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Literature summary extracted from

  • Wagschal, K.C.; Rose Stoller, J.; Chan, V.J.; Jordan, D.B.
    Expression and characterization of hyperthermostable exopolygalacturonase RmGH28 from Rhodothermus marinus (2017), Appl. Biochem. Biotechnol., 183, 1503-1515 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.67 expressed in Escherichia coli BL21(DE3) cells Rhodothermus marinus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.67 digalacturonic acid substrate inhibition Rhodothermus marinus
3.2.1.67 galacturonic acid product inhibition Rhodothermus marinus
3.2.1.67 polygalacturonic acid substrate inhibition Rhodothermus marinus
3.2.1.67 trigalacturonic acid substrate inhibition Rhodothermus marinus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.67 0.000673
-
 polygalacturonic acid at pH 7.0 and 25°C Rhodothermus marinus
3.2.1.67 0.00241
-
 trigalacturonic acid at pH 7.0 and 25°C Rhodothermus marinus
3.2.1.67 0.012
-
 digalacturonic acid at pH 7.0 and 25°C Rhodothermus marinus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.67 citrus pectin + H2O Rhodothermus marinus
-
 galacturonic acid
-
 ?
3.2.1.67 citrus pectin + H2O Rhodothermus marinus ATCC 43812
-
 galacturonic acid
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.67 Rhodothermus marinus D0MEP1
-
-
3.2.1.67 Rhodothermus marinus ATCC 43812 D0MEP1
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.67 Ni-NTA column chromatography and Superdex 200 gel filtration Rhodothermus marinus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.67 citrus pectin + H2O
-
 Rhodothermus marinus galacturonic acid
-
 ?
3.2.1.67 citrus pectin + H2O
-
 Rhodothermus marinus ATCC 43812 galacturonic acid
-
 ?
3.2.1.67 digalacturonic acid + H2O
-
 Rhodothermus marinus 2 galacturonic acid
-
 ?
3.2.1.67 digalacturonic acid + H2O
-
 Rhodothermus marinus ATCC 43812 2 galacturonic acid
-
 ?
3.2.1.67 polygalacturonic acid + H2O
-
 Rhodothermus marinus galacturonic acid
-
 ?
3.2.1.67 polygalacturonic acid + H2O
-
 Rhodothermus marinus ATCC 43812 galacturonic acid
-
 ?
3.2.1.67 trigalacturonic acid + H2O
-
 Rhodothermus marinus digalacturonic acid + galacturonic acid
-
 ?
3.2.1.67 trigalacturonic acid + H2O
-
 Rhodothermus marinus ATCC 43812 digalacturonic acid + galacturonic acid
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.2.1.67 exopolygalacturonanase
-
 Rhodothermus marinus
3.2.1.67 GH28
-
 Rhodothermus marinus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.67 93.9
-
 50% of the initial enzyme activity remains after 1 h incubation at 93.9°C Rhodothermus marinus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2.1.67 6.02
-
 polygalacturonic acid at pH 7.0 and 25°C Rhodothermus marinus
3.2.1.67 11.7
-
 digalacturonic acid at pH 7.0 and 25°C Rhodothermus marinus
3.2.1.67 21.9
-
 trigalacturonic acid at pH 7.0 and 25°C Rhodothermus marinus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.67 7
-
-
 Rhodothermus marinus

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.2.1.67 0.000128
-
 galacturonic acid at pH 7.0 and 25°C Rhodothermus marinus
3.2.1.67 0.071
-
 polygalacturonic acid at pH 7.0 and 25°C Rhodothermus marinus
3.2.1.67 0.074
-
 trigalacturonic acid at pH 7.0 and 25°C Rhodothermus marinus
3.2.1.67 0.137
-
 digalacturonic acid at pH 7.0 and 25°C Rhodothermus marinus

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.2.1.67 968
-
 digalacturonic acid at pH 7.0 and 25°C Rhodothermus marinus
3.2.1.67 8950
-
 polygalacturonic acid at pH 7.0 and 25°C Rhodothermus marinus
3.2.1.67 9080
-
 trigalacturonic acid at pH 7.0 and 25°C Rhodothermus marinus