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Literature summary extracted from

  • Millar, R.; Rahmanpour, R.; Yuan, E.W.J.; White, C.; Bugg, T.D.H.
    Esterase EstK from Pseudomonas putida mt-2 An enantioselective acetylesterase with activity for deacetylation of xylan and poly(vinylacetate) (2017), Biotechnol. Appl. Biochem., 64, 803-809 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.1.6 expressed in Escherichia coli BL21(DE3) cells Pseudomonas putida

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.1.6 0.285
-
 4-nitrophenyl acetate at pH 8.0 and 20°C Pseudomonas putida

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.1.6 beechwood xylan + H2O Pseudomonas putida
-
 ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.6 Pseudomonas putida
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.6 Sepharose Ni-NTA column chromatography Pseudomonas putida

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.6 4-acetoxybenzoic acid + H2O
-
 Pseudomonas putida 4-hydroxybenzoate + acetate
-
 ?
3.1.1.6 4-nitrophenyl acetate + H2O
-
 Pseudomonas putida 4-nitrophenol + acetate
-
 ?
3.1.1.6 beechwood xylan + H2O
-
 Pseudomonas putida ?
-
 ?
3.1.1.6 additional information the enzyme has no activity toward 4-nitrophenyl valerate, 4-nitrophenyl caproate, 4-nitrophenyl palmitate, 4-nitrophenyl benzoate, 4-nitrophenyl phosphate, methyl benzoate, methyl 4-hydroxybenzoate, or methyl cyclohexane-1-carboxylate Pseudomonas putida ?
-
 ?
3.1.1.6 poly(vinylacetate) + H2O
-
 Pseudomonas putida ? + acetate
-
 ?
3.1.1.6 racemic 1-phenylethyl acetate + H2O
-
 Pseudomonas putida (1R)-phenylethanol + (1S)-phenylethanol + acetate
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.1.6 ? x * 36000, SDS-PAGE Pseudomonas putida
3.1.1.6 ? x * 36300, calculated from amino acid sequence Pseudomonas putida

Synonyms

EC Number Synonyms Comment Organism
3.1.1.6 ESTK
-
 Pseudomonas putida

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.1.1.6 5.5
-
 4-nitrophenyl acetate at pH 8.0 and 20°C Pseudomonas putida

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.1.6 7.5 8.5
-
 Pseudomonas putida