Literature summary extracted from

Damnjanovic, J.; Kuroiwa, C.; Tanaka, H.; Ishida, K.; Nakano, H.; Iwasaki, Y.
Directing positional specificity in enzymatic synthesis of bioactive 1-phosphatidylinositol by protein engineering of a phospholipase D (2016), Biotechnol. Bioeng., 113, 62-71 .

Protein Variants

EC Number	Protein Variants	Comment	Organism
3.1.4.4	W187N/Y191Y/Y385R	mutant generates phosphatidylinositol as a mixture of 1-phosphatidylinositol and 3-phosphatidylinositol in the ratio of 76/24	Streptomyces antibioticus
3.1.4.4	W187N/Y191Y/Y385R/G186T	mutant generates phosphatidylinositol as a mixture of 1-phosphatidylinositol and 3-phosphatidylinositol in the ratio of 87/13 and of 97/3 at 20°C, respectively	Streptomyces antibioticus
3.1.4.4	W187N/Y191Y/Y385R/G186T	mutant generates phosphatidylinositol as a mixture of 1-phosphatidylinositol and 3-phosphatidylinositol in the ratio of 93/7 at 37°C and of 97/3 at 20°C, respectively	Streptomyces antibioticus

Organism

EC Number	Organism	UniProt	Comment	Textmining
3.1.4.4	Streptomyces antibioticus	Q53728	-	-

Substrates and Products (Substrate)

EC Number	Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
3.1.4.4	1,2-dioleoylphosphatidylcholine + inositol	-	Streptomyces antibioticus	1,2-dioleoylphosphatidylinositol + choline	-	?

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Release 2023.1 (January 2023)