EC Number | Cloned (Comment) | Organism |
---|---|---|
3.1.4.3 | expressed in Escherichia coli BL21-AI cells | Listeria monocytogenes |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
3.1.4.3 | C143S | the mutant shows increased activity compared to the wild type enzyme | Listeria monocytogenes |
3.1.4.3 | C168S | the mutant shows decreased activity compared to the wild type enzyme | Listeria monocytogenes |
3.1.4.3 | D55N | the mutation causes a dramatic loss of activity | Listeria monocytogenes |
3.1.4.3 | F66Y | the mutation causes a dramatic loss of activity | Listeria monocytogenes |
3.1.4.3 | H56Y | the activity of the mutant is comparable to the wild type enzyme | Listeria monocytogenes |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.1.4.3 | 2.8 | - |
1,2-dihexanoyl-sn-glycero-3-phosphocholine | at pH 6.0 and 37°C | Listeria monocytogenes | |
3.1.4.3 | 8.5 | - |
1,2-diheptanoyl-sn-glycero-3-phosphocholine | at pH 6.0 and 37°C | Listeria monocytogenes | |
3.1.4.3 | 43.5 | - |
1,2-dibutyryl-sn-glycero-3-phosphocholine | at pH 6.0 and 37°C | Listeria monocytogenes |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
3.1.4.3 | Zn2+ | a zinc metalloenzyme, 0.05 mM used in assay conditions | Listeria monocytogenes |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.1.4.3 | phosphatidylcholine + H2O | Listeria monocytogenes | - |
1,2-diacyl-sn-glycerol + phosphorylcholine | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.1.4.3 | Listeria monocytogenes | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.1.4.3 | ammonium sulfate precipitation and chitin resin column chromatography | Listeria monocytogenes |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.1.4.3 | 1,2-dibutyryl-sn-glycero-3-phosphocholine + H2O | - |
Listeria monocytogenes | 1,2-dibutyryl-sn-glycerol + phosphocholine | - |
? | |
3.1.4.3 | 1,2-diheptanoyl-sn-glycero-3-phosphocholine + H2O | - |
Listeria monocytogenes | 1,2-diheptanoyl-sn-glycerol + phosphocholine | - |
? | |
3.1.4.3 | 1,2-dihexanoyl-sn-glycero-3-phosphocholine + H2O | - |
Listeria monocytogenes | 1,2-dihexanoyl-sn-glycerol + phosphocholine | - |
? | |
3.1.4.3 | 1,2-dioleoyl-sn-glycero-3-phospho-(1'-rac-glycerol) + H2O | about 25% activity compared to 1,2-dioleoyl-sn-glycero-3-phosphocholine | Listeria monocytogenes | 1,2-dioleoyl-sn-glycerol + glycerol 1-phosphate | - |
? | |
3.1.4.3 | 1,2-dioleoyl-sn-glycero-3-phospho-L-serine + H2O | 60% activity compared to 1,2-dioleoyl-sn-glycero-3-phosphocholine | Listeria monocytogenes | 1,2-dioleoyl-sn-glycerol + phospho-L-serine | - |
? | |
3.1.4.3 | 1,2-dioleoyl-sn-glycero-3-phosphocholine + H2O | 100% activity | Listeria monocytogenes | 1,2-dioleoyl-sn-glycerol + phosphocholine | - |
? | |
3.1.4.3 | 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine + H2O | about 70% activity compared to 1,2-dioleoyl-sn-glycero-3-phosphocholine | Listeria monocytogenes | 1,2-dioleoyl-sn-glycerol + phosphoethanolamine | - |
? | |
3.1.4.3 | additional information | no activity with phosphatidylinositol | Listeria monocytogenes | ? | - |
? | |
3.1.4.3 | phosphatidylcholine + H2O | - |
Listeria monocytogenes | 1,2-diacyl-sn-glycerol + phosphorylcholine | - |
? | |
3.1.4.3 | sphingomyelin + H2O | about 70% activity compared to 1,2-dioleoyl-sn-glycero-3-phosphocholine | Listeria monocytogenes | ceramide + phosphocholine | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
3.1.4.3 | ? | x * 33000, proenzyme, SDS-PAGE | Listeria monocytogenes |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.1.4.3 | broad-range phospholipase C | - |
Listeria monocytogenes |
3.1.4.3 | PLC | - |
Listeria monocytogenes |
3.1.4.3 | PlcB | - |
Listeria monocytogenes |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.1.4.3 | 8 | - |
1,2-dibutyryl-sn-glycero-3-phosphocholine | at pH 6.0 and 37°C | Listeria monocytogenes | |
3.1.4.3 | 8.5 | - |
1,2-diheptanoyl-sn-glycero-3-phosphocholine | at pH 6.0 and 37°C | Listeria monocytogenes | |
3.1.4.3 | 12.8 | - |
1,2-dihexanoyl-sn-glycero-3-phosphocholine | at pH 6.0 and 37°C | Listeria monocytogenes |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.1.4.3 | 5 | - |
- |
Listeria monocytogenes |
EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.1.4.3 | 0.18 | - |
1,2-dibutyryl-sn-glycero-3-phosphocholine | at pH 6.0 and 37°C | Listeria monocytogenes | |
3.1.4.3 | 4.6 | - |
1,2-dihexanoyl-sn-glycero-3-phosphocholine | at pH 6.0 and 37°C | Listeria monocytogenes | |
3.1.4.3 | 42 | - |
1,2-diheptanoyl-sn-glycero-3-phosphocholine | at pH 6.0 and 37°C | Listeria monocytogenes |