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Literature summary extracted from

  • Aggarwal, V.; Malik, J.; Prashant, A.; Jaiwal, P.K.; Pundir, C.S.
    Amperometric determination of serum total cholesterol with nanoparticles of cholesterol esterase and cholesterol oxidase (2016), Anal. Biochem., 500, 6-11 .
    View publication on PubMed

Application

EC Number Application Comment Organism
3.1.1.13 diagnostics glutaraldehyde cross-linked and functionalized cholesterol esterase and cholesterol oxidase nanoparticles (ChENPs) can be used as biosensors. The biosensor exhibits optimal response at pH 5.5 and 40°C within 5 s when polarized at +0.25 V versus Ag/AgCl. The working/linear range of the biosensor is 10-700 mg/dl for cholesterol. The sensor shows high sensitivity and measures total cholesterol as low as 0.1 mg/dl. The biosensor is evaluated and employed for total cholesterol determination in sera of apparently healthy and diseased persons. Measurement of total cholesterol level in serum is very important due to its presumed relationship to atherosclerosis and cardiovascular diseases, nephritis, myxedema, obstructive jaundice, diabetes mellitus, and cerebral thrombosis Sus scrofa

Protein Variants

EC Number Protein Variants Comment Organism
3.1.1.13 additional information immobilization of the enzyme by desolvation with ethanol and subsequent cross-linking with glutaraldehyde, preparation of glutaraldehyde cross-linked and functionalized cholesterol esterase nanoparticles (ChENPs), co-immobilization of cholesterol oxidase, method optimization and evaluation, overview Sus scrofa

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.13 Sus scrofa
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.1.13 commercial preparation
-
 Sus scrofa
-
3.1.1.13 pancreas
-
 Sus scrofa
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.13 cholesteryl acetate + H2O
-
 Sus scrofa cholesterol + acetate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.1.1.13 ChE
-
 Sus scrofa
3.1.1.13 cholesterol esterase
-
 Sus scrofa

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.1.13 37
-
 assay at Sus scrofa

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.1.13 7
-
 assay at Sus scrofa