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Literature summary extracted from
- Enzymatic characterization of an extracellular glucoamylase from Tricholoma matsutake and its cloning and secretory expression in Pichia pastoris (2018), Biosci. Biotechnol. Biochem., 82, 2180-2190 .
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 3.2.1.3 | gene TmGlu1, DNA and amino acid sequeence determination and analysis, recombinant secretory expression of the enzyme in Pichia pastoris strain X-33 | Tricholoma matsutake |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.2.1.3 | Ag+ | complete inhibition | Tricholoma matsutake |  |
| 3.2.1.3 | Cu2+ | 14% inhibition at 5 mM | Tricholoma matsutake | |
| 3.2.1.3 | Fe2+ | 48% inhibition at 5 mM | Tricholoma matsutake | |
| 3.2.1.3 | Hg2+ | 52% inhibition at 5 mM | Tricholoma matsutake | |
| 3.2.1.3 | Pb2+ | 30% inhibition at 5 mM | Tricholoma matsutake | |
| 3.2.1.3 | Zn2+ | 27% inhibition at 5 mM | Tricholoma matsutake | |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 3.2.1.3 | extracellular | - |
Tricholoma matsutake | - |
- |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.2.1.3 | Ca2+ | 29% activation at 5 mM | Tricholoma matsutake | |
| 3.2.1.3 | DTT | 25% activation at 5 mM | Tricholoma matsutake | |
| 3.2.1.3 | Mn2+ | slight activation at 5 mM | Tricholoma matsutake | |
| 3.2.1.3 | additional information | Ba2+, K+, Al3+, Mg2+, Fe3+, Na+, Ni+, Li+, Co2+, and EDTA have poor effects on activity | Tricholoma matsutake |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.3 | 63900 | - |
gel filtration | Tricholoma matsutake |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.2.1.3 | starch + H2O | Tricholoma matsutake | soluble starch | ? | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.2.1.3 | Tricholoma matsutake | H1AAU7 | - |
- |
| 3.2.1.3 | Tricholoma matsutake NBRC 30605 | H1AAU7 | - |
- |
Posttranslational Modification
| EC Number | Posttranslational Modification | Comment | Organism |
|---|---|---|---|
| 3.2.1.3 | glycoprotein | - |
Tricholoma matsutake |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.2.1.3 | native extracellular enzyme 33.2fold by ammonium sulfate fractionation, anion exchange chromatography, hydrophobic interaction chromatography, ultrafiltration, and gel filtration | Tricholoma matsutake |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 3.2.1.3 | mycelium | high glucoamylase activity is induced upon culture of the fungus in a medium containing amylose compared with other saccharides such as glucose, rice starch, wheat starch, corn starch, and potato starch | Tricholoma matsutake | - |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.3 | 13.2 | - |
purified enzyme, pH 5.0, 60°C, with soluble starch as substrate | Tricholoma matsutake |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.2.1.3 | amylopectin + H2O | - |
Tricholoma matsutake | ? | - |
? | |
| 3.2.1.3 | amylose A + H2O | - |
Tricholoma matsutake | ? | - |
? | |
| 3.2.1.3 | amylose B + H2O | - |
Tricholoma matsutake | ? | - |
? | |
| 3.2.1.3 | glycogen + H2O | - |
Tricholoma matsutake | ? | - |
? | |
| 3.2.1.3 | isomaltose + H2O | low activity | Tricholoma matsutake | ? | - |
? | |
| 3.2.1.3 | maltopentaose + H2O | - |
Tricholoma matsutake | ? | - |
? | |
| 3.2.1.3 | maltopentaose + H2O | - |
Tricholoma matsutake NBRC 30605 | ? | - |
? | |
| 3.2.1.3 | maltose + H2O | low activity | Tricholoma matsutake | ? | - |
? | |
| 3.2.1.3 | maltotetraose + H2O | - |
Tricholoma matsutake | ? | - |
? | |
| 3.2.1.3 | maltotetraose + H2O | - |
Tricholoma matsutake NBRC 30605 | ? | - |
? | |
| 3.2.1.3 | maltotriose + H2O | - |
Tricholoma matsutake | ? | - |
? | |
| 3.2.1.3 | maltotriose + H2O | - |
Tricholoma matsutake NBRC 30605 | ? | - |
? | |
| 3.2.1.3 | additional information | the enzyme degrades alpha-1,4- and alpha-1,6-glycosidic linkages in various polysaccharides and also malto-oligosaccharides, substrate specificity, overview. No activity with beta-cyclodextrin, alpha-cyclodextrin, trehalose, kojibiose, and nigerose | Tricholoma matsutake | ? | - |
? | |
| 3.2.1.3 | additional information | the enzyme degrades alpha-1,4- and alpha-1,6-glycosidic linkages in various polysaccharides and also malto-oligosaccharides, substrate specificity, overview. No activity with beta-cyclodextrin, alpha-cyclodextrin, trehalose, kojibiose, and nigerose | Tricholoma matsutake NBRC 30605 | ? | - |
? | |
| 3.2.1.3 | pullulan + H2O | - |
Tricholoma matsutake | ? | - |
? | |
| 3.2.1.3 | pullulan + H2O | - |
Tricholoma matsutake NBRC 30605 | ? | - |
? | |
| 3.2.1.3 | starch + H2O | soluble starch | Tricholoma matsutake | ? | - |
? | |
| 3.2.1.3 | starch + H2O | soluble starch, and low activity with raw corn starch | Tricholoma matsutake | ? | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 3.2.1.3 | monomer | 1 * 61500, SDS-PAGE | Tricholoma matsutake |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.2.1.3 | glucoamylase | - |
Tricholoma matsutake |
| 3.2.1.3 | TmGLA | - |
Tricholoma matsutake |
| 3.2.1.3 | TmGlu1 | - |
Tricholoma matsutake |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.3 | 60 | - |
recombinant enzyme | Tricholoma matsutake |
Temperature Range [°C]
| EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.3 | 25 | 75 | activity range, profile overview | Tricholoma matsutake |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.3 | 4 | 50 | purified enzyme, stable at | Tricholoma matsutake |
| 3.2.1.3 | 70 | - |
purified enzyme, inactivation | Tricholoma matsutake |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.3 | 5 | - |
recombinant enzyme | Tricholoma matsutake |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.3 | 2 | 6.5 | activity range, profile overview | Tricholoma matsutake |
pH Stability
| EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.3 | 2 | - |
purified enzyme, complete loss activity | Tricholoma matsutake |
| 3.2.1.3 | 3 | - |
purified enzyme, loss of 80% activity | Tricholoma matsutake |
| 3.2.1.3 | 4 | 6 | purified enzyme, stable at | Tricholoma matsutake |
| 3.2.1.3 | 7 | - |
purified enzyme, loss of 60% activity | Tricholoma matsutake |
| 3.2.1.3 | 8 | - |
purified enzyme, complete loss activity | Tricholoma matsutake |
General Information
| EC Number | General Information | Comment | Organism |
|---|---|---|---|
| 3.2.1.3 | evolution | the enzyme belongs to the glycosyl hydrolase family 15, GH15 | Tricholoma matsutake |
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