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Literature summary extracted from

  • Huang, Q.; Gershenson, A.; Roberts, M.F.
    Recombinant broad-range phospholipase C from Listeria monocytogenes exhibits optimal activity at acidic pH (2016), Biochim. Biophys. Acta, 1864, 697-705 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.4.3 expressed in Escherichia coli BL21-AI cells Listeria monocytogenes

Protein Variants

EC Number Protein Variants Comment Organism
3.1.4.3 C143S the mutant shows increased activity compared to the wild type enzyme Listeria monocytogenes
3.1.4.3 C168S the mutant shows decreased activity compared to the wild type enzyme Listeria monocytogenes
3.1.4.3 D55N the mutation causes a dramatic loss of activity Listeria monocytogenes
3.1.4.3 F66Y the mutation causes a dramatic loss of activity Listeria monocytogenes
3.1.4.3 H56Y the activity of the mutant is comparable to the wild type enzyme Listeria monocytogenes

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.4.3 2.8
-
1,2-dihexanoyl-sn-glycero-3-phosphocholine at pH 6.0 and 37°C Listeria monocytogenes
3.1.4.3 8.5
-
1,2-diheptanoyl-sn-glycero-3-phosphocholine at pH 6.0 and 37°C Listeria monocytogenes
3.1.4.3 43.5
-
1,2-dibutyryl-sn-glycero-3-phosphocholine at pH 6.0 and 37°C Listeria monocytogenes

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.4.3 Zn2+ a zinc metalloenzyme, 0.05 mM used in assay conditions Listeria monocytogenes

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.4.3 phosphatidylcholine + H2O Listeria monocytogenes
-
1,2-diacyl-sn-glycerol + phosphorylcholine
-
?

Organism

EC Number Organism UniProt Comment Textmining
3.1.4.3 Listeria monocytogenes
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.4.3 ammonium sulfate precipitation and chitin resin column chromatography Listeria monocytogenes

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.4.3 1,2-dibutyryl-sn-glycero-3-phosphocholine + H2O
-
Listeria monocytogenes 1,2-dibutyryl-sn-glycerol + phosphocholine
-
?
3.1.4.3 1,2-diheptanoyl-sn-glycero-3-phosphocholine + H2O
-
Listeria monocytogenes 1,2-diheptanoyl-sn-glycerol + phosphocholine
-
?
3.1.4.3 1,2-dihexanoyl-sn-glycero-3-phosphocholine + H2O
-
Listeria monocytogenes 1,2-dihexanoyl-sn-glycerol + phosphocholine
-
?
3.1.4.3 1,2-dioleoyl-sn-glycero-3-phospho-(1'-rac-glycerol) + H2O about 25% activity compared to 1,2-dioleoyl-sn-glycero-3-phosphocholine Listeria monocytogenes 1,2-dioleoyl-sn-glycerol + glycerol 1-phosphate
-
?
3.1.4.3 1,2-dioleoyl-sn-glycero-3-phospho-L-serine + H2O 60% activity compared to 1,2-dioleoyl-sn-glycero-3-phosphocholine Listeria monocytogenes 1,2-dioleoyl-sn-glycerol + phospho-L-serine
-
?
3.1.4.3 1,2-dioleoyl-sn-glycero-3-phosphocholine + H2O 100% activity Listeria monocytogenes 1,2-dioleoyl-sn-glycerol + phosphocholine
-
?
3.1.4.3 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine + H2O about 70% activity compared to 1,2-dioleoyl-sn-glycero-3-phosphocholine Listeria monocytogenes 1,2-dioleoyl-sn-glycerol + phosphoethanolamine
-
?
3.1.4.3 additional information no activity with phosphatidylinositol Listeria monocytogenes ?
-
?
3.1.4.3 phosphatidylcholine + H2O
-
Listeria monocytogenes 1,2-diacyl-sn-glycerol + phosphorylcholine
-
?
3.1.4.3 sphingomyelin + H2O about 70% activity compared to 1,2-dioleoyl-sn-glycero-3-phosphocholine Listeria monocytogenes ceramide + phosphocholine
-
?

Subunits

EC Number Subunits Comment Organism
3.1.4.3 ? x * 33000, proenzyme, SDS-PAGE Listeria monocytogenes

Synonyms

EC Number Synonyms Comment Organism
3.1.4.3 broad-range phospholipase C
-
Listeria monocytogenes
3.1.4.3 PLC
-
Listeria monocytogenes
3.1.4.3 PlcB
-
Listeria monocytogenes

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.1.4.3 8
-
1,2-dibutyryl-sn-glycero-3-phosphocholine at pH 6.0 and 37°C Listeria monocytogenes
3.1.4.3 8.5
-
1,2-diheptanoyl-sn-glycero-3-phosphocholine at pH 6.0 and 37°C Listeria monocytogenes
3.1.4.3 12.8
-
1,2-dihexanoyl-sn-glycero-3-phosphocholine at pH 6.0 and 37°C Listeria monocytogenes

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.4.3 5
-
-
Listeria monocytogenes

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.1.4.3 0.18
-
1,2-dibutyryl-sn-glycero-3-phosphocholine at pH 6.0 and 37°C Listeria monocytogenes
3.1.4.3 4.6
-
1,2-dihexanoyl-sn-glycero-3-phosphocholine at pH 6.0 and 37°C Listeria monocytogenes
3.1.4.3 42
-
1,2-diheptanoyl-sn-glycero-3-phosphocholine at pH 6.0 and 37°C Listeria monocytogenes