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Literature summary extracted from
- Purification of thermo and acid tolerant extracellular phytase from a new soil isolate of Amycolatopsis vancoresmycina S-12 (2017), Biocatal. Agricult. Biotechnol., 11, 302-306 .
No PubMed abstract available
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.1.3.8 | Ca2+ | 40% inhibition at 10 mM | Amycolatopsis vancoresmycina |  |
| 3.1.3.8 | Cu2+ | 20% inhibition at 10 mM | Amycolatopsis vancoresmycina | |
| 3.1.3.8 | Fe2+ | 30% inhibition at 10 mM | Amycolatopsis vancoresmycina | |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 3.1.3.8 | extracellular | - |
Amycolatopsis vancoresmycina | - |
- |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.1.3.8 | Mg2+ | activates 17% at 10 mM | Amycolatopsis vancoresmycina | |
| 3.1.3.8 | additional information | no or poor effect by Mn2+ and Zn2+ at 10 mM | Amycolatopsis vancoresmycina |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.1.3.8 | 53000 | - |
native PAGE | Amycolatopsis vancoresmycina |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.1.3.8 | myo-inositol hexakisphosphate + H2O | Amycolatopsis vancoresmycina | - |
1D-myo-inositol 1,2,4,5,6-pentakisphosphate + phosphate | - |
? | |
| 3.1.3.8 | myo-inositol hexakisphosphate + H2O | Amycolatopsis vancoresmycina S-12 | - |
1D-myo-inositol 1,2,4,5,6-pentakisphosphate + phosphate | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.1.3.8 | Amycolatopsis vancoresmycina | - |
isolated from soil, India | - |
| 3.1.3.8 | Amycolatopsis vancoresmycina S-12 | - |
isolated from soil, India | - |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.1.3.8 | native extracellular enzyme from culture medium 5.51fold by ammonium sulfate fractionation, dialysis, and anion exchange chromatography, and gel filtration, to near homogeneity | Amycolatopsis vancoresmycina |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.1.3.8 | myo-inositol hexakisphosphate + H2O | - |
Amycolatopsis vancoresmycina | 1D-myo-inositol 1,2,4,5,6-pentakisphosphate + phosphate | - |
? | |
| 3.1.3.8 | myo-inositol hexakisphosphate + H2O | phytate | Amycolatopsis vancoresmycina | 1D-myo-inositol 1,2,4,5,6-pentakisphosphate + phosphate | - |
? | |
| 3.1.3.8 | myo-inositol hexakisphosphate + H2O | - |
Amycolatopsis vancoresmycina S-12 | 1D-myo-inositol 1,2,4,5,6-pentakisphosphate + phosphate | - |
? | |
| 3.1.3.8 | myo-inositol hexakisphosphate + H2O | phytate | Amycolatopsis vancoresmycina S-12 | 1D-myo-inositol 1,2,4,5,6-pentakisphosphate + phosphate | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 3.1.3.8 | monomer | 1 * 53000, SDS-PAGE and native PAGE | Amycolatopsis vancoresmycina |
| 3.1.3.8 | More | peptide mass fingerprinting, MALDI-TOF mass spectrometry | Amycolatopsis vancoresmycina |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.1.3.8 | 37 | - |
- |
Amycolatopsis vancoresmycina |
Temperature Range [°C]
| EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.1.3.8 | 20 | 90 | activity range, profile overview | Amycolatopsis vancoresmycina |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.1.3.8 | 60 | - |
purified native extracellular enzyme, completely stable up to | Amycolatopsis vancoresmycina |
| 3.1.3.8 | 70 | - |
purified native extracellular enzyme, loss of 50% activity | Amycolatopsis vancoresmycina |
| 3.1.3.8 | 90 | - |
purified native extracellular enzyme, loss of about 90% activity | Amycolatopsis vancoresmycina |
| 3.1.3.8 | 100 | - |
purified native extracellular enzyme, inactivation | Amycolatopsis vancoresmycina |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.1.3.8 | 5.5 | - |
- |
Amycolatopsis vancoresmycina |
pH Stability
| EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.1.3.8 | 8 | - |
purified native extracellular enzyme, most stable at | Amycolatopsis vancoresmycina |
pI Value
| EC Number | Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|---|
| 3.1.3.8 | Amycolatopsis vancoresmycina | isoelectric focussing | - |
5.4 |
General Information
| EC Number | General Information | Comment | Organism |
|---|---|---|---|
| 3.1.3.8 | physiological function | the enzyme promotes plant growth showing a significant effect, with an increase of 10% of root and 25% of shoot length, resulting in a 2.2fold increase in biomass | Amycolatopsis vancoresmycina |
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