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Literature summary extracted from

  • Taylor, Z.; Brown, H.; Holden, H.; Raushel, F.
    Biosynthesis of nucleoside diphosphoramidates in Campylobacter jejuni (2017), Biochemistry, 56, 6079-6082 .
    View publication on PubMedView publication on EuropePMC

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.7.103 0.12
-
 N5-phospho-L-glutamine pH 8.0, 25°C Campylobacter jejuni subsp. jejuni serotype O:2
2.7.7.103 0.17
-
 CTP pH 8.0, 25°C Campylobacter jejuni subsp. jejuni serotype O:2
3.5.1.129 0.028
-
 N5-(cytidine 5'-diphosphoramidyl)-L-glutamine pH 8.0, 25°C Campylobacter jejuni subsp. jejuni serotype O:2

Organism

EC Number Organism UniProt Comment Textmining
2.7.7.103 Campylobacter jejuni subsp. jejuni serotype O:2 Q0P8J8
-
-
2.7.7.103 Campylobacter jejuni subsp. jejuni serotype O:2 ATCC 700819 Q0P8J8
-
-
3.5.1.129 Campylobacter jejuni subsp. jejuni serotype O:2 Q0P8J7
-
-
3.5.1.129 Campylobacter jejuni subsp. jejuni serotype O:2 ATCC 700819 Q0P8J7
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.7.103 CTP + N5-phospho-L-glutamine
-
 Campylobacter jejuni subsp. jejuni serotype O:2 diphosphate + N5-(cytidine 5'-diphosphoramidyl)-L-glutamine
-
 ?
2.7.7.103 CTP + N5-phospho-L-glutamine
-
 Campylobacter jejuni subsp. jejuni serotype O:2 ATCC 700819 diphosphate + N5-(cytidine 5'-diphosphoramidyl)-L-glutamine
-
 ?
3.5.1.129 additional information L-glutamine phosphate is a very poor substrate Campylobacter jejuni subsp. jejuni serotype O:2 ?
-
 ?
3.5.1.129 additional information L-glutamine phosphate is a very poor substrate Campylobacter jejuni subsp. jejuni serotype O:2 ATCC 700819 ?
-
 ?
3.5.1.129 N5-(cytidine 5'-diphosphoramidyl)-L-glutamine + H2O
-
 Campylobacter jejuni subsp. jejuni serotype O:2 cytidine 5'-diphosphoramidate + L-glutamate
-
 ?
3.5.1.129 N5-(cytidine 5'-diphosphoramidyl)-L-glutamine + H2O
-
 Campylobacter jejuni subsp. jejuni serotype O:2 ATCC 700819 cytidine 5'-diphosphoramidate + L-glutamate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.7.7.103 Cj1416
-
 Campylobacter jejuni subsp. jejuni serotype O:2
3.5.1.129 Cj1417c
-
 Campylobacter jejuni subsp. jejuni serotype O:2
3.5.1.129 gamma-glutamyl-CDP-amidate hydrolase
-
 Campylobacter jejuni subsp. jejuni serotype O:2

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.7.7.103 0.095
-
 CTP pH 8.0, 25°C Campylobacter jejuni subsp. jejuni serotype O:2
2.7.7.103 0.095
-
 N5-phospho-L-glutamine pH 8.0, 25°C Campylobacter jejuni subsp. jejuni serotype O:2
3.5.1.129 0.55
-
 N5-(cytidine 5'-diphosphoramidyl)-L-glutamine pH 8.0, 25°C Campylobacter jejuni subsp. jejuni serotype O:2

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.7.103 8
-
 assay at Campylobacter jejuni subsp. jejuni serotype O:2
3.5.1.129 8
-
 assay at Campylobacter jejuni subsp. jejuni serotype O:2

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.7.7.103 5.7
-
 CTP pH 8.0, 25°C Campylobacter jejuni subsp. jejuni serotype O:2
2.7.7.103 8
-
 N5-phospho-L-glutamine pH 8.0, 25°C Campylobacter jejuni subsp. jejuni serotype O:2
3.5.1.129 20
-
 N5-(cytidine 5'-diphosphoramidyl)-L-glutamine pH 8.0, 25°C Campylobacter jejuni subsp. jejuni serotype O:2