EC Number | Cloned (Comment) | Organism |
---|---|---|
3.2.1.3 | recombinant expression of the starch-binding domain (SBD)-containing polypeptide (amino acids 509-616) of glucoamylase in Escherichia coli | Aspergillus niger |
EC Number | Crystallization (Comment) | Organism |
---|---|---|
3.2.1.3 | purified starch-binding domain (SBD)-containing polypeptide (amino acids 509-616) of Aspergillus niger glucoamylase, hanging drop vapour diffusion method, using 2.0-2.3 M ammonium sulfate, 1.7% w/v PEG 400, 15% v/v glycerol, and 85 mM HEPES, pH 7.5, as the reservoir solution, the asymmetric unit of the starch-binding domain is composed of four protein molecules, 20°C, two weeks, X-ray diffraction structure determination and analysis at 2.0 A resolution, NMR structures of the SBD of Aspergillus niger glucoamylase (PDB entries 1kum, 1kul, 1aco, and 1acz) are used as search models for molecular replacement | Aspergillus niger |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.2.1.3 | starch + H2O | Aspergillus niger | - |
? | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.2.1.3 | Aspergillus niger | P69328 | - |
- |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.2.1.3 | starch + H2O | - |
Aspergillus niger | ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
3.2.1.3 | More | the starch-binding domain (SBD) of glucoamylase from Aspergillus niger is a small globular protein containing a disulfide bond. The structure of the SBD has been determined by NMR. Cys509 and Cys604 are at the N- and C-termini, respectively, and they are linked by a disulfide bond, analysis of the conformation surrounding the disulfide bond, overview | Aspergillus niger |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.2.1.3 | GlaA | - |
Aspergillus niger |
3.2.1.3 | glucoamylase | - |
Aspergillus niger |