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Literature summary extracted from

  • Rui, L.; Kwon, Y.M.; Fishman, A.; Reardon, K.F.; Wood, T.K.
    Saturation mutagenesis of toluene ortho-monooxygenase of Burkholderia cepacia G4 for enhanced 1-naphthol synthesis and chloroform degradation (2004), Appl. Environ. Microbiol., 70, 3246-3252 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.13.243
-
 Burkholderia cepacia

Protein Variants

EC Number Protein Variants Comment Organism
1.14.13.243 A106E mutation in hydroxylase alpha-subunit TomA3, variant degrades its natural substrate toluene 63% faster than wild-type, with 50% 2-methylphenol, 25% 3-methylphenol, and 25% 4-methylphenol being formed. Whole cells expressing the A106E variant have two times better naphthalene-to-1-naphthol activity than the wild-type, and the regiospecificity of the A106E variant is unchanged, with 98% 1-naphthol formed Burkholderia cepacia

Organism

EC Number Organism UniProt Comment Textmining
1.14.13.243 Burkholderia cepacia Q9ANX4 and Q9ANX0 and Q9ANX3 and Q8VSV8 and Q9ANX1 and Q8VSV9 Q9ANX4 i.e. subunit tomA1, Q9ANX0 i.e. subunit tomA5, Q9ANX3 i.e. subunit TomA2, Q8VSV8 i.e. subunit TomA3, Q9ANX1 i.e. subunit TomA4, Q8VSV9 i.e. subunit TomA0
-
1.14.13.243 Burkholderia cepacia G4 Q9ANX4 and Q9ANX0 and Q9ANX3 and Q8VSV8 and Q9ANX1 and Q8VSV9 Q9ANX4 i.e. subunit tomA1, Q9ANX0 i.e. subunit tomA5, Q9ANX3 i.e. subunit TomA2, Q8VSV8 i.e. subunit TomA3, Q9ANX1 i.e. subunit TomA4, Q8VSV9 i.e. subunit TomA0
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.13.243 naphthalene + NADH + H+ + O2
-
 Burkholderia cepacia 1-naphthol + NAD+ + H2O
-
 ?
1.14.13.243 naphthalene + NADH + H+ + O2
-
 Burkholderia cepacia G4 1-naphthol + NAD+ + H2O
-
 ?
1.14.13.243 toluene + NADH + H+ + O2
-
 Burkholderia cepacia 2-methylphenol + NAD+ + H2O
-
 ?
1.14.13.243 toluene + NADH + H+ + O2
-
 Burkholderia cepacia G4 2-methylphenol + NAD+ + H2O
-
 ?