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Literature summary extracted from

  • Yang, M.; Teymorian, S.; Olivares, P.; Murthy, P.
    Extracellular expression of alkaline phytase in Pichia pastoris Influence of signal peptides, promoters and growth medium (2015), Biotechnol. Rep., 6, 112-118 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.3.72 recombinant extracellular expression of Lilium longiflorum alkaline phytase in Pichia pastoris strain X33. For analysis of the influence of the native signal sequences on alpha-MF-directed secretion, three expression constructs are generated by inserting three forms of rLlAlp2 downstream of the alpha-MF secretion signal in pPICZaAvectors: 1. full length optimized rLlAlp2 sequence (pPICZaA-op-rLlAlp2), 2. truncated oprLlAlp2 without QKTEL at the C-terminus (pPICZaA-op-LlAlp2DC), and 3. truncated op-rLlAlp2 without the first 19 aa at the N-terminus and without QKTEL at the C-terminus (pPICZaA-oprLlAlp2DNDC). By switching from the strong, tightly regulated promoter PAOX1 to the weaker, constitutive promoter PGAP and optimizing culture conditions, an 8 to 10fold increase in extracellular expression of rLlALP2 is achieved, optimization of culture conditions for enhanced expression, method evaluation, overview Lilium longiflorum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.3.72 myo-inositol hexakisphosphate + H2O Lilium longiflorum phosphate cleavage position is not determined myo-inositol pentakisphosphate + phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.3.72 Lilium longiflorum Q0GYS2
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.3.72 pollen
-
 Lilium longiflorum
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.3.72 myo-inositol hexakisphosphate + H2O phosphate cleavage position is not determined Lilium longiflorum myo-inositol pentakisphosphate + phosphate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.1.3.72 alkaline phytase
-
 Lilium longiflorum
3.1.3.72 LlALP2
-
 Lilium longiflorum

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.3.72 37
-
 assay at Lilium longiflorum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.3.72 8
-
 assay at Lilium longiflorum