EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
3.2.1.211 | cysteine | 0.01 M, 1.05fold activation | Haliotis sp. | |
3.2.1.211 | N-ethylmaleimide | 0.01 M, 1.4fold activation | Haliotis sp. | |
3.2.1.212 | cysteine | 0.01 M, 1.05fold activation | Haliotis sp. | |
3.2.1.212 | N-ethylmaleimide | 0.01 M, 1.4fold activation | Haliotis sp. |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
3.2.1.211 | AgNO3 | 0.001 M, 58% loss of activity | Haliotis sp. | |
3.2.1.211 | cysteine | 0.001 M, 7% loss of activity | Haliotis sp. | |
3.2.1.211 | EDTA | 0.02 M, 22% loss of activity | Haliotis sp. | |
3.2.1.211 | HgCl2 | 0.001 M, 80% loss of activity | Haliotis sp. | |
3.2.1.211 | MnCl2 | 0.001, 24% loss of activity | Haliotis sp. | |
3.2.1.212 | AgNO3 | 0.001 M, 58% loss of activity | Haliotis sp. | |
3.2.1.212 | cysteine | 0.001 M, 7% loss of activity | Haliotis sp. | |
3.2.1.212 | EDTA | 0.02 M, 22% loss of activity | Haliotis sp. | |
3.2.1.212 | HgCl2 | 0.001 M, 80% loss of activity | Haliotis sp. | |
3.2.1.212 | MnCl2 | 0.001, 24% loss of activity | Haliotis sp. |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
3.2.1.211 | MgSO4 | 0.01 M, 1.2fold activation | Haliotis sp. | |
3.2.1.212 | MgSO4 | 0.01 M, 1.2fold activation | Haliotis sp. |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.2.1.211 | Haliotis sp. | - |
- |
- |
3.2.1.212 | Haliotis sp. | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.2.1.211 | partial | Haliotis sp. |
3.2.1.212 | partial | Haliotis sp. |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
3.2.1.211 | hepatopancreas | - |
Haliotis sp. | - |
3.2.1.212 | hepatopancreas | - |
Haliotis sp. | - |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.2.1.211 | fucoidan + H2O | fucoidan from Fucus gardneri. The fucoidanase hydrolyzes fucoidan without desulfation to form oligosaccharides ranging from 10 to 2 fucose units plus the monosaccharide, fucose. Fucose can not be detected until late in the hydrolysis period, and sulfate release is not detectable during the first 4 hours of fucoidanase action | Haliotis sp. | sulfated alpha-L-fucooligosaccharides | - |
? | |
3.2.1.211 | additional information | no degradation of 4-nitrophenyl-L-fucoside or blood group substance | Haliotis sp. | ? | - |
? | |
3.2.1.212 | fucoidan + H2O | fucoidan from Fucus gardneri. The fucoidanase hydrolyzes fucoidan without desulfation to form oligosaccharides ranging from 10 to 2 fucose units plus the monosaccharide, fucose. Fucose can not be detected until late in the hydrolysis period, and sulfate release is not detectable during the first 4 hours of fucoidanase action | Haliotis sp. | sulfated alpha-L-fucooligosaccharides | - |
? | |
3.2.1.212 | additional information | no degradation of 4-nitrophenyl-L-fucoside or blood group substance | Haliotis sp. | ? | - |
? |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.2.1.211 | 38 | - |
- |
Haliotis sp. |
3.2.1.212 | 38 | - |
- |
Haliotis sp. |
EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.2.1.211 | 25 | 50 | 25°C: about 30% of maximal activity, 50°C: about 80% of maximal activity | Haliotis sp. |
3.2.1.212 | 25 | 50 | 25°C: about 30% of maximal activity, 50°C: about 80% of maximal activity | Haliotis sp. |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.2.1.211 | 50 | - |
5 min, 15% loss of activity | Haliotis sp. |
3.2.1.212 | 50 | - |
5 min, 15% loss of activity | Haliotis sp. |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.2.1.211 | 5.4 | - |
citrate buffer, phosphate buffer, or citrate phosphate | Haliotis sp. |
3.2.1.212 | 5.4 | - |
citrate buffer, phosphate buffer, or citrate phosphate | Haliotis sp. |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
3.2.1.211 | 4.5 | 6.5 | pH 4.5: about 75% of maximal activity, pH 6.5: about 40% of maximal activity | Haliotis sp. |
3.2.1.212 | 4.5 | 6.5 | pH 4.5: about 75% of maximal activity, pH 6.5: about 40% of maximal activity | Haliotis sp. |
EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|---|
3.2.1.211 | 4 | - |
30 min at room temperature, 60% loss of activity | Haliotis sp. |
3.2.1.211 | 5 | 8.5 | 30 min at room temperature, stable | Haliotis sp. |
3.2.1.211 | 9 | - |
30 min at room temperature, 60% loss of activity | Haliotis sp. |
3.2.1.212 | 4 | - |
30 min at room temperature, 60% loss of activity | Haliotis sp. |
3.2.1.212 | 5 | 8.5 | 30 min at room temperature, stable | Haliotis sp. |
3.2.1.212 | 9 | - |
30 min at room temperature, 60% loss of activity | Haliotis sp. |