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Literature summary extracted from

  • Gu, J.; Tong, H.; Ye, L.; Yu, H.
    The role of aromatic residue W20 in the activity and enantioselectivity control of esterase BioH toward aryl substrate (2015), Biochem. Eng. J., 101, 134-141 .
No PubMed abstract available

Protein Variants

EC Number Protein Variants Comment Organism
3.1.1.85 W20A mutation leads to complete loss of activity Escherichia coli K-12
3.1.1.85 W20F mutation has little effect on the activity and enantioselectivity Escherichia coli K-12
3.1.1.85 W20V mutation leads to complete loss of activity Escherichia coli K-12

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.85 Escherichia coli K-12
-
-
-
3.1.1.85 Escherichia coli K-12 substr. MG1655
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.85 methyl o-chloromandelate + H2o the pi-pi stacking interaction between the residue W20 and the substrate is crucial in the determination of enzymatic catalytic performance Escherichia coli K-12 ?
-
 ?
3.1.1.85 methyl o-chloromandelate + H2o the pi-pi stacking interaction between the residue W20 and the substrate is crucial in the determination of enzymatic catalytic performance Escherichia coli K-12 substr. MG1655 ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.1.1.85 BioH
-
 Escherichia coli K-12

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.1.85 30
-
 assay at Escherichia coli K-12

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.1.85 7.5
-
 assay at Escherichia coli K-12