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Literature summary extracted from

  • Dejtisakdi, W.; Miller, S.
    Overexpression of Calvin cycle enzyme fructose 1,6-bisphosphatase in Chlamydomonas reinhardtii has a detrimental effect on growth (2016), Algal Res., 14, 116-126 .
No PubMed abstract available

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.3.11 gene FBP1, recombinant expression of the FLAG-tagged plastidic enzyme from chloroplast expression vector pWD2-CpFBP1, that includes the Chlamydomonas reinhardtii FBP1 coding region synthesized with Chlamydomonas reinhardtii chloroplast codon-bias, flanked with psbD and psbA 5' and 3' regulatory sequences, respectively, in Chlamydomonas reinhardtii strain CC125 cells. Integration of insert from expression vector pWD2-CpFBP1 into the Chlamydomonas reinhardtii chloroplast genome. Under photoautotrophic growth conditions and/or with elevated CO2 levels, this 40% elevation in FBPase activity has a negative effect overall on growth rate and biomass production Chlamydomonas reinhardtii

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.3.11 chloroplast
-
 Chlamydomonas reinhardtii 9507
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.3.11 Mg2+ required Chlamydomonas reinhardtii

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.3.11 D-fructose 1,6-bisphosphate + H2O Chlamydomonas reinhardtii
-
 D-fructose 6-phosphate + phosphate
-
 ?
3.1.3.11 D-fructose 1,6-bisphosphate + H2O Chlamydomonas reinhardtii CC125
-
 D-fructose 6-phosphate + phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.3.11 Chlamydomonas reinhardtii A8IKQ0
-
-
3.1.3.11 Chlamydomonas reinhardtii CC125 A8IKQ0
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.3.11 D-fructose 1,6-bisphosphate + H2O
-
 Chlamydomonas reinhardtii D-fructose 6-phosphate + phosphate
-
 ?
3.1.3.11 D-fructose 1,6-bisphosphate + H2O
-
 Chlamydomonas reinhardtii CC125 D-fructose 6-phosphate + phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.3.11 ? x * 45000, recombinant FLAG-tagged enzyme CpFBP1, SDS-PAGE Chlamydomonas reinhardtii

Synonyms

EC Number Synonyms Comment Organism
3.1.3.11 CpFBP1
-
 Chlamydomonas reinhardtii
3.1.3.11 CrFBPase
-
 Chlamydomonas reinhardtii
3.1.3.11 cyanobacterial fructose-1,6-bisphosphatase
-
 Chlamydomonas reinhardtii
3.1.3.11 cyanoFBPase-II
-
 Chlamydomonas reinhardtii
3.1.3.11 FBP1 gene name, nuclear gene that encodes CrFBPase Chlamydomonas reinhardtii
3.1.3.11 fructose 1,6-bisphosphatase
-
 Chlamydomonas reinhardtii

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.3.11 25
-
 assay at Chlamydomonas reinhardtii

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.3.11 8.2
-
 assay at Chlamydomonas reinhardtii

General Information

EC Number General Information Comment Organism
3.1.3.11 metabolism Calvin cycle enzyme Chlamydomonas reinhardtii
3.1.3.11 metabolism the enzyme fructose 1,6-bisphosphatase takes part in the Calvin cycle, but unlike the situation in higher plants, FBPase probably does not catalyze a rate-limiting step in the Chlamydomonas reinhardtii Calvin cycle, since under photoautotrophic growth conditions and/or with elevated CO2 levels, an elevation in FBPase activity has a negative effect overall on growth rate and biomass production Chlamydomonas reinhardtii
3.1.3.11 physiological function overexpression of fructose 1,6-bisphosphatase has a detrimental effect on growth Chlamydomonas reinhardtii