EC Number | Cloned (Comment) | Organism |
---|---|---|
2.3.1.286 | expressed in Escherichia coli Codon-plus RIPL cells | Plasmodium falciparum |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
2.3.1.286 | alpha-1-O-methyl-adenosine diphosphoribose | - |
Plasmodium falciparum | |
2.3.1.286 | nicotinamide | - |
Plasmodium falciparum |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.3.1.286 | 0.085 | - |
[peptide p300]-N6-acetyl-L-lysine | at pH 8.0 and 37°C | Plasmodium falciparum | |
2.3.1.286 | 0.176 | - |
[histone H4]-N6-acetyl-L-lysine | at pH 8.0 and 37°C | Plasmodium falciparum | |
2.3.1.286 | 0.372 | - |
[histone H3]-N6-acetyl-L-lysine | at pH 8.0 and 37°C | Plasmodium falciparum |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.3.1.286 | Plasmodium falciparum | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.3.1.286 | Ni-column affinity chromatography | Plasmodium falciparum |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.3.1.286 | [histone H3]-N6-acetyl-L-lysine + NAD+ + H2O | histone H3 with the sequence ARTKQTAR(K-Ac)STGG(K-Ac)APRKQLAS. The enzyme deacetylates K9 and K16 of diacetylated histone H3 | Plasmodium falciparum | [histone H3]-L-lysine + 2''-O-acetyl-ADP-D-ribose + nicotinamide | - |
? | |
2.3.1.286 | [histone H4]-N6-acetyl-L-lysine + NAD+ + H2O | histone H3 with the sequence SGRG(K-Ac)GG(K-Ac)GLG(K-Ac)GGA(K-Ac)RHR. The enzyme predominantly deacetylates at K16 of tetra-acetylated histone H4 | Plasmodium falciparum | [histone H4]-L-lysine + 2''-O-acetyl-ADP-D-ribose + nicotinamide | - |
? | |
2.3.1.286 | [peptide p300]-N6-acetyl-L-lysine + NAD+ + H2O | peptide p300 with the sequence ERSTEL(K-Ac)TEI(K-Ac)EEEDQPSTS. The enzyme predominantly deacetylates at K1040 of full length peptide p300 | Plasmodium falciparum | [histone H4]-L-lysine + 2''-O-acetyl-ADP-D-ribose + nicotinamide | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.3.1.286 | Sir2 | - |
Plasmodium falciparum |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.3.1.286 | 0.00025 | - |
[histone H3]-N6-acetyl-L-lysine | at pH 8.0 and 37°C | Plasmodium falciparum | |
2.3.1.286 | 0.00035 | - |
[histone H4]-N6-acetyl-L-lysine | at pH 8.0 and 37°C | Plasmodium falciparum | |
2.3.1.286 | 0.00092 | - |
[peptide p300]-N6-acetyl-L-lysine | at pH 8.0 and 37°C | Plasmodium falciparum |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
2.3.1.286 | NAD+ | dependent on | Plasmodium falciparum |
EC Number | Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.3.1.286 | 0.035 | - |
nicotinamide | with [histone H3]-N6-acetyl-L-lysine as substrate, at pH 8.0 and 37°C | Plasmodium falciparum | |
2.3.1.286 | 0.091 | - |
nicotinamide | with [peptide p300]-N6-acetyl-L-lysine as substrate, at pH 8.0 and 37°C | Plasmodium falciparum |