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Literature summary extracted from

  • Anand, G.; Yadav, S.; Yadav, D.
    Production, purification and biochemical characterization of an exo-polygalacturonase from Aspergillus niger MTCC 478 suitable for clarification of orange juice (2017), 3 Biotech, 7, 122 .
    View publication on PubMedView publication on EuropePMC

Application

EC Number Application Comment Organism
3.2.1.67 food industry the enzyme is suitable for clarification of orange juice Aspergillus niger

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.67 Ag+ 14.4% residual activity at 1 mM Aspergillus niger
3.2.1.67 Ca2+ 34% residual activity at 1 mM Aspergillus niger
3.2.1.67 Co2+ 80% residual activity at 1 mM Aspergillus niger
3.2.1.67 EDTA 61% residual activity at 1 mM Aspergillus niger
3.2.1.67 K+ 36% residual activity at 1 mM Aspergillus niger
3.2.1.67 additional information not inhibited by potassium permanganate Aspergillus niger
3.2.1.67 potassium ferrocyanide 65% residual activity at 1 mM Aspergillus niger
3.2.1.67 Zn2+ 38% residual activity at 1 mM Aspergillus niger

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.67 Cu2+ 148% activity at 1 mM Aspergillus niger
3.2.1.67 additional information not influenced by Hg2+, Na+ and Mn2+ Aspergillus niger

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.67 Aspergillus niger
-
-
-
3.2.1.67 Aspergillus niger MTCC 478
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.67 acetone precipitation and carboxymethyl cellulose column chromatography Aspergillus niger

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.67 polygalacturonic acid + H2O
-
 Aspergillus niger galacturonic acid
-
 ?
3.2.1.67 polygalacturonic acid + H2O
-
 Aspergillus niger MTCC 478 galacturonic acid
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.67 ? x * 124000, SDS-PAGE Aspergillus niger

Synonyms

EC Number Synonyms Comment Organism
3.2.1.67 Exo-PG
-
 Aspergillus niger
3.2.1.67 exo-polygalacturonase
-
 Aspergillus niger

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.67 50
-
-
 Aspergillus niger

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.2.1.67 30 70 more than 60% activity between 30 and 70°C Aspergillus niger

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.67 10 50 the enzyme retains its maximum activity between 10 and 40°C for 30 min. The maximum stability is up to 40°C and the activity declines sharply above this temperature. Approximately 53% of the activity is retained at 50°C Aspergillus niger

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2.1.67 194
-
 polygalacturonic acid at pH 4.0 and 40°C Aspergillus niger

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.67 4
-
-
 Aspergillus niger

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.67 3 11 the enzyme is stable for pH values of 3.0-11.0 when exposed to buffers of various pH for 24 h Aspergillus niger