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Literature summary extracted from

  • Ducati, R.G.; Harijan, R.K.; Cameron, S.A.; Tyler, P.C.; Evans, G.B.; Schramm, V.L.
    Transition-state analogues of Campylobacter jejuni 5'-methylthioadenosine nucleosidase (2018), ACS Chem. Biol., 13, 3173-3183 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.2.26 expressed in Escherichia coli BL21(DE3) cells Campylobacter jejuni subsp. jejuni
3.2.2.30 expression in Escherichia coli Campylobacter jejuni subsp. jejuni serotype O:2

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.2.2.26 free enzyme or in complex with inhibitors, sitting drop vapor diffusion method Campylobacter jejuni subsp. jejuni
3.2.2.30 structure of apoenzyme at 1.25 A, and five enzyme complexes with transition-state analogues at 1.42 to 1.95 A resolution. Inhibitor binding induces a loop movement to create a closed catalytic site with Asp196 and Ile152 providing purine leaving group activation and Arg192 and Glu12 activating the water nucleophile Campylobacter jejuni subsp. jejuni serotype O:2

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.2.26 (2R)-2-[[(4-amino-5,7a-dihydro-4aH-pyrrolo[3,2-d]pyrimidin-7-yl)methyl]amino]-3-(methylsulfanyl)propan-1-ol
-
 Campylobacter jejuni subsp. jejuni
3.2.2.26 (2R)-2-[[(4-amino-5,7a-dihydro-4aH-pyrrolo[3,2-d]pyrimidin-7-yl)methyl]amino]-3-[(pyrazin-2-yl)sulfanyl]propan-1-ol
-
 Campylobacter jejuni subsp. jejuni
3.2.2.26 (2S)-2-[[(4-amino-5,7a-dihydro-4aH-pyrrolo[3,2-d]pyrimidin-7-yl)methyl]amino]octan-1-ol
-
 Campylobacter jejuni subsp. jejuni
3.2.2.26 (2S,3S,4R,5S)-2-(4-amino-5,7a-dihydro-4aH-pyrrolo[3,2-d]pyrimidin-7-yl)-5-[(methylsulfanyl)methyl]pyrrolidine-3,4-diol
-
 Campylobacter jejuni subsp. jejuni
3.2.2.26 (2S,3S,4R,5S)-2-(4-amino-5,7a-dihydro-4aH-pyrrolo[3,2-d]pyrimidin-7-yl)-5-[[(4-chlorophenyl)sulfanyl]methyl]pyrrolidine-3,4-diol
-
 Campylobacter jejuni subsp. jejuni
3.2.2.26 (2S,3S,4R,5S)-2-(4-amino-5,7a-dihydro-4aH-pyrrolo[3,2-d]pyrimidin-7-yl)-5-[[(naphthalen-1-yl)sulfanyl]methyl]pyrrolidine-3,4-diol
-
 Campylobacter jejuni subsp. jejuni
3.2.2.26 (3R,4R)-1-[(4-amino-5,7a-dihydro-4aH-pyrrolo[3,2-d]pyrimidin-7-yl)methyl]-4-(hydroxymethyl)pyrrolidin-3-ol
-
 Campylobacter jejuni subsp. jejuni
3.2.2.26 (3R,4S)-1-[(4-amino-5,7a-dihydro-4aH-pyrrolo[3,2-d]pyrimidin-7-yl)methyl]-4-(adamant-1-yl)sulfanyl]methyl]pyrrolidin-3-ol
-
 Campylobacter jejuni subsp. jejuni
3.2.2.26 (3R,4S)-1-[(4-amino-5,7a-dihydro-4aH-pyrrolo[3,2-d]pyrimidin-7-yl)methyl]-4-([[2-(2-hydroxyethoxy)ethyl]sulfanyl]methyl)pyrrolidin-3-ol
-
 Campylobacter jejuni subsp. jejuni
3.2.2.26 (3R,4S)-1-[(4-amino-5,7a-dihydro-4aH-pyrrolo[3,2-d]pyrimidin-7-yl)methyl]-4-butylpyrrolidin-3-ol
-
 Campylobacter jejuni subsp. jejuni
3.2.2.26 (3R,4S)-1-[(4-amino-5,7a-dihydro-4aH-pyrrolo[3,2-d]pyrimidin-7-yl)methyl]-4-ethylpyrrolidin-3-ol
-
 Campylobacter jejuni subsp. jejuni
3.2.2.26 (3R,4S)-1-[(4-amino-5,7a-dihydro-4aH-pyrrolo[3,2-d]pyrimidin-7-yl)methyl]-4-methylpyrrolidin-3-ol
-
 Campylobacter jejuni subsp. jejuni
3.2.2.26 (3R,4S)-1-[(4-amino-5,7a-dihydro-4aH-pyrrolo[3,2-d]pyrimidin-7-yl)methyl]-4-propylpyrrolidin-3-ol
-
 Campylobacter jejuni subsp. jejuni
3.2.2.26 (3R,4S)-1-[(4-amino-5,7a-dihydro-4aH-pyrrolo[3,2-d]pyrimidin-7-yl)methyl]-4-[(butylsulfanyl)methyl]pyrrolidin-3-ol
-
 Campylobacter jejuni subsp. jejuni
3.2.2.26 (3R,4S)-1-[(4-amino-5,7a-dihydro-4aH-pyrrolo[3,2-d]pyrimidin-7-yl)methyl]-4-[(cyclohexylsulfanyl)methyl]pyrrolidin-3-ol
-
 Campylobacter jejuni subsp. jejuni
3.2.2.26 (3R,4S)-1-[(4-amino-5,7a-dihydro-4aH-pyrrolo[3,2-d]pyrimidin-7-yl)methyl]-4-[(hexylsulfanyl)methyl]pyrrolidin-3-ol
-
 Campylobacter jejuni subsp. jejuni
3.2.2.26 (3R,4S)-1-[(4-amino-5,7a-dihydro-4aH-pyrrolo[3,2-d]pyrimidin-7-yl)methyl]-4-[(methylsulfanyl)methyl]pyrrolidin-3-ol
-
 Campylobacter jejuni subsp. jejuni
3.2.2.26 (3R,4S)-1-[(4-amino-5,7a-dihydro-4aH-pyrrolo[3,2-d]pyrimidin-7-yl)methyl]-4-[(phenylsulfanyl)methyl]pyrrolidin-3-ol
-
 Campylobacter jejuni subsp. jejuni
3.2.2.26 (3R,4S)-1-[(4-amino-5,7a-dihydro-4aH-pyrrolo[3,2-d]pyrimidin-7-yl)methyl]-4-[2-(hexylsulfanyl)ethyl]pyrrolidin-3-ol
-
 Campylobacter jejuni subsp. jejuni
3.2.2.26 (3R,4S)-1-[(4-amino-5,7a-dihydro-4aH-pyrrolo[3,2-d]pyrimidin-7-yl)methyl]-4-[2-[2-(2-hydroxyethoxy)ethoxy]ethyl]pyrrolidin-3-ol
-
 Campylobacter jejuni subsp. jejuni
3.2.2.26 (3R,4S)-1-[(4-amino-5,7a-dihydro-4aH-pyrrolo[3,2-d]pyrimidin-7-yl)methyl]-4-[[(4-chlorophenyl)sulfanyl]methyl]pyrrolidin-3-ol
-
 Campylobacter jejuni subsp. jejuni
3.2.2.26 (3R,4S)-1-[(4-amino-5,7a-dihydro-4aH-pyrrolo[3,2-d]pyrimidin-7-yl)methyl]-4-[[(pyrazin-2-yl)sulfanyl]methyl]pyrrolidin-3-ol
-
 Campylobacter jejuni subsp. jejuni
3.2.2.26 (3S,4R,5R)-2-(4-amino-5,7a-dihydro-4aH-pyrrolo[3,2-d]pyrimidin-7-yl)-5-(hydroxymethyl)pyrrolidine-3,4-diol
-
 Campylobacter jejuni subsp. jejuni
3.2.2.26 (3S,4R,5R)-2-[(4-amino-5,7a-dihydro-4aH-pyrrolo[3,2-d]pyrimidin-7-yl)methyl]-5-(hydroxymethyl)pyrrolidine-3,4-diol
-
 Campylobacter jejuni subsp. jejuni
3.2.2.26 3-(3-[(3S,4R)-1-[(4-amino-5,7a-dihydro-4aH-pyrrolo[3,2-d]pyrimidin-7-yl)methyl]-4-hydroxypyrrolidin-3-yl]propanoyl)benzoic acid
-
 Campylobacter jejuni subsp. jejuni
3.2.2.26 3-[3-[(2R,3R,4S,5S)-5-(4-amino-5,7a-dihydro-4aH-pyrrolo[3,2-d]pyrimidin-7-yl)-3,4-dihydroxypyrrolidin-2-yl]propanoyl]benzoic acid
-
 Campylobacter jejuni subsp. jejuni
3.2.2.26 7-(5-S-methyl-5-thio-beta-D-ribofuranosyl)-7H-pyrrolo[2,3-d]pyrimidin-4-amine
-
 Campylobacter jejuni subsp. jejuni
3.2.2.26 gentamicin
-
 Campylobacter jejuni subsp. jejuni
3.2.2.26 [(3S,4R)-1-[(4-amino-5,7a-dihydro-4aH-pyrrolo[3,2-d]pyrimidin-7-yl)methyl]-4-hydroxypyrrolidin-3-yl]methionine
-
 Campylobacter jejuni subsp. jejuni
3.2.2.30 (3R,4S)-1-[(4-amino-5H-pyrrolo[3,2-d]pyrimidin-7-yl)methyl]-4-[(butylsulfanyl)methyl]pyrrolidin-3-ol transition state analogue, compound inhibits bacterial growth with IC50 values in the low micromolar range Campylobacter jejuni subsp. jejuni serotype O:2
3.2.2.30 (3R,4S)-1-[(4-amino-5H-pyrrolo[3,2-d]pyrimidin-7-yl)methyl]-4-[(hexylsulfanyl)methyl]pyrrolidin-3-ol transition state analogue, compound inhibits bacterial growth with IC50 values in the low micromolar range Campylobacter jejuni subsp. jejuni serotype O:2
3.2.2.30 (3R,4S)-1-[(4-amino-5H-pyrrolo[3,2-d]pyrimidin-7-yl)methyl]-4-[[(2,3-dihydropyrazin-2-yl)sulfanyl]methyl]pyrrolidin-3-ol transition state analogue, compound inhibits bacterial growth with IC50 values in the low micromolar range Campylobacter jejuni subsp. jejuni serotype O:2

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.2.26 0.0035
-
 6-amino-6-deoxyfutalosine at pH 7.4 and 25°C Campylobacter jejuni subsp. jejuni
3.2.2.26 0.0071
-
 5'-methylthioadenosine at pH 7.4 and 25°C Campylobacter jejuni subsp. jejuni
3.2.2.26 0.0075
-
 adenosine at pH 7.4 and 25°C Campylobacter jejuni subsp. jejuni
3.2.2.26 0.0122
-
 S-5'-adenosyl-L-homocysteine at pH 7.4 and 25°C Campylobacter jejuni subsp. jejuni
3.2.2.26 0.0214
-
 5'-deoxyadenosine at pH 7.4 and 25°C Campylobacter jejuni subsp. jejuni
3.2.2.30 0.0035
-
 6-amino-6-deoxyfutalosine pH 7.4, 25°C Campylobacter jejuni subsp. jejuni serotype O:2
3.2.2.30 0.0071
-
 5'-methylthioadenosine pH 7.4, 25°C Campylobacter jejuni subsp. jejuni serotype O:2
3.2.2.30 0.0075
-
 adenosine pH 7.4, 25°C Campylobacter jejuni subsp. jejuni serotype O:2
3.2.2.30 0.0122
-
 S-5'-adenosyl-L-homocysteine pH 7.4, 25°C Campylobacter jejuni subsp. jejuni serotype O:2
3.2.2.30 0.0214
-
 5'-deoxyadenosine pH 7.4, 25°C Campylobacter jejuni subsp. jejuni serotype O:2

Organism

EC Number Organism UniProt Comment Textmining
3.2.2.26 Campylobacter jejuni subsp. jejuni Q0PC20
-
-
3.2.2.26 Campylobacter jejuni subsp. jejuni ATCC 700819 Q0PC20
-
-
3.2.2.30 Campylobacter jejuni subsp. jejuni serotype O:2 Q0PC20
-
-
3.2.2.30 Campylobacter jejuni subsp. jejuni serotype O:2 ATCC 700819 Q0PC20
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.2.26 Ni-NTA column chromatography Campylobacter jejuni subsp. jejuni

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.2.26 5'-deoxyadenosine + H2O
-
 Campylobacter jejuni subsp. jejuni ?
-
 ?
3.2.2.26 5'-deoxyadenosine + H2O
-
 Campylobacter jejuni subsp. jejuni ATCC 700819 ?
-
 ?
3.2.2.26 5'-methylthioadenosine + H2O
-
 Campylobacter jejuni subsp. jejuni ?
-
 ?
3.2.2.26 5'-methylthioadenosine + H2O
-
 Campylobacter jejuni subsp. jejuni ATCC 700819 ?
-
 ?
3.2.2.26 6-amino-6-deoxyfutalosine + H2O
-
 Campylobacter jejuni subsp. jejuni dehypoxanthine futalosine + adenine
-
 ?
3.2.2.26 6-amino-6-deoxyfutalosine + H2O
-
 Campylobacter jejuni subsp. jejuni ATCC 700819 dehypoxanthine futalosine + adenine
-
 ?
3.2.2.26 adenosine + H2O
-
 Campylobacter jejuni subsp. jejuni ?
-
 ?
3.2.2.26 adenosine + H2O
-
 Campylobacter jejuni subsp. jejuni ATCC 700819 ?
-
 ?
3.2.2.26 S-5'-adenosyl-L-homocysteine + H2O
-
 Campylobacter jejuni subsp. jejuni ?
-
 ?
3.2.2.26 S-5'-adenosyl-L-homocysteine + H2O
-
 Campylobacter jejuni subsp. jejuni ATCC 700819 ?
-
 ?
3.2.2.30 5'-deoxyadenosine + H2O
-
 Campylobacter jejuni subsp. jejuni serotype O:2 adenine + 5-deoxyribose
-
 ?
3.2.2.30 5'-deoxyadenosine + H2O
-
 Campylobacter jejuni subsp. jejuni serotype O:2 ATCC 700819 adenine + 5-deoxyribose
-
 ?
3.2.2.30 5'-methylthioadenosine + H2O
-
 Campylobacter jejuni subsp. jejuni serotype O:2 adenine + S-methyl-5'-thioribose
-
 ?
3.2.2.30 5'-methylthioadenosine + H2O
-
 Campylobacter jejuni subsp. jejuni serotype O:2 ATCC 700819 adenine + S-methyl-5'-thioribose
-
 ?
3.2.2.30 6-amino-6-deoxyfutalosine + H2O
-
 Campylobacter jejuni subsp. jejuni serotype O:2 adenine + dehypoxanthinyl futalosine
-
 ?
3.2.2.30 6-amino-6-deoxyfutalosine + H2O
-
 Campylobacter jejuni subsp. jejuni serotype O:2 ATCC 700819 adenine + dehypoxanthinyl futalosine
-
 ?
3.2.2.30 adenosine + H2O
-
 Campylobacter jejuni subsp. jejuni serotype O:2 adenine + D-ribose
-
 ?
3.2.2.30 adenosine + H2O
-
 Campylobacter jejuni subsp. jejuni serotype O:2 ATCC 700819 adenine + D-ribose
-
 ?
3.2.2.30 S-5'-adenosyl-L-homocysteine + H2O
-
 Campylobacter jejuni subsp. jejuni serotype O:2 adenine + S-ribosyl-L-homocysteine
-
 ?
3.2.2.30 S-5'-adenosyl-L-homocysteine + H2O
-
 Campylobacter jejuni subsp. jejuni serotype O:2 ATCC 700819 adenine + S-ribosyl-L-homocysteine
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.2.2.26 5'-methylthioadenosine nucleosidase
-
 Campylobacter jejuni subsp. jejuni
3.2.2.26 MTAN
-
 Campylobacter jejuni subsp. jejuni

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2.2.26 0.27
-
 6-amino-6-deoxyfutalosine at pH 7.4 and 25°C Campylobacter jejuni subsp. jejuni
3.2.2.26 0.3
-
 adenosine at pH 7.4 and 25°C Campylobacter jejuni subsp. jejuni
3.2.2.26 0.55
-
 S-5'-adenosyl-L-homocysteine at pH 7.4 and 25°C Campylobacter jejuni subsp. jejuni
3.2.2.26 0.8
-
 5'-methylthioadenosine at pH 7.4 and 25°C Campylobacter jejuni subsp. jejuni
3.2.2.26 0.92
-
 5'-deoxyadenosine at pH 7.4 and 25°C Campylobacter jejuni subsp. jejuni
3.2.2.30 0.27
-
 6-amino-6-deoxyfutalosine pH 7.4, 25°C Campylobacter jejuni subsp. jejuni serotype O:2
3.2.2.30 0.3
-
 adenosine pH 7.4, 25°C Campylobacter jejuni subsp. jejuni serotype O:2
3.2.2.30 0.55
-
 S-5'-adenosyl-L-homocysteine pH 7.4, 25°C Campylobacter jejuni subsp. jejuni serotype O:2
3.2.2.30 0.8
-
 5'-methylthioadenosine pH 7.4, 25°C Campylobacter jejuni subsp. jejuni serotype O:2
3.2.2.30 0.92
-
 5'-deoxyadenosine pH 7.4, 25°C Campylobacter jejuni subsp. jejuni serotype O:2

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.2.2.26 0.00000065
-
 (3R,4S)-1-[(4-amino-5,7a-dihydro-4aH-pyrrolo[3,2-d]pyrimidin-7-yl)methyl]-4-[(hexylsulfanyl)methyl]pyrrolidin-3-ol at pH 7.4 and 25°C Campylobacter jejuni subsp. jejuni
3.2.2.26 0.00000075
-
 (3R,4S)-1-[(4-amino-5,7a-dihydro-4aH-pyrrolo[3,2-d]pyrimidin-7-yl)methyl]-4-[(butylsulfanyl)methyl]pyrrolidin-3-ol at pH 7.4 and 25°C Campylobacter jejuni subsp. jejuni
3.2.2.26 0.0000014
-
 (3R,4S)-1-[(4-amino-5,7a-dihydro-4aH-pyrrolo[3,2-d]pyrimidin-7-yl)methyl]-4-[[(pyrazin-2-yl)sulfanyl]methyl]pyrrolidin-3-ol at pH 7.4 and 25°C Campylobacter jejuni subsp. jejuni
3.2.2.26 0.0000014
-
 (3R,4S)-1-[(4-amino-5,7a-dihydro-4aH-pyrrolo[3,2-d]pyrimidin-7-yl)methyl]-4-butylpyrrolidin-3-ol at pH 7.4 and 25°C Campylobacter jejuni subsp. jejuni
3.2.2.26 0.000002
-
 (3R,4S)-1-[(4-amino-5,7a-dihydro-4aH-pyrrolo[3,2-d]pyrimidin-7-yl)methyl]-4-[2-(hexylsulfanyl)ethyl]pyrrolidin-3-ol at pH 7.4 and 25°C Campylobacter jejuni subsp. jejuni
3.2.2.26 0.0000022
-
 (3R,4S)-1-[(4-amino-5,7a-dihydro-4aH-pyrrolo[3,2-d]pyrimidin-7-yl)methyl]-4-[(cyclohexylsulfanyl)methyl]pyrrolidin-3-ol at pH 7.4 and 25°C Campylobacter jejuni subsp. jejuni
3.2.2.26 0.0000029
-
 (3R,4S)-1-[(4-amino-5,7a-dihydro-4aH-pyrrolo[3,2-d]pyrimidin-7-yl)methyl]-4-propylpyrrolidin-3-ol at pH 7.4 and 25°C Campylobacter jejuni subsp. jejuni
3.2.2.26 0.0000029
-
 (3R,4S)-1-[(4-amino-5,7a-dihydro-4aH-pyrrolo[3,2-d]pyrimidin-7-yl)methyl]-4-[(methylsulfanyl)methyl]pyrrolidin-3-ol at pH 7.4 and 25°C Campylobacter jejuni subsp. jejuni
3.2.2.26 0.0000031
-
 (3R,4S)-1-[(4-amino-5,7a-dihydro-4aH-pyrrolo[3,2-d]pyrimidin-7-yl)methyl]-4-[[(4-chlorophenyl)sulfanyl]methyl]pyrrolidin-3-ol at pH 7.4 and 25°C Campylobacter jejuni subsp. jejuni
3.2.2.26 0.0000032
-
 (3R,4S)-1-[(4-amino-5,7a-dihydro-4aH-pyrrolo[3,2-d]pyrimidin-7-yl)methyl]-4-([[2-(2-hydroxyethoxy)ethyl]sulfanyl]methyl)pyrrolidin-3-ol at pH 7.4 and 25°C Campylobacter jejuni subsp. jejuni
3.2.2.26 0.000004
-
 (3R,4S)-1-[(4-amino-5,7a-dihydro-4aH-pyrrolo[3,2-d]pyrimidin-7-yl)methyl]-4-[(phenylsulfanyl)methyl]pyrrolidin-3-ol at pH 7.4 and 25°C Campylobacter jejuni subsp. jejuni
3.2.2.26 0.0000062
-
 (2S)-2-[[(4-amino-5,7a-dihydro-4aH-pyrrolo[3,2-d]pyrimidin-7-yl)methyl]amino]octan-1-ol at pH 7.4 and 25°C Campylobacter jejuni subsp. jejuni
3.2.2.26 0.00001
-
 (3R,4S)-1-[(4-amino-5,7a-dihydro-4aH-pyrrolo[3,2-d]pyrimidin-7-yl)methyl]-4-ethylpyrrolidin-3-ol at pH 7.4 and 25°C Campylobacter jejuni subsp. jejuni
3.2.2.26 0.000014
-
 (2S,3S,4R,5S)-2-(4-amino-5,7a-dihydro-4aH-pyrrolo[3,2-d]pyrimidin-7-yl)-5-[(methylsulfanyl)methyl]pyrrolidine-3,4-diol at pH 7.4 and 25°C Campylobacter jejuni subsp. jejuni
3.2.2.26 0.000019
-
 (3R,4S)-1-[(4-amino-5,7a-dihydro-4aH-pyrrolo[3,2-d]pyrimidin-7-yl)methyl]-4-(adamant-1-yl)sulfanyl]methyl]pyrrolidin-3-ol at pH 7.4 and 25°C Campylobacter jejuni subsp. jejuni
3.2.2.26 0.000024
-
 [(3S,4R)-1-[(4-amino-5,7a-dihydro-4aH-pyrrolo[3,2-d]pyrimidin-7-yl)methyl]-4-hydroxypyrrolidin-3-yl]methionine at pH 7.4 and 25°C Campylobacter jejuni subsp. jejuni
3.2.2.26 0.000028
-
 (2S,3S,4R,5S)-2-(4-amino-5,7a-dihydro-4aH-pyrrolo[3,2-d]pyrimidin-7-yl)-5-[[(4-chlorophenyl)sulfanyl]methyl]pyrrolidine-3,4-diol at pH 7.4 and 25°C Campylobacter jejuni subsp. jejuni
3.2.2.26 0.000055
-
 (3R,4S)-1-[(4-amino-5,7a-dihydro-4aH-pyrrolo[3,2-d]pyrimidin-7-yl)methyl]-4-methylpyrrolidin-3-ol at pH 7.4 and 25°C Campylobacter jejuni subsp. jejuni
3.2.2.26 0.000106
-
 3-(3-[(3S,4R)-1-[(4-amino-5,7a-dihydro-4aH-pyrrolo[3,2-d]pyrimidin-7-yl)methyl]-4-hydroxypyrrolidin-3-yl]propanoyl)benzoic acid at pH 7.4 and 25°C Campylobacter jejuni subsp. jejuni
3.2.2.26 0.000216
-
 (2R)-2-[[(4-amino-5,7a-dihydro-4aH-pyrrolo[3,2-d]pyrimidin-7-yl)methyl]amino]-3-(methylsulfanyl)propan-1-ol at pH 7.4 and 25°C Campylobacter jejuni subsp. jejuni
3.2.2.26 0.000422
-
 (2S,3S,4R,5S)-2-(4-amino-5,7a-dihydro-4aH-pyrrolo[3,2-d]pyrimidin-7-yl)-5-[[(naphthalen-1-yl)sulfanyl]methyl]pyrrolidine-3,4-diol at pH 7.4 and 25°C Campylobacter jejuni subsp. jejuni
3.2.2.26 0.000946
-
 (2R)-2-[[(4-amino-5,7a-dihydro-4aH-pyrrolo[3,2-d]pyrimidin-7-yl)methyl]amino]-3-[(pyrazin-2-yl)sulfanyl]propan-1-ol at pH 7.4 and 25°C Campylobacter jejuni subsp. jejuni
3.2.2.26 0.0017
-
 (3R,4S)-1-[(4-amino-5,7a-dihydro-4aH-pyrrolo[3,2-d]pyrimidin-7-yl)methyl]-4-[2-[2-(2-hydroxyethoxy)ethoxy]ethyl]pyrrolidin-3-ol at pH 7.4 and 25°C Campylobacter jejuni subsp. jejuni
3.2.2.26 0.0026
-
 (3R,4R)-1-[(4-amino-5,7a-dihydro-4aH-pyrrolo[3,2-d]pyrimidin-7-yl)methyl]-4-(hydroxymethyl)pyrrolidin-3-ol at pH 7.4 and 25°C Campylobacter jejuni subsp. jejuni
3.2.2.26 0.0027
-
 3-[3-[(2R,3R,4S,5S)-5-(4-amino-5,7a-dihydro-4aH-pyrrolo[3,2-d]pyrimidin-7-yl)-3,4-dihydroxypyrrolidin-2-yl]propanoyl]benzoic acid at pH 7.4 and 25°C Campylobacter jejuni subsp. jejuni
3.2.2.26 0.013
-
 (3S,4R,5R)-2-[(4-amino-5,7a-dihydro-4aH-pyrrolo[3,2-d]pyrimidin-7-yl)methyl]-5-(hydroxymethyl)pyrrolidine-3,4-diol at pH 7.4 and 25°C Campylobacter jejuni subsp. jejuni
3.2.2.26 0.014
-
 (3S,4R,5R)-2-(4-amino-5,7a-dihydro-4aH-pyrrolo[3,2-d]pyrimidin-7-yl)-5-(hydroxymethyl)pyrrolidine-3,4-diol at pH 7.4 and 25°C Campylobacter jejuni subsp. jejuni
3.2.2.26 0.022
-
 7-(5-S-methyl-5-thio-beta-D-ribofuranosyl)-7H-pyrrolo[2,3-d]pyrimidin-4-amine at pH 7.4 and 25°C Campylobacter jejuni subsp. jejuni
3.2.2.30 0.0000033
-
 (3R,4S)-1-[(4-amino-5H-pyrrolo[3,2-d]pyrimidin-7-yl)methyl]-4-[(hexylsulfanyl)methyl]pyrrolidin-3-ol pH 7.4, 25°C Campylobacter jejuni subsp. jejuni serotype O:2
3.2.2.30 0.0000041
-
 (3R,4S)-1-[(4-amino-5H-pyrrolo[3,2-d]pyrimidin-7-yl)methyl]-4-[(butylsulfanyl)methyl]pyrrolidin-3-ol pH 7.4, 25°C Campylobacter jejuni subsp. jejuni serotype O:2
3.2.2.30 0.0000057
-
 (3R,4S)-1-[(4-amino-5H-pyrrolo[3,2-d]pyrimidin-7-yl)methyl]-4-[[(2,3-dihydropyrazin-2-yl)sulfanyl]methyl]pyrrolidin-3-ol pH 7.4, 25°C Campylobacter jejuni subsp. jejuni serotype O:2

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
3.2.2.26 0.0021
-
 at pH 7.4 and 25°C Campylobacter jejuni subsp. jejuni gentamicin

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.2.2.26 40
-
 adenosine at pH 7.4 and 25°C Campylobacter jejuni subsp. jejuni
3.2.2.26 43
-
 5'-deoxyadenosine at pH 7.4 and 25°C Campylobacter jejuni subsp. jejuni
3.2.2.26 46
-
 S-5'-adenosyl-L-homocysteine at pH 7.4 and 25°C Campylobacter jejuni subsp. jejuni
3.2.2.26 78
-
 6-amino-6-deoxyfutalosine at pH 7.4 and 25°C Campylobacter jejuni subsp. jejuni
3.2.2.26 113
-
 5'-methylthioadenosine at pH 7.4 and 25°C Campylobacter jejuni subsp. jejuni
3.2.2.30 40
-
 adenosine pH 7.4, 25°C Campylobacter jejuni subsp. jejuni serotype O:2
3.2.2.30 43
-
 5'-deoxyadenosine pH 7.4, 25°C Campylobacter jejuni subsp. jejuni serotype O:2
3.2.2.30 46
-
 S-5'-adenosyl-L-homocysteine pH 7.4, 25°C Campylobacter jejuni subsp. jejuni serotype O:2
3.2.2.30 78
-
 6-amino-6-deoxyfutalosine pH 7.4, 25°C Campylobacter jejuni subsp. jejuni serotype O:2
3.2.2.30 113
-
 5'-methylthioadenosine pH 7.4, 25°C Campylobacter jejuni subsp. jejuni serotype O:2