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Literature summary extracted from

  • Newman, L.M.; Wackett, L.P.
    Purification and characterization of toluene 2-monooxygenase from Burkholderia cepacia G4 (1995), Biochemistry, 34, 14066-14076 .
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.14.13.243 0.0008
-
 2-methylphenol pH 7.5, 23°C Burkholderia cepacia

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.14.13.243 Iron the 211 kDa alpha2beta2gamma2 component contains five to six iron atoms Burkholderia cepacia
1.14.13.243 Iron the 40 kDa subunit contains a [2Fe2S] cluster, and the 211 kDa alpha2beta2gamma2 component contains five to six iron atoms Burkholderia cepacia

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.13.243 2-methylphenol + NADH + H+ + O2 Burkholderia cepacia
-
 3-methylcatechol + NAD+ + H2O
-
 ?
1.14.13.243 2-methylphenol + NADH + H+ + O2 Burkholderia cepacia G4
-
 3-methylcatechol + NAD+ + H2O
-
 ?
1.14.13.243 toluene + NADH + H+ + O2 Burkholderia cepacia
-
 2-methylphenol + NAD+ + H2O
-
 ?
1.14.13.243 toluene + NADH + H+ + O2 Burkholderia cepacia G4
-
 2-methylphenol + NAD+ + H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.14.13.243 Burkholderia cepacia
-
-
-
1.14.13.243 Burkholderia cepacia G4
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.13.243
-
 Burkholderia cepacia

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.13.243 2-methylphenol + NADH + H+ + O2
-
 Burkholderia cepacia 3-methylcatechol + NAD+ + H2O
-
 ?
1.14.13.243 2-methylphenol + NADH + H+ + O2
-
 Burkholderia cepacia G4 3-methylcatechol + NAD+ + H2O
-
 ?
1.14.13.243 toluene + NADH + H+ + O2
-
 Burkholderia cepacia 2-methylphenol + NAD+ + H2O
-
 ?
1.14.13.243 toluene + NADH + H+ + O2
-
 Burkholderia cepacia G4 2-methylphenol + NAD+ + H2O
-
 ?

Subunits

EC Number Subunits Comment Organism
1.14.13.243 More three-component enzyme system, consisting of a 40 kDa polypeptide, a 10.4 kDa polypeptide, and a 211 kDa alpha2beta2gamma2 component, SDS-PAGE Burkholderia cepacia

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.13.243 2Fe-2S-center
-
 Burkholderia cepacia
1.14.13.243 2Fe-2S-center the 40 kDa subunit contains a [2Fe2S] cluster Burkholderia cepacia
1.14.13.243 FAD the 40 kDa polypeptide subunit contains one FAD and a [2Fe-2S] cluster Burkholderia cepacia

pI Value

EC Number Organism Comment pI Value Maximum pI Value
1.14.13.243 Burkholderia cepacia 10.4 kDa polypeptide component, isoelectric focussing
-
 4.3
1.14.13.243 Burkholderia cepacia alpha2beta2gamma2 component, isoelectric focussing
-
 4.5
1.14.13.243 Burkholderia cepacia 40 kDa component, isoelectric focussing
-
 5.8

General Information

EC Number General Information Comment Organism
1.14.13.243 physiological function enzyme consists of a 40 kDa polypeptide containing one FAD and a [2Fe-2S] cluster, a 10.4 kDa polypeptide that contains no identifiable metals or organic cofactors, and a 211 kDa alpha2beta2gamma2 component containing five to six iron atoms. The 40 kDa flavo-iron-sulfur protein oxidizes NADH and transfers electrons to cytochrome c, dyes, and the alpha2beta2gamma2 component, which contains the site for toluene binding and hydroxylation Burkholderia cepacia
1.14.13.243 physiological function three-component enzyme system, consisting of a 40 kDa polypeptide containing one FAD and a [2Fe2S] cluster, a 10.4 kDa polypeptide that contains no identifiable metals or organic cofactors, and a 211 kDa alpha2beta2gamma2 component containing five to six iron atoms. The 40 kDa flavo-iron-sulfur protein oxidizes NADH and transfers electrons to cytochrome c, dyes, and the alpha2beta2gamma2 component. The 10.4 kDa component, added to the other two components and NADH, increases toluene oxidation rates 40 kDa polypeptide containing one FAD and a [2Fe2S] cluster, a 10.4 kDa polypeptide that contains no identifiable metals or organic cofactors, and a 211 kDa alpha2beta2gamma2 component containing five to six iron atoms10fold. The alpha2beta2gamma2 component contains the site for toluene binding and hydroxylation Burkholderia cepacia