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Literature summary extracted from

  • Thanassi, N.M.; Nakada, H.I.
    Enzymic degradation of fucoidan by enzymes from the hepatopancreas of abalone, Halotus species (1967), Arch. Biochem. Biophys., 118, 172-177 .
No PubMed abstract available

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.2.1.211 cysteine 0.01 M, 1.05fold activation Haliotis sp.
3.2.1.211 N-ethylmaleimide 0.01 M, 1.4fold activation Haliotis sp.
3.2.1.212 cysteine 0.01 M, 1.05fold activation Haliotis sp.
3.2.1.212 N-ethylmaleimide 0.01 M, 1.4fold activation Haliotis sp.

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.211 AgNO3 0.001 M, 58% loss of activity Haliotis sp.
3.2.1.211 cysteine 0.001 M, 7% loss of activity Haliotis sp.
3.2.1.211 EDTA 0.02 M, 22% loss of activity Haliotis sp.
3.2.1.211 HgCl2 0.001 M, 80% loss of activity Haliotis sp.
3.2.1.211 MnCl2 0.001, 24% loss of activity Haliotis sp.
3.2.1.212 AgNO3 0.001 M, 58% loss of activity Haliotis sp.
3.2.1.212 cysteine 0.001 M, 7% loss of activity Haliotis sp.
3.2.1.212 EDTA 0.02 M, 22% loss of activity Haliotis sp.
3.2.1.212 HgCl2 0.001 M, 80% loss of activity Haliotis sp.
3.2.1.212 MnCl2 0.001, 24% loss of activity Haliotis sp.

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.211 MgSO4 0.01 M, 1.2fold activation Haliotis sp.
3.2.1.212 MgSO4 0.01 M, 1.2fold activation Haliotis sp.

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.211 Haliotis sp.
-
-
-
3.2.1.212 Haliotis sp.
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.211 partial Haliotis sp.
3.2.1.212 partial Haliotis sp.

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.211 hepatopancreas
-
Haliotis sp.
-
3.2.1.212 hepatopancreas
-
Haliotis sp.
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.211 fucoidan + H2O fucoidan from Fucus gardneri. The fucoidanase hydrolyzes fucoidan without desulfation to form oligosaccharides ranging from 10 to 2 fucose units plus the monosaccharide, fucose. Fucose can not be detected until late in the hydrolysis period, and sulfate release is not detectable during the first 4 hours of fucoidanase action Haliotis sp. sulfated alpha-L-fucooligosaccharides
-
?
3.2.1.211 additional information no degradation of 4-nitrophenyl-L-fucoside or blood group substance Haliotis sp. ?
-
?
3.2.1.212 fucoidan + H2O fucoidan from Fucus gardneri. The fucoidanase hydrolyzes fucoidan without desulfation to form oligosaccharides ranging from 10 to 2 fucose units plus the monosaccharide, fucose. Fucose can not be detected until late in the hydrolysis period, and sulfate release is not detectable during the first 4 hours of fucoidanase action Haliotis sp. sulfated alpha-L-fucooligosaccharides
-
?
3.2.1.212 additional information no degradation of 4-nitrophenyl-L-fucoside or blood group substance Haliotis sp. ?
-
?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.211 38
-
-
Haliotis sp.
3.2.1.212 38
-
-
Haliotis sp.

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.2.1.211 25 50 25°C: about 30% of maximal activity, 50°C: about 80% of maximal activity Haliotis sp.
3.2.1.212 25 50 25°C: about 30% of maximal activity, 50°C: about 80% of maximal activity Haliotis sp.

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.211 50
-
5 min, 15% loss of activity Haliotis sp.
3.2.1.212 50
-
5 min, 15% loss of activity Haliotis sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.211 5.4
-
citrate buffer, phosphate buffer, or citrate phosphate Haliotis sp.
3.2.1.212 5.4
-
citrate buffer, phosphate buffer, or citrate phosphate Haliotis sp.

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.211 4.5 6.5 pH 4.5: about 75% of maximal activity, pH 6.5: about 40% of maximal activity Haliotis sp.
3.2.1.212 4.5 6.5 pH 4.5: about 75% of maximal activity, pH 6.5: about 40% of maximal activity Haliotis sp.

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.211 4
-
30 min at room temperature, 60% loss of activity Haliotis sp.
3.2.1.211 5 8.5 30 min at room temperature, stable Haliotis sp.
3.2.1.211 9
-
30 min at room temperature, 60% loss of activity Haliotis sp.
3.2.1.212 4
-
30 min at room temperature, 60% loss of activity Haliotis sp.
3.2.1.212 5 8.5 30 min at room temperature, stable Haliotis sp.
3.2.1.212 9
-
30 min at room temperature, 60% loss of activity Haliotis sp.