EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.1.30.2 | additional information | - |
DNA | at the optimal Mg2+ amount, both the Km and the Kcat values are similar to both RNA and DNA substrates | Serratia marcescens | |
3.1.30.2 | additional information | - |
RNA | at the optimal Mg2+ amount, both the Km and the Kcat values are similar to both RNA and DNA substrates | Serratia marcescens |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
3.1.30.2 | Mg2+ | in the presence of 0.58 mM Mg2+, the digestive activity of the enzyme is approximately fourfold increased as compared with the activity in the absence of Mg2+ taken as 100%. Further fivefold increase in Mg2+ concentration causes near 1.5fold enhancement in the enzyme activity. Subsequent two- and fourfold increases in Mg2+ concentration have only a minor impact on the enzyme activity within the experimental error range. 6.0-11.6 mM of Mg2+ corresponding to 20-40 Mg2+ per 1 phosphate in RNA is optimal for the hydrolysis of RNA. Optimal Mg2+ amount is linked with the changing secondary structure of RNA substrates within A-helix. Addition of Mg2+ affects both the rates of products dissociations from the enzyme-substrate complexes and the enzyme associations with the substrates, that is supported by strong increase in the Kcat values and change in the Km values | Serratia marcescens |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.1.30.2 | Serratia marcescens | P13717 | - |
- |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.1.30.2 | DNA + H2O | in absence of Mg2+ the enzyme shows a preference for DNA as compared to RNA | Serratia marcescens | ? | - |
? | |
3.1.30.2 | RNA + H2O | in absence of Mg2+ the enzyme shows a preference for DNA as compared to RNA | Serratia marcescens | ? | - |
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