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Literature summary extracted from

  • Yan, X.; Yang, R.; Zhao, R.X.; Han, J.T.; Jia, W.J.; Li, D.Y.; Wang, Y.; Zhang, N.; Wu, Y.; Zhang, L.Q.; He, Y.X.
    Transcriptional regulator PhlH modulates 2,4-diacetylphloroglucinol biosynthesis in response to the biosynthetic intermediate and end product (2017), Appl. Environ. Microbiol., 83, e01419-17 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.7.1.24 expressed in Escherichia coli BL21(DE3) cells Pseudomonas fluorescens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.7.1.24 2,4-diacetylphloroglucinol + H2O Pseudomonas fluorescens
-
2-acetylphloroglucinol + acetate
-
?
3.7.1.24 2,4-diacetylphloroglucinol + H2O Pseudomonas fluorescens degradation of 2,4-diacetylphloroglucinol catalyzed by PhlG plays an insignificant role in 2,4-diacetylphloroglucinol tolerance but contributes to bacterial growth advantages under carbon/nitrogen starvation conditions 2-acetylphloroglucinol + acetate
-
?
3.7.1.24 2,4-diacetylphloroglucinol + H2O Pseudomonas fluorescens 2P24
-
2-acetylphloroglucinol + acetate
-
?
3.7.1.24 2,4-diacetylphloroglucinol + H2O Pseudomonas fluorescens 2P24 degradation of 2,4-diacetylphloroglucinol catalyzed by PhlG plays an insignificant role in 2,4-diacetylphloroglucinol tolerance but contributes to bacterial growth advantages under carbon/nitrogen starvation conditions 2-acetylphloroglucinol + acetate
-
?

Organism

EC Number Organism UniProt Comment Textmining
3.7.1.24 Pseudomonas fluorescens
-
-
-
3.7.1.24 Pseudomonas fluorescens Q4JIX4
-
-
3.7.1.24 Pseudomonas fluorescens 2P24
-
-
-
3.7.1.24 Pseudomonas fluorescens 2P24 Q4JIX4
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.7.1.24
-
Pseudomonas fluorescens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.7.1.24 2,4-diacetylphloroglucinol + H2O
-
Pseudomonas fluorescens 2-acetylphloroglucinol + acetate
-
?
3.7.1.24 2,4-diacetylphloroglucinol + H2O degradation of 2,4-diacetylphloroglucinol catalyzed by PhlG plays an insignificant role in 2,4-diacetylphloroglucinol tolerance but contributes to bacterial growth advantages under carbon/nitrogen starvation conditions Pseudomonas fluorescens 2-acetylphloroglucinol + acetate
-
?
3.7.1.24 2,4-diacetylphloroglucinol + H2O
-
Pseudomonas fluorescens 2P24 2-acetylphloroglucinol + acetate
-
?
3.7.1.24 2,4-diacetylphloroglucinol + H2O degradation of 2,4-diacetylphloroglucinol catalyzed by PhlG plays an insignificant role in 2,4-diacetylphloroglucinol tolerance but contributes to bacterial growth advantages under carbon/nitrogen starvation conditions Pseudomonas fluorescens 2P24 2-acetylphloroglucinol + acetate
-
?

Synonyms

EC Number Synonyms Comment Organism
3.7.1.24 2,4-DAPG hydrolase
-
Pseudomonas fluorescens
3.7.1.24 Phlg
-
Pseudomonas fluorescens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.7.1.24 7.1
-
assay at Pseudomonas fluorescens

Expression

EC Number Organism Comment Expression
3.7.1.24 Pseudomonas fluorescens the transcriptional regulator PhlH represses the expression of the enzyme down
3.7.1.24 Pseudomonas fluorescens the pathway-specific regulator PhlH represses the phlG gene expression in vivo.Repressed expression of phlG is dependent on the interaction between PhlH and the palindromic sequence within the phlG promoter region down
3.7.1.24 Pseudomonas fluorescens at 8 h after inoculation, 0.1 mM 2,4-diacetylphloroglucinol has an about 8fold induction of enzyme expression, whereas 0.1 mM monoacetylphloroglucinol has about 2fold induction of enzyme expression up

General Information

EC Number General Information Comment Organism
3.7.1.24 physiological function the enzyme contributes to bacterial growth advantages under carbon/nitrogen starvation conditions Pseudomonas fluorescens
3.7.1.24 physiological function the pathway-specific regulator PhlH modulates 2,4-diacetylphloroglucinol levels by controlling the expression of 2,4-diacetylphloroglucinol hydrolase PhlG in response to 2,4-diacetylphloroglucinol and monoacetylphloroglucinol. Since 2,4-diacetylphloroglucinol biosynthesis imposes a metabolic burden on biocontrol pseudomonads, it is expected that the fine regulation of phlG by PhlH offers a way to dynamically modulate the metabolic loads attributed to 2,4-diacetylphloroglucinol production Pseudomonas fluorescens