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Literature summary extracted from

  • Feingersch, R.; Shainsky, J.; Wood, T.K.; Fishman, A.
    Protein engineering of toluene monooxygenases for synthesis of chiral sulfoxides (2008), Appl. Environ. Microbiol., 74, 1555-1566 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.13.243 expression in Escherichia coli Burkholderia cepacia

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.14.13.243 homology modeling of subunit structure Burkholderia cepacia

Protein Variants

EC Number Protein Variants Comment Organism
1.14.13.243 V106M mutation of the alpha-hydroxylase subunit TomA3, improves both rate and enantioselectivity. Mutant oxidizes methyl phenyl sulfide to the corresponding sulfoxide at a rate of 3.0 nmol/min/mg protein compared with 1.6 for the wild-type enzyme, and the enantiomeric excess (pro-S) increases from 51% for the wild type to 88% for the mutant Burkholderia cepacia

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.14.13.243 0.068
-
 methyl p-tolyl sulfide wild-type, whole cell assay, recombinant protein, pH not specified in the publication, temperature not specified in the publication Burkholderia cepacia
1.14.13.243 0.129
-
 thioanisole wild-type, whole cell assay, recombinant protein, pH not specified in the publication, temperature not specified in the publication Burkholderia cepacia

Organism

EC Number Organism UniProt Comment Textmining
1.14.13.243 Burkholderia cepacia Q9ANX4 and Q9ANX0 and Q9ANX3 and Q8VSV8 and Q9ANX1 and Q8VSV9 Q9ANX4 i.e. subunit tomA1, Q9ANX0 i.e. subunit tomA5, Q9ANX3 i.e. subunit TomA2, Q8VSV8 i.e. subunit TomA3, Q9ANX1 i.e. subunit TomA4, Q8VSV9 i.e. subunit TomA0
-
1.14.13.243 Burkholderia cepacia G4 Q9ANX4 and Q9ANX0 and Q9ANX3 and Q8VSV8 and Q9ANX1 and Q8VSV9 Q9ANX4 i.e. subunit tomA1, Q9ANX0 i.e. subunit tomA5, Q9ANX3 i.e. subunit TomA2, Q8VSV8 i.e. subunit TomA3, Q9ANX1 i.e. subunit TomA4, Q8VSV9 i.e. subunit TomA0
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.13.243 methyl p-tolyl sulfide + NADH + H+ + O2
-
 Burkholderia cepacia methyl p-tolyl sulfoxide + NAD+ + H2O
-
 ?
1.14.13.243 methyl p-tolyl sulfide + NADH + H+ + O2
-
 Burkholderia cepacia G4 methyl p-tolyl sulfoxide + NAD+ + H2O
-
 ?
1.14.13.243 thioanisole + NADH + H+ + O2
-
 Burkholderia cepacia methyl phenyl sulfoxide + NAD+ + H2O
-
 ?
1.14.13.243 thioanisole + NADH + H+ + O2
-
 Burkholderia cepacia G4 methyl phenyl sulfoxide + NAD+ + H2O
-
 ?