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Literature summary extracted from

  • Wei, R.; Oeser, T.; Schmidt, J.; Meier, R.; Barth, M.; Then, J.; Zimmermann, W.
    Engineered bacterial polyester hydrolases efficiently degrade polyethylene terephthalate due to relieved product inhibition (2016), Biotechnol. Bioeng., 113, 1658-1665 .
    View publication on PubMed

Protein Variants

EC Number Protein Variants Comment Organism
3.1.1.101 G62A mutant enzyme shows highly increased activity in hydrolyzing PET films and fibers. The mutant causes a weight loss of PET films of more than 42% after 50 h of hydrolysis, corresponding to a 2.7fold increase compared to the wild type enzyme. Kinetic analysis based on the released PET hydrolysis products confirms the superior hydrolytic activity of G62A with a fourfold higher hydrolysis rate constant and a 1.5fold lower substrate binding constant than those of the wild type enzyme. Mutant enzyme G62A reveals a 5.5fold lower binding constant to the inhibitor than the wild type enzyme indicating that its increased PET hydrolysis activity is the result of a relieved product inhibition by mono-(2-hydroxyethyl) terephthalate Thermobifida fusca
3.1.1.101 G62A/I213S mutant enzyme shows highly increased activity in hydrolyzing PET films and fibers. The mutant causes a weight loss of PET films of more than 42% after 50 h of hydrolysis, corresponding to a 2.7fold increase compared to the wild type enzyme Thermobifida fusca

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.1.101 mono-(2-hydroxyethyl) terephthalate strong inhibitor Thermobifida fusca

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.101 Thermobifida fusca E5BBQ3
-
-
3.1.1.101 Thermobifida fusca KW3 E5BBQ3
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.101 (ethylene terephthalate)n + H2O
-
 Thermobifida fusca (ethylene terephthalate)n-1 + 4-[(2-hydroxyethoxy)carbonyl]benzoate
-
 ?
3.1.1.101 (ethylene terephthalate)n + H2O
-
 Thermobifida fusca KW3 (ethylene terephthalate)n-1 + 4-[(2-hydroxyethoxy)carbonyl]benzoate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.1.1.101 TfCut2
-
 Thermobifida fusca