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Literature summary extracted from
- Purification, characterization and gene cloning of isoeugenol-degrading enzyme from Pseudomonas putida IE27 (2007), Arch. Microbiol., 187, 511-517 .
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.13.11.88 | 8-hydroxyquinoline | 1 mM, 90% loss of activity | Pseudomonas putida |  |
| 1.13.11.88 | Ag+ | 1 mM, 73% loss of activity | Pseudomonas putida | |
| 1.13.11.88 | Cu2+ | 1 mM, 56% loss of activity | Pseudomonas putida | |
| 1.13.11.88 | Hg2+ | 1 mM, 87% loss of activity | Pseudomonas putida | |
| 1.13.11.88 | additional information | not inhibitory: NAD+, NADH, NADP+, NADPH, FMN and FAD | Pseudomonas putida | |
| 1.13.11.88 | p-chloromercuribenzoic acid | 1 mM, 44% loss of activity | Pseudomonas putida | |
| 1.13.11.88 | phenylhydrazine | 1 mM, 95% loss of activity | Pseudomonas putida | |
| 1.13.11.88 | R-cycloserine | 1 mM, 48% loss of activity | Pseudomonas putida | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.13.11.88 | 0.175 | - |
isoeugenol | pH 7.0, 30°C | Pseudomonas putida | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 1.13.11.88 | 69000 | - |
gel filtration | Pseudomonas putida |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.13.11.88 | isoeugenol + O2 | Pseudomonas putida | - |
vanillin + acetaldehyde | - |
? | |
| 1.13.11.88 | additional information | Pseudomonas putida | enzyme catalyzes the initial step of isoeugenol degradation, the oxidative cleavage of the side chain double-bond of isoeugenol, to form vanillin. Enzyme catalyzes the incorporation of an oxygen atom from either molecular oxygen or water into vanillin | ? | - |
? | |
| 1.13.11.88 | additional information | Pseudomonas putida | no substrates: eugenol, coniferyl alcohol, coniferyl aldehyde, ferulic acid, 3,4-dihydroxycinnamic acid, 3,4-dimethoxycinnamic acid, 4-methoxycinnamic acid, 4-hydroxycinnamic acid, 3-pyridinepropionic acid, alpha-methoxycinnamic acid, styrene, trans-beta-methylstyrene, trans-stilbene, trans-stilbene oxide, acrylic acid and trans-2-hexenoic acid | ? | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.13.11.88 | Pseudomonas putida | A5HV13 | - |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.13.11.88 | - |
Pseudomonas putida |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 1.13.11.88 | 5.53 | - |
pH 7.0, 30°C | Pseudomonas putida |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.13.11.88 | 2-methoxy-4-vinylphenol + O2 | about 1% of the activity with isoeugenol | Pseudomonas putida | vanillin + formaldehyde | - |
? | |
| 1.13.11.88 | isoeugenol + O2 | - |
Pseudomonas putida | vanillin + acetaldehyde | - |
? | |
| 1.13.11.88 | additional information | enzyme catalyzes the initial step of isoeugenol degradation, the oxidative cleavage of the side chain double-bond of isoeugenol, to form vanillin. Enzyme catalyzes the incorporation of an oxygen atom from either molecular oxygen or water into vanillin | Pseudomonas putida | ? | - |
? | |
| 1.13.11.88 | additional information | no substrates: eugenol, coniferyl alcohol, coniferyl aldehyde, ferulic acid, 3,4-dihydroxycinnamic acid, 3,4-dimethoxycinnamic acid, 4-methoxycinnamic acid, 4-hydroxycinnamic acid, 3-pyridinepropionic acid, alpha-methoxycinnamic acid, styrene, trans-beta-methylstyrene, trans-stilbene, trans-stilbene oxide, acrylic acid and trans-2-hexenoic acid | Pseudomonas putida | ? | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 1.13.11.88 | monomer | 1 * 55000, SDS-PAGE, 1 * 548080, calculated from sequence | Pseudomonas putida |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 1.13.11.88 | ISO | - |
Pseudomonas putida |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.13.11.88 | 30 | - |
- |
Pseudomonas putida |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.13.11.88 | 5.18 | - |
isoeugenol | pH 7.0, 30°C | Pseudomonas putida | |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.13.11.88 | 9 | - |
- |
Pseudomonas putida |
pH Stability
| EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.13.11.88 | 6 | 10.5 | 20°C, stable for 30 min | Pseudomonas putida |
kcat/KM [mM/s]
| EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.13.11.88 | 29.8 | - |
isoeugenol | pH 7.0, 30°C | Pseudomonas putida | |
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