EC Number | Cloned (Comment) | Organism |
---|---|---|
3.2.1.1 | expression in Escherichia coli | Corallococcus sp. EGB |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
3.2.1.1 | acarbose | - |
Corallococcus sp. EGB |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.2.1.1 | additional information | - |
amylopectin | Km-value: 10.3 mg/ml, pH 7.0, 50°C | Corallococcus sp. EGB | |
3.2.1.1 | additional information | - |
starch | Km-value: 2.9 mg/ml, pH 7.0, 50°C | Corallococcus sp. EGB |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
3.2.1.1 | 59946 | - |
calculated from sequence | Corallococcus sp. EGB |
3.2.1.1 | 60000 | - |
SDS-PAGE | Corallococcus sp. EGB |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.2.1.1 | Corallococcus sp. EGB | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.2.1.1 | - |
Corallococcus sp. EGB |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
3.2.1.1 | 121.3 | - |
substrate: amylopectin, pH 7.0, 50°C | Corallococcus sp. EGB |
3.2.1.1 | 980 | - |
substrate: starch, pH 7.0, 50°C | Corallococcus sp. EGB |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.2.1.1 | amylopectin + H2O | - |
Corallococcus sp. EGB | maltose + ? | - |
? | |
3.2.1.1 | gamma-cyclodextrin + H2O | - |
Corallococcus sp. EGB | maltose + ? | - |
? | |
3.2.1.1 | maltoheptaose + H2O | - |
Corallococcus sp. EGB | maltose + ? | - |
? | |
3.2.1.1 | maltohexaose + H2O | - |
Corallococcus sp. EGB | maltose + ? | - |
? | |
3.2.1.1 | maltotetraose + H2O | - |
Corallococcus sp. EGB | maltose + ? | - |
? | |
3.2.1.1 | maltotriose + H2O | no activity against isomaltotriose (alpha-glucosyl-(1->6)-alpha-glucosyl-(1->6)-glucose) | Corallococcus sp. EGB | maltose + ? | - |
? | |
3.2.1.1 | additional information | the enzyme hydrolyses alpha-1,4-glycosidic bonds and not alpha-1,6-linkages. It has no activity toward maltose, alpha-cyclodextrin, beta-cyclodextrin, panose, acarbose and dextran | Corallococcus sp. EGB | ? | - |
? | |
3.2.1.1 | starch + H2O | action mode is endolytic, hydrolysis products have an alpha-anomeric configuration. Degradation of 1% (w/v) starch results in the formation of oligosaccharides in the maltose to maltopentaose range at early time points, followed by an accumulation of maltose, maltotetraose, and small amounts of glucose after 1 h, and its final end products consisted of a high level of 58% (w/w) maltose without concomitant production of glucose | Corallococcus sp. EGB | alpha-maltose + ? | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.2.1.1 | ComA | - |
Corallococcus sp. EGB |
3.2.1.1 | maltogenic amylase | - |
Corallococcus sp. EGB |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.2.1.1 | 50 | - |
- |
Corallococcus sp. EGB |
EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.2.1.1 | 25 | 60 | 25°C: about 45% of maximal activity, 60°C: about 45% of maximal activity | Corallococcus sp. EGB |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.2.1.1 | 40 | - |
almost all of the enzyme activity remains after the enzyme is incubated for 60 min at temperatures of below 40°C | Corallococcus sp. EGB |
3.2.1.1 | 50 | - |
30 min, not stable above 50°C | Corallococcus sp. EGB |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.2.1.1 | 2274.8 | - |
amylopectin | pH 7.0, 50°C | Corallococcus sp. EGB | |
3.2.1.1 | 3021.2 | - |
starch | pH 7.0, 50°C | Corallococcus sp. EGB |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.2.1.1 | 7 | - |
- |
Corallococcus sp. EGB |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
3.2.1.1 | 6 | 9 | pH 6.0: about 80% of maximal activity, pH 9.0: about 45% of maximal activity | Corallococcus sp. EGB |
EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|---|
3.2.1.1 | 5 | 9 | 4°C, 24 h, enzyme retains more than 50% activity | Corallococcus sp. EGB |
EC Number | Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|---|
3.2.1.1 | Corallococcus sp. EGB | calculated from sequence | - |
6.08 |
EC Number | General Information | Comment | Organism |
---|---|---|---|
3.2.1.1 | additional information | the enzyme may be involved in the accumulation of maltose in Corallococcus media | Corallococcus sp. EGB |
EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.2.1.1 | 220 | - |
amylopectin | pH 7.0, 50°C | Corallococcus sp. EGB | |
3.2.1.1 | 1019.6 | - |
starch | pH 7.0, 50°C | Corallococcus sp. EGB |