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Literature summary extracted from

  • Goswami, R.; Bondoc, J.M.G.; Wheeler, P.R.; Jafari, A.; Gonzalez, T.; Mehboob, S.; Movahedzadeh, F.
    Inositol monophosphatase A bifunctional enzyme in Mycobacterium smegmatis (2018), ACS Omega, 3, 13876-13881 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.3.11
-
 Mycolicibacterium smegmatis
3.1.3.25
-
 Mycolicibacterium smegmatis

Protein Variants

EC Number Protein Variants Comment Organism
3.1.3.11 D221A loss of 60-70% of activity for both FBPase and IMPase activities Mycolicibacterium smegmatis
3.1.3.11 G94A/T95A mutations partially restore the IMPase activity compared to the single mutants Mycolicibacterium smegmatis
3.1.3.11 T95A about 60% loss of both FBPase and IMPase activities Mycolicibacterium smegmatis
3.1.3.11 T95S about 35% loss of both FBPase and IMPase activities Mycolicibacterium smegmatis
3.1.3.11 W220A loss of 60-70% of activity for both FBPase and IMPase activities Mycolicibacterium smegmatis
3.1.3.11 W220A/D221A mutations reduces both FBPase and IMPase activity drastically Mycolicibacterium smegmatis
3.1.3.25 D221A loss of 60-70% of activity for both FBPase and IMPase activities Mycolicibacterium smegmatis
3.1.3.25 G94A/T95A mutations partially restore the IMPase activity compared to the single mutants Mycolicibacterium smegmatis
3.1.3.25 T95A about 60% loss of both FBPase and IMPase activities Mycolicibacterium smegmatis
3.1.3.25 T95S about 35% loss of both FBPase and IMPase activities Mycolicibacterium smegmatis
3.1.3.25 W220A loss of 60-70% of activity for both FBPase and IMPase activities Mycolicibacterium smegmatis
3.1.3.25 W220A/D221A mutations reduces both FBPase and IMPase activity drastically Mycolicibacterium smegmatis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.3.11 0.0111
-
 D-fructose 1,6-diphosphate pH 8.0, 23°C Mycolicibacterium smegmatis
3.1.3.25 0.0115
-
 Inositol 1-phosphate pH 8.0, 23°C Mycolicibacterium smegmatis

Organism

EC Number Organism UniProt Comment Textmining
3.1.3.11 Mycolicibacterium smegmatis A0QX86 bifunctional enzyme,also functions as a inositol monphosphatase, EC 3.1.3.25
-
3.1.3.11 Mycolicibacterium smegmatis ATCC 700084 A0QX86 bifunctional enzyme,also functions as a inositol monphosphatase, EC 3.1.3.25
-
3.1.3.25 Mycolicibacterium smegmatis A0QX86 bifunctional enzyme,also functions as a fructose-1,6-bisphosphatase, EC 3.1.3.11
-
3.1.3.25 Mycolicibacterium smegmatis ATCC 700084 A0QX86 bifunctional enzyme,also functions as a fructose-1,6-bisphosphatase, EC 3.1.3.11
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.3.11 D-fructose 1,6-diphosphate + H2O
-
 Mycolicibacterium smegmatis D-fructose 6-phosphate + phosphate
-
 ?
3.1.3.11 D-fructose 1,6-diphosphate + H2O
-
 Mycolicibacterium smegmatis ATCC 700084 D-fructose 6-phosphate + phosphate
-
 ?
3.1.3.25 inositol 1-phosphate + H2O
-
 Mycolicibacterium smegmatis inositol + phosphate
-
 ?
3.1.3.25 inositol 1-phosphate + H2O
-
 Mycolicibacterium smegmatis ATCC 700084 inositol + phosphate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.1.3.11 ImpA
-
 Mycolicibacterium smegmatis
3.1.3.11 IMPase A
-
 Mycolicibacterium smegmatis
3.1.3.25 ImpA
-
 Mycolicibacterium smegmatis
3.1.3.25 IMPase A
-
 Mycolicibacterium smegmatis