Literature summary extracted from

Jun, S.Y.; Fushinobu, S.; Nojiri, H.; Omori, T.; Shoun, H.; Wakagi, T.
Improving the catalytic efficiency of a meta-cleavage product hydrolase (CumD) from Pseudomonas fluorescens IP01 (2006), Biochim. Biophys. Acta, 1764, 1159-1166 .

Crystallization (Commentary)

EC Number	Crystallization (Comment)	Organism
3.7.1.9	mutant enzyme A129V, vapor diffusion method	Pseudomonas fluorescens

Protein Variants

EC Number	Protein Variants	Comment	Organism
3.7.1.9	A129V	the kcat/Km value of the single mutant is higher than that of the wild type enzyme. The mutant shows the highest kcat/Km value for 2-hydroxy-6-oxohepta-2,4-dienoate	Pseudomonas fluorescens
3.7.1.9	I199V	the kcat/Km value of the single mutant is higher than that of the wild type enzyme	Pseudomonas fluorescens
3.7.1.9	V227I	the kcat/Km value of the single mutant is higher than that of the wild type enzyme	Pseudomonas fluorescens

Natural Substrates/ Products (Substrates)

EC Number	Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
3.7.1.25	(2Z,4E)-2-hydroxy-6-oxohepta-2,4-dienoate + H2O	Pseudomonas putida	-	(2Z)-2-hydroxypenta-2,4-dienoate + acetate	-	?

Organism

EC Number	Organism	UniProt	Comment	Textmining
3.7.1.9	Pseudomonas fluorescens	P96965	-	-
3.7.1.9	Pseudomonas fluorescens IP01	P96965	-	-
3.7.1.25	Pseudomonas putida	-	-	-

Substrates and Products (Substrate)

EC Number	Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
3.7.1.9	2-hydroxy-6-oxo-7-methylocta-2,4-dienoate + H2O	-	Pseudomonas fluorescens	?	-	?
3.7.1.9	2-hydroxy-6-oxo-7-methylocta-2,4-dienoate + H2O	-	Pseudomonas fluorescens IP01	?	-	?
3.7.1.25	(2Z,4E)-2-hydroxy-6-oxohepta-2,4-dienoate + H2O	-	Pseudomonas putida	(2Z)-2-hydroxypenta-2,4-dienoate + acetate	-	?

Synonyms

EC Number	Synonyms	Comment	Organism
3.7.1.9	CumD	-	Pseudomonas fluorescens
3.7.1.9	meta-cleavage product hydrolase	-	Pseudomonas fluorescens
3.7.1.25	meta-cleavage product hydrolase	-	Pseudomonas putida
3.7.1.25	TodF	-	Pseudomonas putida

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Release 2023.1 (January 2023)