170 °C for 2 min is established to facilitate the enzymatic production of soluble bioactive beta-1,3-glucans from the recalcitrant substrate paramylon, structure comparisons of paramylon granules with and without heat treatment, overview. Virtual absence of enzymatic hydrolysis of paramylon pretreated at the lower incubation temperatures may be explained by steric hindrance of the enzymes Helix pomatia ?
-
 ?
3.2.1.39 paramylon + H2O
-
 Helix pomatia ?
-
 ?
3.2.1.39 paramylon + H2O action of endo-beta-1,3-glucanase on the microwave-pretreated paramylon produces soluble beta-1,3-glucans with degrees of polymerisation (DP) ranging from 2-59 Helix pomatia ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.2.1.39 beta-1,3-D-glucanase
-
 Helix pomatia
3.2.1.39 endo-beta-1,3-glucanase
-
 Helix pomatia

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.39 37
-
 assay at Helix pomatia

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.39 5
-
 assay at Helix pomatia